Design and Implementation of a Three Dimensional Printer Using a Cylindrical Printing Process

Many 3D printers utilize a Cartesian (XYZ) printing process. Implementing a polar coordinate printer providing a faster printing method and introduces new printing algorithms, increasing printing efficiency. This cylindrical 3D printer applies a circular-moving baseplate and a heating printer head (extruder) moving radially and vertically. A processor accepts pre-processed polar data points and transpose the appropriate programmed printing commands to the motors units. The extruder also has a control loop, keeping the temperature inside the desired range. This prototype prints using polylactic acid (PLA) filament. Additionally, a display screen updates every second the: printing time, extruder temperature, and status notifications

Maintainability index for buildings

Thesis (B.Sc)--University of Hong Kong, 2008.Includes bibliographical references (p. 133-145).published_or_final_versio

Lanthorn, vol. 48, no. 29, December 2, 2013

Lanthorn is Grand Valley State\u27s student newspaper, published from 1968 to the present

Independent- Nov. 3, 1989

https://neiudc.neiu.edu/independent/1030/thumbnail.jp

Receipts and expenditures of the town of Auburn for the year ending February 15, 1909 together with the report of the town and school officers.

This is an annual report containing vital statistics for a town/city in the state of New Hampshire

1987 - 1988 : Bolton Street College of Technology Prospectus

Prospectus for Bolton Street College of Technology for the session 1987 -88. Contains calendar, guide to courses, general informationand regulations, course fees, list of courses, examining bodies and academic staff

Development of carbon nanotube-based bioelectronic sensors using receptor proteins and peptides for to water and food quality assessment and disease diagnosis

학위논문 (박사)-- 서울대학교 대학원 : 협동과정 바이오엔지니어링전공, 2016. 8. 박태현.Bioelectronic sensors that combine biomolecules as primary recognition elements and electronics as signal transducers have been extensively developed.1, 2 Biologically-inspired technologies for detection of target molecules increased the functionality of sensors with the nature system. Nanomaterial-based electronic devices rapidly amplify biological signals and convert into intuitive forms.3, 4, 5 Furthermore, many parts of living organisms are operated by action potentials so that they can be applied to the sensing mechanism of biolectronic sensors. Recently, various nanomaterials, such as carbon nanotube (CNT), graphene, nanoparticle, nanofiber, nanowire and nanofilm have been used in the fabrication of the bioelectronic sensors. Such nanomaterials were functionalized with biomolecules, such as DNA, protein, peptide, and cell for the specific binding. Advantages of using bioelectronic sensors are low manufacturing cost, small size, fast response, high sensitivity and selectivity. The objective of this research is to develop a CNT-based bioelectronic sensor using receptor proteins and peptides for various applications. Receptors receive external stimuli from out of the cell membrane and activate signal transduction pathways.6 The Receptors play important functions in the human body such as sensory transduction, cellular communication, neural transmission and hormonal signaling.7 In this study, receptor proteins were produced in forms of cell-derived nanovesicles, purified proteins and synthetic peptides. Their biological signals were monitored by The CNT-based transducers and applied to environmental monitoring, disease diagnosis and food quality assessment. For the deorphanization of olfactory receptors, artificial olfactory cells were constructed using heterologous reporter gene systems.8 Specific receptors were screened for detection of potential biomarkers and evaluation of cell surface expression was performed. The cell-derived nanovesicles were constructed for the scalable signal transduction to the CNTs.9 A CNT-based field-effect transistor (FET) was fabricated via the conventional photolithography process.10 CNT channels were functionalized with nanovecsicles and the electrical properties of the CNT-FET were also maintained after the immobilization of nanovesicles. The nanovesicle-based bioelectronic sensor detected target biomarkers with high sensitivity and selectivity. The bioelectronic sensor using receptors presented simple rapid analytical methods for water quality monitoring and diabetes diagnosis. Developing a multiplexed platform and miniaturizing sensing device is necessary for practical applications of bioelectronic sensors. Four kinds of receptor proteins were expressed in Escherichia coli, purified and refolded with the detergent micelle methods. The proteins were immobilized onto the multi-type CNT channels which designed for simultaneous detection of the target molecules. A current monitoring device was customized for the multi-channel CNT-FET and operated by a laptop computer. Complex pattern recognitions of various molecules were available without any interference from non-target molecules. The simple portable bioelectronic device was suitable for efficient assessment of food quality and is expected to be used as a rapid on-site sensing platform. Receptor protein-derived peptides were synthesized based on the sequence of ligand binding sites and applied to the development of the bioelectronic sensor. The peptides do not require cell membrane-like environments so that they were easily stored.11 Furthermore, The synthesized peptides could directly immobilized onto the CNTs using the pi-pi interaction by simple modifications with additional phenylalanine residues.12, 13 The peptide-based bioelectronic sensor detected a serum biomarker of nerumyelitis optica with high specificity. Furthermore, the sensitivity was much higher so that the early-phase detection was available. In this research, bioelectronic sensor using carbon nanotubes and various types of receptors such as nanovesicles, proteins and peptides were developed for simple and rapid detection of the biomarkers. The developed sensors applied for the environmental monitoring, disease diagnosis and food quality assessment.Chapter 1. Research background and objective 1 Chapter 2. Literature review 5 2.1 Concept of bioelectronic sensor 6 2.2 Biomolecules as primary recognition elements 7 2.3 Nanomaterials as secondary signal transducers 9 2.4 Bioelectronic sensors using receptor proteins 12 Chapter 3. Experimental procedures 16 3.1 Gene cloning 17 3.2 Production of receptor proteins 17 3.3 Characterization of receptor proteins 19 3.4 Construction of in vitro disease models 21 3.5 Fabrication of bioelectronic sensor 23 3.6 Electrical measurement 26 Chapter 4. Development of bioelectronic sensor using human olfactory nanovesicles for the detection of odor compounds in water pollution 27 4.1 Introduction 28 4.2 Screening of human olfactory receptors 29 4.3 Characterization of olfactory nanovesicles 32 4.4 Construction of nanovesicle-based bioelectronic nose 34 4.5 Detection of GSM and MIB using bioelectronic nose 36 4.6 Detection of GSM and MIB from water samples 42 4.7 Conclusions 44 Chapter 5. Development of bioelectronic sensor using human olfactory nanovesicles for the detection of VOCs from in vitro diabetic models 45 5.1 Introduction 46 5.2 Construction of in vitro diabetic models 47 5.3 Screening of human olfactory receptors 50 5.4 Construction of nanovesicle-based bioelectronic nose 52 5.5 Detection of 17ODYA using bioelectronic nose 55 5.6 Conclusions 57 Chapter 6. Development of portable and multiplexed bioelectronic sensor using human olfactory and taste receptor proteins for the assessment of food quality 58 6.1 Introduction 59 6.2 Production of human sensory receptors 60 6.3 Fabrication of multi-channel bioelectronic sensor 63 6.4 Detection of odor and taste molecules using bioelectronic sensor 69 6.5 Assessment of food freshness using biotelectronic senso 74 6.6 Conclusions 77 Chapter 7. Development of peptide-based bioelectronic sensor using extracellular loops of aquaporin for the diagnosis of neuromyelitis optica 78 7.1 Introduction 79 7.2 Construction of peptide-based bioelectronic sensor 80 7.3 Electrical characteristics of peptide-based bioelectronic sensor 83 7.4 Detection of AQP4 antibody using bioelectronic sensor 85 7.5 Detection of AQP4 antibody in human serum using bioelectronic sensor 87 7.6 Conclusions 89 Chapter 8. Overall discussion and further suggestions 90 Bibliography 95 Abstract 116Docto

Building a Secure Intranet

This thesis will explain the vulnerabilities of computers in a networking environment and demonstrate proper procedures for building a secure Intranet. The Internet is built around the concept of open communication. Data is shared around the globe just as easily as it is from one office or cubical to the next. Corporations are skeptical about putting company data on such a public transport mechanism as the Internet, but the tools used on the Internet are exciting and everyone wants to use them. Out of a desire for the best of both worlds, the Intranet was born. An intranet that has no connection to the Internet can safely make a significant amount of company data available to employees, but when hosts are connected to the Internet, things change. Each application on the Internet comes with a threat to a company\u27s data. More office managers would probably install and use an Intranet if they understood them better and trusted them more. The purpose of his paper is to educate the non-technical manager in the subject of Internet applications and security mechanisms so that he or she can make an informed decision about installing an Intranet. There is so much software available for building and securing a Web site that many feel overwhelmed at the prospect of getting started. The goal will be to define the terms and acronyms used in this technology, and to evaluate the services and software available for building a secure Intranet. Securing a Web site requires some knowledge of TCP/IP, routers, firewalls and data encryption. These subjects will be covered at an introductory level with the goal of enabling the reader to understand the issues involved. The work will terminate in a project that builds an Intranet that shares data with a selective audience while securing it from others. The hardware and software configuration will be documented as a sample that can be duplicated in any office environment. The Web site will be built using some HTML coding to demonstrate the complexity of the language and some high-level software that demonstrates the value of these new tools. Two security specialists evaluated the project. They agreed that an Intranet built with the specifications in the project would be functional and secure

Automated IT Service Fault Diagnosis Based on Event Correlation Techniques

In the previous years a paradigm shift in the area of IT service management could be witnessed. IT management does not only deal with the network, end systems, or applications anymore, but is more and more concerned with IT services. This is caused by the need of organizations to monitor the efficiency of internal IT departments and to have the possibility to subscribe IT services from external providers. This trend has raised new challenges in the area of IT service management, especially with respect to service level agreements laying down the quality of service to be guaranteed by a service provider. Fault management is also facing new challenges which are related to ensuring the compliance to these service level agreements. For example, a high utilization of network links in the infrastructure can imply a delay increase in the delivery of services with respect to agreed time constraints. Such relationships have to be detected and treated in a service-oriented fault diagnosis which therefore does not deal with faults in a narrow sense, but with service quality degradations. This thesis aims at providing a concept for service fault diagnosis which is an important part of IT service fault management. At first, a motivation of the need of further examinations regarding this issue is given which is based on the analysis of services offered by a large IT service provider. A generalization of the scenario forms the basis for the specification of requirements which are used for a review of related research work and commercial products. Even though some solutions for particular challenges have already been provided, a general approach for service fault diagnosis is still missing. For addressing this issue, a framework is presented in the main part of this thesis using an event correlation component as its central part. Event correlation techniques which have been successfully applied to fault management in the area of network and systems management are adapted and extended accordingly. Guidelines for the application of the framework to a given scenario are provided afterwards. For showing their feasibility in a real world scenario, they are used for both example services referenced earlier