Wnt activation downregulates olfactomedin-1 in Fallopian tubal epithelial cells:a microenvironment predisposed to tubal ectopic pregnancy

Ectopic pregnancy (EP) occurs when the embryo fails to transit to the uterus and attach to the luminal epithelium of the Fallopian tube (FT). Tubal EP is a common gynecological emergency and more than 95% of EP occurs in the ampullary region of the FT. In humans, Wnt activation and downregulation of olfactomedin-1 (Olfm-1) occur in the receptive endometrium and coincided with embryo implantation in vivo. Whether similar molecular changes happen in the FT leading to EP remains unclear. We hypothesized that activation of Wnt signaling downregulates Olfm-1 expression predisposes to EP. We investigated the spatiotemporal expression of Olfm-1 in FT from non-pregnant women and women with EP, and used a novel trophoblastic spheroid (embryo surrogate)-FT epithelial cell co-culture model (JAr and OE-E6/E7 cells) to study the role of Olfm-1 on spheroid attachment. Olfm-1 mRNA expression in the ampullary region of non-pregnant FT was higher (P0.05) in the follicular phase than in the luteal phase. Ampullary tubal Olfm-1 expression was lower in FT from women with EP compared to normal controls at the luteal phase (histological scoring (H-SCORE)1.30.2 vs 2.40.5; P0.05). Treatment of OE-E6/E7 with recombinant Olfm-1 (0.2-5 g/ml) suppressed spheroid attachment to OE-E6/E7 cells, while activation of Wnt-signaling pathway by Wnt3a or LiCl reduced endogenous Olfm-1 expression and increased spheroid attachment. Conversely, suppression of Olfm-1 expression by RNAi increased spheroid attachment to OE-E6/E7 cells. Taken together, Wnt activation suppresses Olfm-1 expression, and this may predispose a favorable microenvironment of the retained embryo in the FT, leading to EP in humans. © 2012 USCAP, Inc All rights reserved.link_to_OA_fulltex

Hormonal regulation of endometrial olfactomedin expression and its suppressive effect on spheroid attachment onto endometrial epithelial cells

Background Olfactomedin (Olfm) is a member of a diverse group of extracellular matrix proteins important for neuronal growth. Recent microarray studies identified Olfm as one of the down-regulated transcripts in receptive endometrium at the time of embryo attachment and implantation. However, the underlying molecular mechanisms that govern Olfm expression and its effect on embryo attachment and implantation remain unknown. Methods The expression of Olfm in the human endometrium was investigated by real-time PCR, western blotting and immunohistochemistry on human endometrial biopsies from natural and ovarian stimulated cycles. To investigate the function of Olfm in trophoblastendometrial cell attachment, an in vitro spheroid-endometrial cell co-culture study was performed. Results Human endometrial Olfactomedin-1 and -2(Olfm-1 and -2) transcripts decreased significantly from the proliferative to the secretory phases of the menstrual cycle. Olfm protein was strongly expressed in the luminal and glandular epithelium and moderately in the stromal cells of human endometria. Ovarian stimulation significantly decreased (P < 0.05) the expression of endometrial Olfm-1 and -2 transcripts in patients receiving IVF treatment when compared with those in the natural cycle. Importantly, recombinant Olfm-1 suppressed JAr spheroid attachment onto Ishikawa cells and this was not associated with changes of β-catenin and E-cadherin expression in trophoblast and endometrial cells. Conclusions Decreased expression of Olfm during the receptive phase of the endometrium may allow successful trophoblast attachment for implantation. © 2010 The Author.postprin

Olfactomedin1 (Olfm1) in fallopian tube may modulate tubal ectopic pregnancy in humans: evidence from Immunohistochemistry and an in vitro coculture model

Conference Theme: The Intersection Between Genetics, Genomics, and Reproductive BiologyOlfactomedins are secretary glycoprotein constituted in the extracellular matrix (ECM) of various cell types. Recent studies suggested that Olfm-1 is down-regulated during the window of implantation (WOI) in the human endometrium and up-regulated in pathological condition like endometriosis and recurrent spontaneous abortions. Ectopic pregnancy is a gynaecological emergency and fertility threatening phenomenon which occurs in 1-2% of normal pregnancies and shows an increasing trend. Yet, tubal ectopic pregnancy accounts for more than 98% of the cases ...postprintThe 43rd Annual Meeting of the Society for the Study of Preproduction (SSR), Milwaukee, WI., 30 July-3 August 2010. In Biology of Reproduction, 2010, v. 83 n. Meeting abstracts, p. 27-28, abstract no. 13

Opacity Light Fields: Interactive Rendering of Surface Light Fields with View-Dependent Opacity

We present new hardware-accelerated techniques for rendering surface light fields with opacity hulls that allow for interactive visualization of objects that have complex reflectance properties and elaborate geometrical details. The opacity hull is a shape enclosing the object with view-dependent opacity parameterized onto that shape. We call the combination of opacity hulls and surface light fields the opacity light field. Opacity light fields are ideally suited for rendering of the visually complex objects and scenes obtained with 3D photography. We show how to implement opacity light fields in the framework of three surface light field rendering methods: view-dependent texture mapping, unstructured lumigraph rendering, and light field mapping. The modified algorithms can be effectively supported on modern graphics hardware. Our results show that all three implementations are able to achieve interactive or real-time frame rates.Engineering and Applied Science

PLoS One

Vaginal microbicides hold great promise for the prevention of viral diseases like HIV, but the failure of several microbicide candidates in clinical trials has raised important questions regarding the parameters to be evaluated to determine in vivo efficacy in humans. Clinical trials of the candidate microbicides nonoxynol-9 (N9) and cellulose sulfate revealed an increase in HIV infection, vaginal inflammation, and recruitment of HIV susceptible lymphocytes, highlighting the need to identify biomarkers that can accurately predict microbicide toxicity early in preclinical development and in human trials. We used quantitative proteomics and RT-PCR approaches in mice and rabbits to identify protein changes in vaginal fluid and tissue in response to treatment with N9 or benzalkonium chloride (BZK). We compared changes generated with N9 and BZK treatment to the changes generated in response to tenofovir gel, a candidate microbicide that holds promise as a safe and effective microbicide. Both compounds down regulated mucin 5 subtype B, and peptidoglycan recognition protein 1 in vaginal tissue; however, mucosal brush samples also showed upregulation of plasma proteins fibrinogen, plasminogen, apolipoprotein A-1, and apolipoprotein C-1, which may be a response to the erosive nature of N9 and BZK. Additional proteins down-regulated in vaginal tissue by N9 or BZK treatment include CD166 antigen, olfactomedin-4, and anterior gradient protein 2 homolog. We also observed increases in the expression of C-C chemokines CCL3, CCL5, and CCL7 in response to treatment. There was concordance in expression level changes for several of these proteins using both the mouse and rabbit models. Using a human vaginal epithelial cell line, the expression of mucin 5 subtype B and olfactomedin-4 were down-regulated in response to N9, suggesting these markers could apply to humans. These data identifies new proteins that after further validation could become part of a panel of biomarkers to effectively evaluate microbicide toxicity

Human chorionic gonadotropin stimulates spheroid attachment on fallopian tube epithelial cells through the mitogen-activated protein kinase pathway and down-regulation of olfactomedin-1

OBJECTIVE: To study the effect of human chorionic gonadotropin (hCG) on olfactomedin-1 (Olfm1) expression and spheroid attachment in human fallopian tube epithelial cells in vitro. DESIGN: Experimental study. SETTING: Reproductive biology laboratory. PATIENT(S): Healthy non-pregnant women. INTERVENTION(S): No patient interventions. MAIN OUTCOME MEASURE(S): Luteinizing hormone/chorionic gonadotropin receptor (LHCGR) and Olfm1 expression in fallopian tube epithelium cell line (OE-E6/E7 cells). OE-E6/E7 cells treated with hCG, U0126 extracellular signal-regulated kinase (ERK) inhibitor, or XAV939 Wnt/β-catenin inhibitor were analyzed by Western blotting, real-time polymerase chain reaction, and in vitro spheroid attachment assay. RESULT(S): Human chorionic gonadotropin increased spheroid attachment on OE-E6/E7 cells through down-regulation of Olfm1 and activation of Wnt and mitogen-activated protein kinase (MAPK) signaling pathways. U0126 down-regulated both MAPK and Wnt/β-catenin signaling pathways and up-regulated Olfm1 expression. XAV939 down-regulated only the Wnt/β-catenin signaling pathway but up-regulated Olfm1 expression. CONCLUSION(S): Human chorionic gonadotropin activated both ERK and Wnt/β-catenin signaling pathways and enhanced spheroid attachment on fallopian tube epithelial cells through down-regulation of Olfm1 expression.postprin

Involvement of Romania’s European Integration in the Field of Employment and Efficient Labour Use

The beginning of the article presents the general structure of Romania employed population while joining the European Union. It is proved in figures the extent to which the transition to market economy affected Romania’s workforce, both on sexes and ages and also on socio-professional categories and activity fields. The article’s aim is to show us why the Romanian workforce migrates and which are the dimensions of this migration in the conditions of EU joining. At the same time, it shows us where Romanian migrate, the amount of persons that migrate and the price they pay for this. Finally, it is mentioned the way the workforce employment and usage will evolve during the post joining period, the positive and negative social implications of this complex process and also the forecast solutions for the emigrants coming back to Romania.workforce; workforce migration; the rate of unemployment; the minimum gross salary; the price of emigration.

Olfactomedin‑like 2 A and B (OLFML2A and OLFML2B) expression profile in primates (human and baboon)

Background: The olfactomedin‑like domain (OLFML) is present in at least four families of proteins, including OLFML2A and OLFML2B, which are expressed in adult rat retina cells. However, no expression of their orthologous has ever been reported in human and baboon. Objective: The aim of this study was to investigate the expression of OLFML2A and OLFML2B in ocular tissues of baboons (Papio hamadryas) and humans, as a key to elucidate OLFML function in eye physiology. Methods: OLFML2A and OLFML2B cDNA detection in ocular tissues of these species was performed by RT‑PCR. The amplicons were cloned and sequenced, phylogenetically analyzed and their proteins products were confirmed by immunofluorescence assays. Results: OLFML2A and OLFML2B transcripts were found in human cornea, lens and retina and in baboon cornea, lens, iris and retina. The baboon OLFML2A and OLFML2B ORF sequences have 96% similarity with their human’s orthologous. OLFML2A and OLFML2B evolution fits the hypothesis of purifying selection. Phylogenetic analysis shows clear orthology in OLFML2A genes, while OLFML2B orthology is not clear. Conclusions: Expression of OLFML2A and OLFML2B in human and baboon ocular tissues, including their high simi‑ larity, make the baboon a powerful model to deduce the physiological and/or metabolic function of these proteins in the eye

UA12/2/1 College Heights Herald, Vol. 26, No. 8-Z245

WKU campus newspaper reporting campus, athletic and Bowling Green, Kentucky news. This issue is mis-numbered as #28. Regular features include: Book Marks Hilltopics Society Page Faculty Notes Club News Sports Birds I View Looking Backward Kentucky Building News Alumni Flashes Personals Vets Views Kentucky Building News Articles in this issue: Hilltoppers to Meet TPI in OVC Tournament Tonight Geography Club Reorganized Hawkins, Edith. Final Concert of Season to Feature Nan Merriman Talisman Ball May be May 12 Pasdena Art on Display William Spudnardi Injured in Auto Accident Band to Feature “Symphony in B” Philharmonic to Give Concert Next Month Six to Try for Robinson Medal Stone, Vernon. Should Teacher’s Loyalty Oaths be Required? Faculty Offer Opinions A Team to Remember – editorial Washington’s Birthday Registrar’s Office LIsts 402 on Semester Honor Roll Johanna Maples Wins Contest Former Student Edits Army Paper – Paul Vance UNESCO Shows Interest in Coming Production of “The Petrified Forest” Students Heard on WLBJ, WKCT Western Students Cast in “Light Up in the Sky” Students Attend Ball Given by Eastern ROTC Gene Napper Elected Potter Mayor Librarians Meet for Staff Discussion Four ROTC Students Earn Promotions Mr. & Mrs. L.T. Hardin Are at Fern Creek Roger Ganem Manages Investors Syndicate Smith, Katheryn. Students Define Ideal Professor in Many Ways String Quartet Gives Recital This Week College High Students to Give Play Dr. Hugh Gunderson Named Chairman 12 Students Play in Monthly Recital Cedar House Story Western Blood Donors Listed President, Dean Attending Meet Western Gym Named as Tournament Site Hilltoppers Set 16 Records During Regular Season Play Shaver, Pat. Sports Alley Former Toppers Now Succeed as Professionals Rifle Team Nips Ft. Knox. Harpist Gives Chapel Concert – Christine Carr Dr. Alfred Crabb Heard on WSM Broadcast Miss Justine Lynn Leaves to Study at Columbia Pershing Rifles is Reorganized Burd, Bill. Fullbright Scholar Tells of English Situation Folklorist Will Address Chapel – Seth Thompson Adon Foster Judges Symphony Auditions Fifth Graders Write and Produce Pageant Major Claude McRaven Plans Summer Camp Duty 34 Girls Enroll for Rifle Team Demons Defeat Wildcats in Intramural Basketball Game Dr. Earl Moore Speaks at Civic Program