Method for culturing mammalian cells in a perfused bioreactor

A bio-reactor system wherein a tubular housing contains an internal circularly disposed set of blade members and a central tubular filter all mounted for rotation about a common horizontal axis and each having independent rotational support and rotational drive mechanisms. The housing, blade members and filter preferably are driven at a constant slow speed for placing a fluid culture medium with discrete microbeads and cell cultures in a discrete spatial suspension in the housing. Replacement fluid medium is symmetrically input and fluid medium is symmetrically output from the housing where the input and the output are part of a loop providing a constant or intermittent flow of fluid medium in a closed loop

Rotating bio-reactor cell culture apparatus

A bioreactor system is described in which a tubular housing contains an internal circularly disposed set of blade members and a central tubular filter all mounted for rotation about a common horizontal axis and each having independent rotational support and rotational drive mechanisms. The housing, blade members and filter preferably are driven at a constant slow speed for placing a fluid culture medium with discrete microbeads and cell cultures in a discrete spatial suspension in the housing. Replacement fluid medium is symmetrically input and fluid medium is symmetrically output from the housing where the input and the output are part of a loop providing a constant or intermittent flow of fluid medium in a closed loop

Automatic instrument for chemical processing to detect microorganism in biological samples by measuring light reactions

An automated apparatus is reported for sequentially assaying urine samples for the presence of bacterial adenosine triphosphate (ATP) that comprises a rotary table which carries a plurality of sample containing vials and automatically dispenses fluid reagents into the vials preparatory to injecting a light producing luciferase-luciferin mixture into the samples. The device automatically measures the light produced in each urine sample by a bioluminescence reaction of the free bacterial adenosine triphosphate with the luciferase-luciferin mixture. The light measured is proportional to the concentration of bacterial adenosine triphosphate which, in turn, is proportional to the number of bacteria present in the respective urine sample

Actuation, Sensing And Control For Micro Bio Robots

The continuing trend in miniaturization of technology, advancements in micro and nanofabrication and improvements in high-resolution imaging has enabled micro- and meso-scale robots that have many applications. They can be used for micro-assembly, directed drug delivery, microsurgery and high-resolution measurement. In order to create microrobots, microscopic sensors, actuators and controllers are needed. Unique challenges arise when building microscale robots. For inspiration, we look toward highly capable biological organisms, which excel at these length scales. In this dissertation we develop technologies that combine biological components and synthetic components to create actuation, sensing and assembly onboard microrobots. For actuation, we study the dynamics of synthetic micro structures that have been integrated with single-cell biological organisms to provide un-tethered onboard propulsion to the microrobot. For sensing, we integrate synthetically engineered sensor cells to enable a system capable of detecting a change in the local environment, then storing and reporting the information. Furthermore, we develop a bottom-up fabrication method using a macroscopic magnetic robot to direct the assembly of inorganic engineered micro structures. We showcase the capability of this assembly method by demonstrating highly-specified, predictable assembly of microscale building blocks in a semi-autonomous experiment. These magnetic robots can be used to program the assembly of passive building blocks, with the building blocks themselves having the potential to be arbitrarily complex. We extend the magnetic robot actuation work to consider control algorithms for multiple robots by exploiting spatial gradients of magnetic fields. This thesis makes contributions toward actuation, sensing and control of autonomous micro systems and provides technologies that will lead to the development of swarms of microrobots with a suite of manipulation and sensing capabilities working together to sense and modify the environment

Automated biowaste sampling system urine subsystem operating model, part 1

The urine subsystem automatically provides for the collection, volume sensing, and sampling of urine from six subjects during space flight. Verification of the subsystem design was a primary objective of the current effort which was accomplished thru the detail design, fabrication, and verification testing of an operating model of the subsystem

NASA patent abstracts bibliography: A continuing bibliography. Section 1: Abstracts (supplement 14)

Abstracts are cited for 213 patents and applications for patent introduced into the NASA scientific and technical information system during the period of July 1978 through December 1978. Each entry consists of a citation, an abstract, and in most cases, a key illustration selected from the patent or application for patent

The Effect of Flagella Stiffness on the Locomotion of a Multi-Flagellated Robot at Low Reynolds Environment

Microorganisms such as algae and bacteria move in a viscous environment with extremely low Reynolds ($Re$), where the viscous drag dominates the inertial forces. They have adapted to this environment by developing specialized features such as whole-body deformations and flexible structures such as flagella (with various shapes, sizes, and numbers) that break the symmetry during the motion. In this study, we hypothesize that the changes in the flexibility of the flagella during a cycle of movement impact locomotion dynamics of flagellated locomotion. To test our hypothesis, we developed an autonomous, self-propelled robot with four flexible, multi-segmented flagella actuated together by a single DC motor. The stiffness of the flagella during the locomotion is controlled via a cable-driven mechanism attached to the center of the robot. Experimental assessments of the robot's swimming demonstrate that increasing the flexibility of the flagella during recovery stroke and reducing the flexibility during power stroke improves the swimming performance of the robot. Our results give insight into how these microorganisms manipulate their biological features to propel themselves in low viscous media and are of great interest to biomedical and research applications

Cleaning devices

Cleaning devices are described which include a vacuum cleaner wherein electrostatically charged brushes that brush dirt off a floor, are electrically grounded to remove charges that could tend to hold dirt to the brushes

Hand Strength and Dexterity Enhancer

Good Grips created the hand strength and dexterity enhancer project to meet the challenge presented by Bill Phelps. He has Inclusion Body Myositis (IBM), an inflammatory muscle disease characterized by progressive muscle weakness and atrophy. Bill has limited strength in both hands and lost the ability to bend his fingers, making it difficult to grip anything. He needed a device that would help with everyday tasks such as writing, lifting, and holding various objects. Our team consists of four engineering students attending Cal Poly, San Luis Obispo. We researched, designed, manufactured, and tested a device that would fulfill the problem requirements for three quarters. Our device consists of two main components: a pair of custom gloves and a fanny pack that houses our electrical control system