The Influence of Illumination Color on the Subjective Visual Recognition of Biological Specimens

[Background] Visual examination by the naked eye is integral to medical diagnosis and surgery. The illumination in conditioned color is widely used for visual inspection in the industry but has not been introduced to the biomedical context. The color that can enhance the visual recognition of individual tissues is still unknown. Therefore, we carried out a visual recognition experiment on biological specimens to determine the subjective preference for illumination color based on questionnaires. [Methods] Twenty healthy subjects were asked to compare the visual recognizability of several rat tissues between the illuminations in test colors and white. The rats were anesthetized, and the femoral vein and abdominal cavity were exposed. Seven tissues were selected for a visual recognition test. Illumination was generated using a multi-color LED light. The subjects observed the tissues under the illuminations of white and one of the test colors alternately and reported which illumination is suitable for visual recognition using a questionnaire. [Results] The analysis of the questionnaires showed that the blue test color was more effective than white illumination in the visual recognition of fine structures such as the branching of blood vessels and nerves, and red illumination disturbed the visual recognizability of the same tissues. On the other hand, the red but not the blue illumination improved the visual recognizability of the vein beneath the intact skin. As to the recognition of individual tissues in the abdominal cavity, the white illumination gave a better visual recognizability compared to every other test color. [Conclusion] This study shows that the illumination color influences the visual recognition of biological specimens and the adequate color for the visual recognition of specific tissue parts is distinct among biological specimens. Using the lighting system to make fine adjustments to the illumination color may be useful in medical diagnosis and surgery

High-speed scanless entire bandwidth mid-infrared chemical imaging

Mid-infrared spectroscopy probes molecular vibrations to identify chemical species and functional groups. Therefore, mid-infrared hyperspectral imaging is one of the most powerful and promising candidates for chemical imaging using optical methods. Yet high-speed and entire bandwidth mid-infrared hyperspectral imaging has not been realized. Here we report a mid-infrared hyperspectral chemical imaging technique that uses chirped pulse upconversion of sub-cycle pulses at the image plane. This technique offers a lateral resolution of 15 $\mu$m, and the field of view is adjustable between 800 $\mu$m $\times$ 600 $\mu$m to 12 mm $\times$ 9 mm. The hyperspectral imaging produces a 640 $\times$ 480 pixel image in 8 s, which covers a spectral range of 640-3015 cm$^{-1}$, comprising 1069 wavelength points and offering a wavenumber resolution of 2.6-3.7 cm$^{-1}$. For discrete frequency mid-infrared imaging, the measurement speed reaches a frame rate of 5 kHz, the repetition rate of the laser. As a demonstration, we effectively identified and mapped different components in a microfluidic device, plant cell, and mouse embryo section. The great capacity and latent force of this technique in chemical imaging promise to be applied to many fields such as chemical analysis, biology, and medicine.Comment: 22 pages, 10 figure

New Trends in 3D Printing

A quarter century period of the 3D printing technology development affords ground for speaking about new realities or the formation of a new technological system of digital manufacture and partnership. The up-to-date 3D printing is at the top of its own overrated expectations. So the development of scalable, high-speed methods of the material 3D printing aimed to increase the productivity and operating volume of the 3D printing machines requires new original decisions. It is necessary to study the 3D printing applicability for manufacturing of the materials with multilevel hierarchical functionality on nano-, micro- and meso-scales that can find applications for medical, aerospace and/or automotive industries. Some of the above-mentioned problems and new trends are considered in this book

Innovative strategies for the development of biopolymer-based materials with suitable properties for biomedical and pharmaceutical applications

Nell'ambito dei biomateriali, i biomateriali polimerici hanno destato particolare interesse per il loro potenziale di applicazione in diversi ambiti biomedici e farmaceutici. Tra questi, i film polimeri sottili e gli idrogel si sono venuti a contraddistinguere come ottimi candidati ai fini della realizzazione di sistemi atti alla veicolazione di agenti terapeutici, medicazioni innovative volte a favorire il processo di guarigione delle ferite, nonché supporti in grado di promuovere la crescita e proliferazione cellulare. Nonostante ciò, tali materiali biopolimerici sono ancora affetti da importanti limitazioni che vanno ad ostacolare fortemente il loro effettivo utilizzo. Sulla base di ciò, il presente lavoro di ricerca si è prefissato lo scopo di investigare alcune delle problematiche principali che possono essere riscontrate durante lo sviluppo di tali matrici polimeriche, al fine di proporre delle strategie alternative valide impiegabili per il superamento di questi ostacoli e volte ad implementare l'applicabilità dei film sottili e degli idrogel negli ambiti biomedici e farmaceutici considerati

Advanced Materials for Oral Application

This book consists of one editorial, 12 original research articles and two review papers from scientists across the world, with expertise in materials for dental application. The main subjects covered are: biomaterials and techniques for oral tissue engineering and regeneration; biomaterials for surgical reconstruction; CAD/CAM technologies and dedicated materials; novel restorative and endodontic materials and instruments

Advances in Food, Bioproducts and Natural Byproducts for a Sustainable Future: From Conventional to Innovative Processes

The world population is expected to reach almost 10,000 million in 2050, so, it is necessary to use our resources efficiently: to produce more food using less land and pollute less, to optimize the production and use of biomass from diversified resources. For this, new approaches and processes, with special emphasis from a biotechnological perspective, may need to be implemented to move towards a circular model that will confer environmental sustainability. In this book, the analysis and optimization of some examples of food and bioproduct processes, as well as development of innovative and emerging food and byproducts processing methods, are considered. Valorization, bioprocessing, and biorefining of food-industry-based streams, the role of industrial microorganisms, the isolation of high added-value compounds, applications of the resulting bio-based chemicals in food manufacturing, novel food formulations, among others, are addressed

Advances in Food, Bioproducts and Natural Byproducts for a Sustainable Future: From Conventional to Innovative Processes

The world population is expected to reach almost 10,000 million in 2050, which entails the need to focus on sustainability and its three pillars: the economy, the environment, and society. Within this context, it is necessary to use our resources efficiently; for instance, we will need to produce much more food using less land and while polluting less to optimize the production of biomass from diversified resources, along with its subsequent conversion, fractionation, and processing. To achieve this, new approaches and processes, with special emphasis from a biotechnological perspective, may need to be implemented to move towards a circular model that will confer environmental sustainability. Global projections of food losses constitute an abundant pool of complex carbohydrates, proteins, lipids, and functional compounds. Hence, the deployment of food waste streams as raw materials will encompass the formulation of added-value products that will be ideally reintroduced in the food supply chain to close the loop. Therefore, the analysis and optimization of any food and bioproduct process, as well as the development of innovative and emerging food and by-product processing methods, are important as a necessity for the sustainable transition to a bioeconomy era. The valorization, bioprocessing, and biorefining of food-industry-based streams, the role of industrial microorganisms, the isolation of high-added-value compounds, applications of the resulting bio-based chemicals in food manufacturing, novel food formulations, economic policies for food waste management, along with sustainability or techno-economic assessment of processing methods constitute subject areas that need to be addressed. More specifically, bioprocess design to valorize food-industry waste and by-product streams should be initiated by characterizing the composition of the onset raw material with the aim of identifying the target end-products, whereas the generation of multiple high-added-value products is a prerequisite for cost-effective processes to establish economic sustainability. On top of that, the feasibility of innovative processes could be sustained by encompassing food applications, driven by the constantly emerging consumers’ demand for functional foods and beverages with enhanced nutritional value. Equally, a growing awareness for bio-based and natural food components is being developed, thereby imposing challenges on the substitution of chemically derived ingredients with their natural counterparts