Promising Metabolite Profiles in the Plasma and CSF of Early Clinical Parkinson's Disease

Parkinson's disease (PD) shows high heterogeneity with regard to the underlying molecular pathogenesis involving multiple pathways and mechanisms. Diagnosis is still challenging and rests entirely on clinical features. Thus, there is an urgent need for robust diagnostic biofluid markers. Untargeted metabolomics allows establishing low-molecular compound biomarkers in a wide range of complex diseases by the measurement of various molecular classes in biofluids such as blood plasma, serum, and cerebrospinal fluid (CSF). Here, we applied untargeted high-resolution mass spectrometry to determine plasma and CSF metabolite profiles. We semiquantitatively determined small-molecule levels (≤1.5 kDa) in the plasma and CSF from early PD patients (disease duration 0-4 years; n = 80 and 40, respectively), and sex- and age-matched controls (n = 76 and 38, respectively). We performed statistical analyses utilizing partial least square and random forest analysis with a 70/30 training and testing split approach, leading to the identification of 20 promising plasma and 14 CSF metabolites. These metabolites differentiated the test set with an AUC of 0.8 (plasma) and 0.9 (CSF). Characteristics of the metabolites indicate perturbations in the glycerophospholipid, sphingolipid, and amino acid metabolism in PD, which underscores the high power of metabolomic approaches. Further studies will enable to develop a potential metabolite-based biomarker panel specific for PD

A model for assessing procurement irregularities in decision making process at the tendering stage of construction projects

The public procurement in Malaysia has always been regulated by a comprehensive procedure of tender preparation, evaluation and award. Though these measures are meant to insulate unwarranted behaviors or biased decisions of the procurement officers, the public procurement is still plagued with recurring irregularities. Therefore, this study aimed to review and investigate the factors causing irregularities in the current contractor selection and award process. In addition, a conceptual model for improving the procurement decision making process has been developed based on the notion of bounded rationality. In the context of a procurement committee, the individuals were not only influenced by their cognitive limitation, they are also susceptible to irrational group behavior, namely groupthink. The compound of both influences has substantially undermined the deliberation process and hence resulted irregularities in procurement decisions. This research employed quantitative approach and was participated by 289 procurement officers from Malaysian local authorities. Partial Least Square - Structural Equation Modelling (PLS-SEM) statistical analysis technique was employed to test the model. The model confirmed that three antecedents namely accountability, prior knowledge and work experience directly impact the procedural rationality. Whereas, two antecedents namely group insulation and group cohesiveness were directly related to groupthink. Besides, procedural rationality was confirmed to mitigate groupthink effect, whereas groupthink induced defective decision making. In addition, both procedural rationality and defective decision making were found to be associated with procurement decision irregularities. The model was validated for its capability to detect the likelihood of irregularities decisions in the public procurement context

Understanding the Association of Breastfeeding and Food Insecurity on Brain Function in Early Childhood

Introduction: The present study aims to understand how the absence of food security and breastfeeding in children at one year of age, which can be considered as adverse childhood experiences, may be associated with brain function as measured by the relative and absolute power spectral density of four frequency bands of brain waves (theta, alpha, beta, and gamma) among a sample of infants from low-socioeconomic (SES) backgrounds at age 12 months old. Methods: A cross-sectional survey was used by the parent study, Baby’s First Years (BFY), to collect quantitative data to understand the associations between breastfeeding, food insecurity, and brain function in a sample of 243 low-SES mothers and their infants at age 12 months old. Breastfeeding was measured as ever breastfed, to understand if a mother had ever initiated breastfeeding of their infant, and breastfeeding duration, measured in months. Household food insecurity (HFI) was measured using the U.S. Household Food Security Survey Module Short Form devised by the USDA. Electroencephalography (EEG) data was collected to assess brain function. Data Analysis: Data was analyzed to determine associations between being ever breastfed, breastfeeding duration, and the presence of HFI and EEG measured relative and absolute theta, alpha, beta, and gamma power in infants at 12 months of age using multiple linear regression (MLR) models based on ordinary least squares (OLS). Results: 77% (n=187) of mothers reported breastfeeding their child at least one time. The mean breastfeeding duration (including the mothers that never breastfed) was 3.6 months (SD=4.12). 27.6% (n=67) of mothers were found to be food insecure. Ever breastfeeding an infant during the first year of life was found to be associated with higher absolute theta power (p<0.05), and higher relative and absolute alpha power (p<0.01). Breastfeeding duration was not found to be associated with relative and absolute theta, alpha, beta or gamma power. Finally, the presence of food insecurity was not found to be associated with relative and absolute theta, alpha, beta or gamma power. Discussion: Differences in brain function may be adaptive for children experiencing adversity because of their lower SES, amongst other factors (Ellis et al., 2020). Ever breastfeeding an infant was associated with higher absolute theta power, which was an unexpected finding. However, relative theta power was not associated with ever breastfeeding, and therefore this finding must cautiously be interpreted. Ever breastfeeding an infant was associated with higher relative and absolute alpha power. It is possible that the increases in relative and absolute alpha power within the sample of infants who were ever breastfed are in part due to the emotional connection that breastfeeding elicits and the characteristics of mothers that decide to initiate breastfeeding as compared to those that do not initiate breastfeeding. This research demonstrates significant associations between ever breastfeeding an infant with brain function in a population of infants from diverse, low SES backgrounds. In contextualizing these changes in brain function as plausible adaptations that infants are developing due to their experiences, an opportunity exists to further explore these associations with brain function to understand the skills that low SES infants are developing during the first year of life