Chemical analysis and aqueous solution properties of Charged Amphiphilic Block Copolymers PBA-b-PAA synthesized by MADIX

We have linked the structural and dynamic properties in aqueous solution of amphiphilic charged diblock copolymers poly(butyl acrylate)-b-poly(acrylic acid), PBA-b-PAA, synthesized by controlled radical polymerization, with the physico-chemical characteristics of the samples. Despite product imperfections, the samples self-assemble in melt and aqueous solutions as predicted by monodisperse microphase separation theory. However, the PBA core are abnormally large; the swelling of PBA cores is not due to AA (the Flory parameter chiPBA/PAA, determined at 0.25, means strong segregation), but to h-PBA homopolymers (content determined by Liquid Chromatography at the Point of Exclusion and Adsorption Transition LC-PEAT). Beside the dominant population of micelles detected by scattering experiments, capillary electrophoresis CE analysis permitted detection of two other populations, one of h-PAA, and the other of free PBA-b-PAA chains, that have very short PBA blocks and never self-assemble. Despite the presence of these free unimers, the self-assembly in solution was found out of equilibrium: the aggregation state is history dependant and no unimer exchange between micelles occurs over months (time-evolution SANS). The high PBA/water interfacial tension, measured at 20 mN/m, prohibits unimer exchange between micelles. PBA-b-PAA solution systems are neither at thermal equilibrium nor completely frozen systems: internal fractionation of individual aggregates can occur.Comment: 32 pages, 16 figures and 4 tables submitted to Journal of Interface and Colloidal Scienc

Ambiguity, Weakness, and Regularity in Probabilistic B\"uchi Automata

Probabilistic B\"uchi automata are a natural generalization of PFA to infinite words, but have been studied in-depth only rather recently and many interesting questions are still open. PBA are known to accept, in general, a class of languages that goes beyond the regular languages. In this work we extend the known classes of restricted PBA which are still regular, strongly relying on notions concerning ambiguity in classical omega-automata. Furthermore, we investigate the expressivity of the not yet considered but natural class of weak PBA, and we also show that the regularity problem for weak PBA is undecidable

2D to 3D ambience upmixing based on perceptual band allocation

3D multichannel audio systems employ additional elevated loudspeakers in order to provide listeners with a vertical dimension to their auditory experience. Listening tests were conducted to evaluate the feasibility of a novel vertical upmixing technique called “perceptual band allocation (PBA),” which is based on a psychoacoustic principle of vertical sound localization, the “pitch height” effect. The practical feasibility of the method was investigated using 4-channel ambience signals recorded in a reverberant concert hall using the Hamasaki-Square microphone technique. Results showed that the PBA-upmixed 3D stimuli were significantly stronger than or similar to 9-channel 3D stimuli in 3D listener-envelopment (LEV), depending on the sound source and the crossover frequency of PBA. They also significantly produced greater 3D LEV than the 7-channel 3D stimuli. For the preference tests, the PBA stimuli were significantly preferred over the original 9-channel stimuli

Electromagnetic Antenna And Transmission Line Utilizing Photonic Bandgap Material

A photonic bandgap antenna (PBA) (10') utilizes a periodic bandgap material (PBM), which is essentially a dielectric, to transmit, receive, or communicate electromagnetic radiation encoded with information. Further, a photonic bandgap transmission line (PBTL) (10") can also be constructed with the PBM. Because the PBA (10') and PBTL (10") do not utilize metal, the PBA (10') and PBTL (10") can be used in harsh environments, such as those characterized by high temperature and/or high pressure, and can be easily built into a dielectric structure such as a building wall or roof. Further, the PBA (10') and PBTL (10") inhibit scattering by incident electromagnetic radiation at frequencies outside those electromagnetic frequencies in the bandgap range associated with the PBM.Georgia Tech Research Corporatio

Novel elucidation and treatment of pancreatic chronic graft-versus-host disease in mice

Chronic graft-versus-host disease (cGVHD) is a severe complication of allogeneic haematopoietic stem cell transplantation. There is a growing understanding of cGVHD, and several effective therapies for cGVHD have been reported. However, pancreatic cGVHD is a potentially untapped study field. Our thought-provoking study using a mouse model of cGVHD suggested that the pancreas could be impaired by cGVHD-induced inflammation and fibrosis and that endoplasmic reticulum (ER) stress was augmented in the pancreas affected by cGVHD. These findings urged us to treat pancreatic cGVHD through reduction of ER stress, and we used 4-phenylbutyric acid (PBA) as an ER stress reducer. A series of experiments have indicated that PBA can suppress cGVHD-elicited ER stress in the pancreas and accordingly alleviate pancreatic cGVHD. Furthermore, we focused on a correlation between epithelial to mesenchymal transition (EMT) and fibrosis in the cGVHD-affected pancreas, because EMT was conceivably implicated in various fibrosis-associated diseases. Our investigation has suggested that the expression of EMT markers was increased in the cGVHD-disordered pancreas and that it could be reduced by PBA. Taken together, we have provided a clue to elucidate the pathogenic process of pancreatic cGVHD and created a potentially effective treatment of this disease using the ER stress alleviator PBA

Stream Aggregation Through Order Sampling

This is paper introduces a new single-pass reservoir weighted-sampling stream aggregation algorithm, Priority-Based Aggregation (PBA). While order sampling is a powerful and e cient method for weighted sampling from a stream of uniquely keyed items, there is no current algorithm that realizes the benefits of order sampling in the context of stream aggregation over non-unique keys. A naive approach to order sample regardless of key then aggregate the results is hopelessly inefficient. In distinction, our proposed algorithm uses a single persistent random variable across the lifetime of each key in the cache, and maintains unbiased estimates of the key aggregates that can be queried at any point in the stream. The basic approach can be supplemented with a Sample and Hold pre-sampling stage with a sampling rate adaptation controlled by PBA. This approach represents a considerable reduction in computational complexity compared with the state of the art in adapting Sample and Hold to operate with a fixed cache size. Concerning statistical properties, we prove that PBA provides unbiased estimates of the true aggregates. We analyze the computational complexity of PBA and its variants, and provide a detailed evaluation of its accuracy on synthetic and trace data. Weighted relative error is reduced by 40% to 65% at sampling rates of 5% to 17%, relative to Adaptive Sample and Hold; there is also substantial improvement for rank queriesComment: 10 page

Expression of KOC, S100P, mesothelin and MUC1 in pancreatico-biliary adenocarcinomas: development and utility of a potential diagnostic immunohistochemistry panel

<b>Background</b> Pancreatico-biliary adenocarcinomas (PBA) have a poor prognosis. Diagnosis is usually achieved by imaging and/or endoscopy with confirmatory cytology. Cytological interpretation can be difficult especially in the setting of chronic pancreatitis/cholangitis. Immunohistochemistry (IHC) biomarkers could act as an adjunct to cytology to improve the diagnosis. Thus, we performed a meta-analysis and selected KOC, S100P, mesothelin and MUC1 for further validation in PBA resection specimens.<p></p> <b>Methods</b> Tissue microarrays containing tumour and normal cores in a ratio of 3:2, from 99 surgically resected PBA patients, were used for IHC. IHC was performed on an automated platform using antibodies against KOC, S100P, mesothelin and MUC1. Tissue cores were scored for staining intensity and proportion of tissue stained using a Histoscore method (range, 0–300). Sensitivity and specificity for individual biomarkers, as well as biomarker panels, were determined with different cut-offs for positivity and compared by summary receiver operating characteristic (ROC) curve.<p></p> <b>Results</b> The expression of all four biomarkers was high in PBA versus normal ducts, with a mean Histoscore of 150 vs. 0.4 for KOC, 165 vs. 0.3 for S100P, 115 vs. 0.5 for mesothelin and 200 vs. 14 for MUC1 (p < .0001 for all comparisons). Five cut-offs were carefully chosen for sensitivity/specificity analysis. Four of these cut-offs, namely 5%, 10% or 20% positive cells and Histoscore 20 were identified using ROC curve analysis and the fifth cut-off was moderate-strong staining intensity. Using 20% positive cells as a cut-off achieved higher sensitivity/specificity values: KOC 84%/100%; S100P 83%/100%; mesothelin 88%/92%; and MUC1 89%/63%. Analysis of a panel of KOC, S100P and mesothelin achieved 100% sensitivity and 99% specificity if at least 2 biomarkers were positive for 10% cut-off; and 100% sensitivity and specificity for 20% cut-off.<p></p> <b>Conclusion</b> A biomarker panel of KOC, S100P and mesothelin with at least 2 biomarkers positive was found to be an optimum panel with both 10% and 20% cut-offs in resection specimens from patients with PBA.<p></p&gt