Culture medium pH is influenced by basal medium, carbohydrate source, gelling agent, activated charcoal, and medium storage method

When four carbohydrates were tested against six commonly cited inorganic basal media, post-autoclave pH was highest for carbohydrate-free and sucrose- containing media, and progressively lower for maltose-, P lucose-, and fructose-containing media, respectively, post-autoclave pH for these media without carbohydrates was related to medium buffering capacity. Addition of gelling agents (10 of 11 tested) increased the post¬autoclave pH of MS medium containing sucrose. Neutralized and acid-washed activated charcoal also increased the post-autoclave pH of liquid and agar- solidified MS medium, and the pH changed further during 8 weeks of storage. Changes in medium pH caused by gelling agents, but not charcoal, could be alleviated by adjusting the pH after their addition but prior to autoclaving

Pemanfaatan Ubi Jalar Putih dan Ubi Jalar Kuning Sebagai Media Alternatif Pertumbuhan Staphylococcus aureus

Nutrient Agar medium is the growth mediums for Staphylococcus aureus that has been tested to grow colonies well. NA has an expensive price, so alternative media from nature-based are needed such as white-fleshed and yellow-fleshed sweet potato which are a source of carbohydrates. This research is post-test only control group design using spread plate method that aims to find the difference in Staphylococcus aureus growth ability in white-fleshed sweet potato medium, yellow-fleshed sweet potato medium, and Nutrient Agar. Staphylococcus aureus suspense was inoculated into each growth medium and then incubated at 37°C for 72 hours. The determination of characteristics and number of colonies of Staphylococcus aureus are made every 24 hours of incubation. The number of colonies of Staphylococcus aureus at 48 hours of incubation was processed using the post-hoc test as an advanced test, the results showed that there were differences in the ability of the growth media to grow Staphylococcus aureus. Yellow-fleshed sweet potato media and NA medium had the same ability, but white-fleshed sweet potato media was better at growing Staphylococcus aureus than yellow-fleshed sweet potato media

Characteristics of human spermatozoa harvested in culture media with and without serum proteins

This study was aimed to determine the efficiency of synthetic protein-free media in spermatozoa washing, preparation and retention of the activity of washed spermatozoa over short periods in vitro. Normozoospermic semen samples (n = 71) were equally apportioned and washed using synthetic protein-free medium (PFM), minimum essential medium + HSA (MEM) or commercial protein-containing medium (CPC). Washed spermatozoa were cultured in vitro using PFM, MEM or CPC media and held for 24 hrs at 4°C, 15°C, 22°C or 37°C. Spermatozoa activity was evaluated at 0 hr, 4 to 7 hrs and 24 hrs post-wash. The effects of PFM on spermatozoa motility, vitality, membrane integrity and DNA fragmentation level were not significantly different from that of MEM and CPC media at 0 hr, 4 to 7 hrs and 24 hrs post-wash in vitro. Synthetic PFM, MEM and CPC retained spermatozoa activity highest when specimen were held at 22°C and it was significantly higher (p 0.05) were noted in spermatozoa DNA fragmentation (SDF) levels when specimen were held at 22°C or 37°C at 4 to 7 hrs and also after 24 hrs post-wash in vitro in all media. The use of synthetic PFM as an alternative to the commercial protein-containing media in human spermatozoa washing and preparation procedure for an efficient and safer (Assisted Reproduction Technology) ART outcome. Spermatozoa activity can be successfully retained at room temperature post-wash over short periods; spermatozoa may lose viability rapidly if held for long hours at 37°C in all media

Regeneration of Senecio x hybridus Hly. (florists\u27cineraria) by somatic embryogenesis

The somatic embryogenic potential of Senecio x hybridus Hyl (Florists\u27 Cineraria) was explored. Somatic embryos were initiated on semi-solid MS basal media amended with 3 mg/l 2,4-D and 1 mg/l BA (induction medium). An inverse relationship existed between sucrose concentration in the induction medium and globular embryo formation when sucrose exceeded 3%. The first and second leaves, from 13-18 and 20 days post-sown seedlings, respectively, were more responsive explants than cotyledons from the same source and sampling times. Somatic embryo development beyond the globular stage was greatly delayed or prevented by remaining on auxin-rich induction medium; therefore several two or three media transfer systems using either 0.5% or 1.0% AC were attempted. The two media system using (1) induction medium and a transfer to (2) MS basal medium with 1.0% AC, but without hormones, caused explant death or decline by 28 days post-inoculation to induction medium. The three media transfer system using (1) induction medium, (2) MS basal medium without hormones, supplemented with 0.5% AC for three days followed by a transfer to (3) MS basal medium without hormones and AC produced mature somatic embryos by 28 days post-inoculation to induction medium; however, embryos germinated precociously. Occasionally, explants that were not transferred from the initial induction medium (controls) developed mature embryos that did not germinate precociously after two-four months. Somatic embryos converted to plants and were grown to maturity: however, most plants were multi-stemmed. Histological examination of mature somatic embryos produced verification of embryo anatomy

Medium practices

In this essay I develop a topic addressed in my book, Film Art Phenomena: the question of medium specificity. Rosalind Krauss's essay 'Art In the Age of the Post-Medium Condition' has catalysed a move away from medium specificity to hybridity. I propose that questions of medium cannot be ignored, since they carry their own history and give rise to specific formal traits and possibilities. The research involves close critical analysis of four moving image works that have not previously been written about: two made with film, and one each with computer and mobile phone. The analyses are conducted by reference to my ideas about how technological peculiarities inform and inflect practice: I see the work's material composition, its form and final meaning as intricately bound up with each other. Film, video and the computer give rise to specific forms of moving image, partly because artists exploit a medium’s peculiarities, and because certain media lend themselves to some methodologies and not others. I do not seek hard distinctions between these media, but discuss them in terms of predispositions. For example, I discuss a 16mm cine film in which the shifting visibility of grain raises ideas around movement and stillness. The aim is to develop a definition of medium specificity, in relation to the moving image, that is not essentialist in the way previous versions were criticised for being, that is, based on ideas of "material substrate" (Wollen). I argue that film is a medium of stages, in contrast to the modern tapeless camcorder, in which all functions of recording, storage, playback and even editing are contained in a single device. Supported by a travel grant, I presented a version of this essay at the International Conference of Experimental Media Congress, Toronto, in April 2011, along with a selection of works: http://www.experimentalcongress.org/full-schedule

Communication Media Technology And Social Harmony Construction In The Era Of Society 5.0 A Critical View

Abstract—The current media communication technology is growing into an inevitability. This medium can make a world as big as an egg, and it can similarly build a harmony based on its will. The study revealed how communication media technologies are able to build a reality of harmony in society. The study employed a qualitative approach with a post-positivistic paradigm. The research took 5 informants and interviewed them. The results of the study, (1) communication media technology became the main key formed society 5.0. (2) The development of social media in various platforms and with all its advantages caused the mass media mind stream fall one by one. (3) Communication media technology into a medium can bring the far and away close, or in other words create harmony that is switched. (4) Communication media technology has an impact on the occurrence of hoaxes that are actually enemies of the state

Findings on Student Use of Social Media at the Collegiate, Undergraduate, and Graduate Levels: Implications for Post-Secondary Educators

In this paper, we present findings on social media use by students at two institutions in three levels of post-secondary programs. We find that students are almost universally using at least one social network, with Facebook as the most popular, and Instagram second. Many respondents are simultaneously active on several social networks. However, few post to any social medium more than once per day. Social media usage levels of students in our survey far exceeded that of the adult Canadian population at large. Changes in student posting habits during the course varied widely with the level of post-secondary program, as did views on the professional applications of social media

Impact of Social Media on Brand Equity and Profitability in Micro, Small and Medium Enterprises in Nigeria

Social media has opened doors of endless opportunities for businesses at little or no cost and over the years social media has gradually been gaining ground in the world. The objective of this study was to assess the impact of social media on brand equity and profitability in micro, small and medium enterprises in Nigeria with particular emphasis on the Federal Capital Abuja. The survey method was adopted for the study and questionnaires were administered as method of primary data collection. The data was analysed by the simple descriptive percentage method and formulated hypotheses tested using Kendall coefficient of concordance method. And the findings revealed that there is need for social media in business interaction in Nigeria, there is significant impact of social media on brand equity and brand equity have impact on profitability in micro, small and medium enterprises in Nigeria and there is also a significant impact of social media on consumer purchase decision. The study among other things recommended that Micro, small and medium enterprises should create a social media plan that fits into their goals and objectives, post on social media platforms regularly, build relationships and be professional in their dealings with consumers, in order to improve on their profitabilit