SPHERE: the exoplanet imager for the Very Large Telescope

Observations of circumstellar environments to look for the direct signal of exoplanets and the scattered light from disks has significant instrumental implications. In the past 15 years, major developments in adaptive optics, coronagraphy, optical manufacturing, wavefront sensing and data processing, together with a consistent global system analysis have enabled a new generation of high-contrast imagers and spectrographs on large ground-based telescopes with much better performance. One of the most productive is the Spectro-Polarimetic High contrast imager for Exoplanets REsearch (SPHERE) designed and built for the ESO Very Large Telescope (VLT) in Chile. SPHERE includes an extreme adaptive optics system, a highly stable common path interface, several types of coronagraphs and three science instruments. Two of them, the Integral Field Spectrograph (IFS) and the Infra-Red Dual-band Imager and Spectrograph (IRDIS), are designed to efficiently cover the near-infrared (NIR) range in a single observation for efficient young planet search. The third one, ZIMPOL, is designed for visible (VIR) polarimetric observation to look for the reflected light of exoplanets and the light scattered by debris disks. This suite of three science instruments enables to study circumstellar environments at unprecedented angular resolution both in the visible and the near-infrared. In this work, we present the complete instrument and its on-sky performance after 4 years of operations at the VLT.Comment: Final version accepted for publication in A&

Quantitative magneto-optical investigation of superconductor/ferromagnet hybrid structures

We present a detailed quantitative magneto-optical imaging study of several superconductor/ferromagnet hybrid structures, including Nb deposited on top of thermomagnetically patterned NdFeB, and permalloy/niobium with erasable and tailored magnetic landscapes imprinted in the permalloy layer. The magneto-optical imaging data is complemented with and compared to scanning Hall probe microscopy measurements. Comprehensive protocols have been developed for calibrating, testing, and converting Faraday rotation data to magnetic field maps. Applied to the acquired data, they reveal the comparatively weaker magnetic response of the superconductor from the background of larger fields and field gradients generated by the magnetic layer.Comment: 21 pages, including 2 pages of supplementary materia

Diattenuation of Brain Tissue and its Impact on 3D Polarized Light Imaging

3D-Polarized Light Imaging (3D-PLI) reconstructs nerve fibers in histological brain sections by measuring their birefringence. This study investigates another effect caused by the optical anisotropy of brain tissue - diattenuation. Based on numerical and experimental studies and a complete analytical description of the optical system, the diattenuation was determined to be below 4 % in rat brain tissue. It was demonstrated that the diattenuation effect has negligible impact on the fiber orientations derived by 3D-PLI. The diattenuation signal, however, was found to highlight different anatomical structures that cannot be distinguished with current imaging techniques, which makes Diattenuation Imaging a promising extension to 3D-PLI.Comment: 32 pages, 15 figure

J Fluorescence

The scope of this paper is to illustrate the need for an improved quality assurance in fluorometry. For this purpose, instrumental sources of error and their influences on the reliability and comparability of fluorescence data are highlighted for frequently used photoluminescence techniques ranging from conventional macro- and microfluorometry over fluorescence microscopy and flow cytometry to microarray technology as well as in vivo fluorescence imaging. Particularly, the need for and requirements on fluorescence standards for the characterization and performance validation of fluorescence instruments, to enhance the comparability of fluorescence data, and to enable quantitative fluorescence analysis are discussed. Special emphasis is dedicated to spectral fluorescence standards and fluorescence intensity standards

High-speed in vitro intensity diffraction tomography

We demonstrate a label-free, scan-free intensity diffraction tomography technique utilizing annular illumination (aIDT) to rapidly characterize large-volume three-dimensional (3-D) refractive index distributions in vitro. By optimally matching the illumination geometry to the microscope pupil, our technique reduces the data requirement by 60 times to achieve high-speed 10-Hz volume rates. Using eight intensity images, we recover volumes of ∼350 μm  ×  100 μm  ×  20  μm, with near diffraction-limited lateral resolution of   ∼  487  nm and axial resolution of   ∼  3.4  μm. The attained large volume rate and high-resolution enable 3-D quantitative phase imaging of complex living biological samples across multiple length scales. We demonstrate aIDT’s capabilities on unicellular diatom microalgae, epithelial buccal cell clusters with native bacteria, and live Caenorhabditis elegans specimens. Within these samples, we recover macroscale cellular structures, subcellular organelles, and dynamic micro-organism tissues with minimal motion artifacts. Quantifying such features has significant utility in oncology, immunology, and cellular pathophysiology, where these morphological features are evaluated for changes in the presence of disease, parasites, and new drug treatments. Finally, we simulate the aIDT system to highlight the accuracy and sensitivity of the proposed technique. aIDT shows promise as a powerful high-speed, label-free computational microscopy approach for applications where natural imaging is required to evaluate environmental effects on a sample in real time.https://arxiv.org/abs/1904.06004Accepted manuscrip

Terahertz dynamic aperture imaging at stand-off distances using a Compressed Sensing protocol

In this text, results of a 0.35 terahertz (THz) dynamic aperture imaging approach are presented. The experiments use an optical modulation approach and a single pixel detector at a stand-off imaging distance of approx 1 meter. The optical modulation creates dynamic apertures of 5cm diameter with approx 2000 individually controllable elements. An optical modulation approach is used here for the first time at a large far-field distance, for the investigation of various test targets in a field-of-view of 8 x 8 cm. The results highlight the versatility of this modulation technique and show that this imaging paradigm is applicable even at large far-field distances. It proves the feasibility of this imaging approach for potential applications like stand-off security imaging or far field THz microscopy.Comment: 9 pages, 13 figure

Coronagraphic Low Order Wave Front Sensor : post-processing sensitivity enhancer for high performance coronagraphs

Detection and characterization of exoplanets by direct imaging requires a coronagraph designed to deliver high contrast at small angular separation. To achieve this, an accurate control of low order aberrations, such as pointing and focus errors, is essential to optimize coronagraphic rejection and avoid the possible confusion between exoplanet light and coronagraphic leaks in the science image. Simulations and laboratory prototyping have shown that a Coronagraphic Low Order Wave-Front Sensor (CLOWFS), using a single defocused image of a reflective focal plane ring, can be used to control tip-tilt to an accuracy of 10^{-3} lambda/D. This paper demonstrates that the data acquired by CLOWFS can also be used in post-processing to calibrate residual coronagraphic leaks from the science image. Using both the CLOWFS camera and the science camera in the system, we quantify the accuracy of the method and its ability to successfully remove light due to low order errors from the science image. We also report the implementation and performance of the CLOWFS on the Subaru Coronagraphic Extreme AO (SCExAO) system and its expected on-sky performance. In the laboratory, with a level of disturbance similar to what is encountered in a post Adaptive Optics beam, CLOWFS post-processing has achieved speckle calibration to 1/300 of the raw speckle level. This is about 40 times better than could be done with an idealized PSF subtraction that does not rely on CLOWFS.Comment: 10 pages, 7 figures, accepted for publication in PAS