Veni Vidi Vici, A Three-Phase Scenario For Parameter Space Analysis in Image Analysis and Visualization

Automatic analysis of the enormous sets of images is a critical task in life sciences. This faces many challenges such as: algorithms are highly parameterized, significant human input is intertwined, and lacking a standard meta-visualization approach. This paper proposes an alternative iterative approach for optimizing input parameters, saving time by minimizing the user involvement, and allowing for understanding the workflow of algorithms and discovering new ones. The main focus is on developing an interactive visualization technique that enables users to analyze the relationships between sampled input parameters and corresponding output. This technique is implemented as a prototype called Veni Vidi Vici, or "I came, I saw, I conquered." This strategy is inspired by the mathematical formulas of numbering computable functions and is developed atop ImageJ, a scientific image processing program. A case study is presented to investigate the proposed framework. Finally, the paper explores some potential future issues in the application of the proposed approach in parameter space analysis in visualization

Shedding light on melanins within in situ human eye melanocytes using 2-photon microscopy profiling techniques.

Choroidal melanocytes (HCMs) are melanin-producing cells in the vascular uvea of the human eye (iris, ciliary body and choroid). These cranial neural crest-derived cells migrate to populate a mesodermal microenvironment, and display cellular functions and extracellular interactions that are biologically distinct to skin melanocytes. HCMs (and melanins) are important in normal human eye physiology with roles including photoprotection, regulation of oxidative damage and immune responses. To extend knowledge of cytoplasmic melanins and melanosomes in label-free HCMs, a non-invasive 'fit-free' approach, combining 2-photon excitation fluorescence lifetimes and emission spectral imaging with phasor plot segmentation was applied. Intracellular melanin-mapped FLIM phasors showed a linear distribution indicating that HCM melanins are a ratio of two fluorophores, eumelanin and pheomelanin. A quantitative histogram of HCM melanins was generated by identifying the image pixel fraction contributed by phasor clusters mapped to varying eumelanin/pheomelanin ratio. Eumelanin-enriched dark HCM regions mapped to phasors with shorter lifetimes and longer spectral emission (580-625 nm) and pheomelanin-enriched lighter pigmented HCM regions mapped to phasors with longer lifetimes and shorter spectral emission (550-585 nm). Overall, we demonstrated that these methods can identify and quantitatively profile the heterogeneous eumelanins/pheomelanins within in situ HCMs, and visualize melanosome spatial distributions, not previously reported for these cells

Effect of Uveal Melanocytes on Choroidal Morphology in Rhesus Macaques and Humans on Enhanced-Depth Imaging Optical Coherence Tomography.

PurposeTo compare cross-sectional choroidal morphology in rhesus macaque and human eyes using enhanced-depth imaging optical coherence tomography (EDI-OCT) and histologic analysis.MethodsEnhanced-depth imaging-OCT images from 25 rhesus macaque and 30 human eyes were evaluated for choriocapillaris and choroidal-scleral junction (CSJ) visibility in the central macula based on OCT reflectivity profiles, and compared with age-matched histologic sections. Semiautomated segmentation of the choriocapillaris and CSJ was used to measure choriocapillary and choroidal thickness, respectively. Multivariate regression was performed to determine the association of age, refractive error, and race with choriocapillaris and CSJ visibility.ResultsRhesus macaques exhibit a distinct hyporeflective choriocapillaris layer on EDI-OCT, while the CSJ cannot be visualized. In contrast, humans show variable reflectivities of the choriocapillaris, with a distinct CSJ seen in many subjects. Histologic sections demonstrate large, darkly pigmented melanocytes that are densely distributed in the macaque choroid, while melanocytes in humans are smaller, less pigmented, and variably distributed. Optical coherence tomography reflectivity patterns of the choroid appear to correspond to the density, size, and pigmentation of choroidal melanocytes. Mean choriocapillary thickness was similar between the two species (19.3 ± 3.4 vs. 19.8 ± 3.4 μm, P = 0.615), but choroidal thickness may be lower in macaques than in humans (191.2 ± 43.0 vs. 266.8 ± 78.0 μm, P < 0.001). Racial differences in uveal pigmentation also appear to affect the visibility of the choriocapillaris and CSJ on EDI-OCT.ConclusionsPigmented uveal melanocytes affect choroidal morphology on EDI-OCT in rhesus macaque and human eyes. Racial differences in pigmentation may affect choriocapillaris and CSJ visibility, and may influence the accuracy of choroidal thickness measurements

Binary object recognition system on FPGA with bSOM

Tri-state Self Organizing Map (bSOM), which takes binary inputs and maintains tri-state weights, has been used for classification rather than clustering in this paper. The major contribution here is the demonstration of the potential use of the modified bSOM in security surveillance, as a recognition system on FPGA

Towards Accurate Pupil Detection Based on Morphology and Hough Transform

التعرف التلقائي على الأفراد مهم للغاية في العصور الحديثة. ظهرت تقنيات القياس الحيوي كإجابة على مسألة التعرف الفردي التلقائي. تميل هذه الورقة إلى إعطاء تقنية لاكتشاف البؤبؤ وهي مزيج من العمليات المورفولوجية السهلة ، و تحويل Hough (HT) . يتم تقسيم المنطقة الدائرية للعين والبؤبؤ بواسطة المرشح المورفولوجي وكذلك تحويل Hough حيث تم تحويل منطقة Iris القزحية المحلية إلى كتلة مستطيلة لغرض حساب التناقضات في الصورة. يتم تنفيذ هذه الطريقة واختبارها على قاعدة بيانات صور قزحية الأكاديمية الصينية للعلوم(CASIA V4)  لـ 249  شخص وقاعدة بيانات IIT Delhi (IITD) iris v1 باستخدام ماتلاب  MATLAB 2017a  . تتميز هذه الطريقة بدقة عالية في ايجاد المركز وتبلغ نسبة الوصول إلى دائرة نصف قطرها 97٪ لـ 2268 قزحية على صور CASIA V4 و 99.77٪ لصور قزحية 2240 على IITD، والسرعة مقبولة مقارنة بسرعة الكشف في الوقت الحقيقي والأداء المستقر. Automatic recognition of individuals is very important in modern eras. Biometric techniques have emerged as an answer to the matter of automatic individual recognition. This paper tends to give a technique to detect pupil which is a mixture of easy morphological operations and Hough Transform (HT) is presented in this paper. The circular area of the eye and pupil is divided by the morphological filter as well as the Hough Transform (HT) where the local Iris area has been converted into a rectangular block for the purpose of calculating inconsistencies in the image. This method is implemented and tested on the Chinese Academy of Sciences (CASIA V4) iris image database 249 person and the IIT Delhi (IITD) iris database v1 using MATLAB 2017a. This method has high accuracy in the center and radius finding reaches 97% for 2268 iris on CASIA V4 image and 99.77% for 2240 iris images on IITD, the speed is acceptable compared to the real-time detection speed and stable performance

A hybrid method for accurate iris segmentation on at-a-distance visible-wavelength images

[EN] This work describes a new hybrid method for accurate iris segmentation from full-face images independently of the ethnicity of the subject. It is based on a combination of three methods: facial key-point detection, integro-differential operator (IDO) and mathematical morphology. First, facial landmarks are extracted by means of the Chehra algorithm in order to obtain the eye location. Then, the IDO is applied to the extracted sub-image containing only the eye in order to locate the iris. Once the iris is located, a series of mathematical morphological operations is performed in order to accurately segment it. Results are obtained and compared among four different ethnicities (Asian, Black, Latino and White) as well as with two other iris segmentation algorithms. In addition, robustness against rotation, blurring and noise is also assessed. Our method obtains state-of-the-art performance and shows itself robust with small amounts of blur, noise and/or rotation. 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