ARIGAN: Synthetic Arabidopsis Plants using Generative Adversarial Network

In recent years, there has been an increasing interest in image-based plant phenotyping, applying state-of-the-art machine learning approaches to tackle challenging problems, such as leaf segmentation (a multi-instance problem) and counting. Most of these algorithms need labelled data to learn a model for the task at hand. Despite the recent release of a few plant phenotyping datasets, large annotated plant image datasets for the purpose of training deep learning algorithms are lacking. One common approach to alleviate the lack of training data is dataset augmentation. Herein, we propose an alternative solution to dataset augmentation for plant phenotyping, creating artificial images of plants using generative neural networks. We propose the Arabidopsis Rosette Image Generator (through) Adversarial Network: a deep convolutional network that is able to generate synthetic rosette-shaped plants, inspired by DCGAN (a recent adversarial network model using convolutional layers). Specifically, we trained the network using A1, A2, and A4 of the CVPPP 2017 LCC dataset, containing Arabidopsis Thaliana plants. We show that our model is able to generate realistic 128x128 colour images of plants. We train our network conditioning on leaf count, such that it is possible to generate plants with a given number of leaves suitable, among others, for training regression based models. We propose a new Ax dataset of artificial plants images, obtained by our ARIGAN. We evaluate this new dataset using a state-of-the-art leaf counting algorithm, showing that the testing error is reduced when Ax is used as part of the training data.Comment: 8 pages, 6 figures, 1 table, ICCV CVPPP Workshop 201

DeepWheat: Estimating Phenotypic Traits from Crop Images with Deep Learning

In this paper, we investigate estimating emergence and biomass traits from color images and elevation maps of wheat field plots. We employ a state-of-the-art deconvolutional network for segmentation and convolutional architectures, with residual and Inception-like layers, to estimate traits via high dimensional nonlinear regression. Evaluation was performed on two different species of wheat, grown in field plots for an experimental plant breeding study. Our framework achieves satisfactory performance with mean and standard deviation of absolute difference of 1.05 and 1.40 counts for emergence and 1.45 and 2.05 for biomass estimation. Our results for counting wheat plants from field images are better than the accuracy reported for the similar, but arguably less difficult, task of counting leaves from indoor images of rosette plants. Our results for biomass estimation, even with a very small dataset, improve upon all previously proposed approaches in the literature.Comment: WACV 2018 (Code repository: https://github.com/p2irc/deepwheat_WACV-2018

Object Counting with Deep Learning

This thesis explores various empirical aspects of deep learning or convolutional network based models for efficient object counting. First, we train moderately large convolutional networks on comparatively smaller datasets containing few hundred samples from scratch with conventional image processing based data augmentation. Then, we extend this approach for unconstrained, outdoor images using more advanced architectural concepts. Additionally, we propose an efficient, randomized data augmentation strategy based on sub-regional pixel distribution for low-resolution images. Next, the effectiveness of depth-to-space shuffling of feature elements for efficient segmentation is investigated for simpler problems like binary segmentation -- often required in the counting framework. This depth-to-space operation violates the basic assumption of encoder-decoder type of segmentation architectures. Consequently, it helps to train the encoder model as a sparsely connected graph. Nonetheless, we have found comparable accuracy to that of the standard encoder-decoder architectures with our depth-to-space models. After that, the subtleties regarding the lack of localization information in the conventional scalar count loss for one-look models are illustrated. At this point, without using additional annotations, a possible solution is proposed based on the regulation of a network-generated heatmap in the form of a weak, subsidiary loss. The models trained with this auxiliary loss alongside the conventional loss perform much better compared to their baseline counterparts, both qualitatively and quantitatively. Lastly, the intricacies of tiled prediction for high-resolution images are studied in detail, and a simple and effective trick of eliminating the normalization factor in an existing computational block is demonstrated. All of the approaches employed here are thoroughly benchmarked across multiple heterogeneous datasets for object counting against previous, state-of-the-art approaches

A Segmentation-Guided Deep Learning Framework for Leaf Counting

Deep learning-based methods have recently provided a means to rapidly and effectively extract various plant traits due to their powerful ability to depict a plant image across a variety of species and growth conditions. In this study, we focus on dealing with two fundamental tasks in plant phenotyping, i.e., plant segmentation and leaf counting, and propose a two-steam deep learning framework for segmenting plants and counting leaves with various size and shape from two-dimensional plant images. In the first stream, a multi-scale segmentation model using spatial pyramid is developed to extract leaves with different size and shape, where the fine-grained details of leaves are captured using deep feature extractor. In the second stream, a regression counting model is proposed to estimate the number of leaves without any pre-detection, where an auxiliary binary mask from segmentation stream is introduced to enhance the counting performance by effectively alleviating the influence of complex background. Extensive pot experiments are conducted CVPPP 2017 Leaf Counting Challenge dataset, which contains images of Arabidopsis and tobacco plants. The experimental results demonstrate that the proposed framework achieves a promising performance both in plant segmentation and leaf counting, providing a reference for the automatic analysis of plant phenotypes

Leveraging Image Analysis for High-Throughput Plant Phenotyping

The complex interaction between a genotype and its environment controls the biophysical properties of a plant, manifested in observable traits, i.e., plant’s phenome, which influences resources acquisition, performance, and yield. High-throughput automated image-based plant phenotyping refers to the sensing and quantifying plant traits non-destructively by analyzing images captured at regular intervals and with precision. While phenomic research has drawn significant attention in the last decade, extracting meaningful and reliable numerical phenotypes from plant images especially by considering its individual components, e.g., leaves, stem, fruit, and flower, remains a critical bottleneck to the translation of advances of phenotyping technology into genetic insights due to various challenges including lighting variations, plant rotations, and self-occlusions. The paper provides (1) a framework for plant phenotyping in a multimodal, multi-view, time-lapsed, high-throughput imaging system; (2) a taxonomy of phenotypes that may be derived by image analysis for better understanding of morphological structure and functional processes in plants; (3) a brief discussion on publicly available datasets to encourage algorithm development and uniform comparison with the state-of-the-art methods; (4) an overview of the state-of-the-art image-based high-throughput plant phenotyping methods; and (5) open problems for the advancement of this research field

An Exploration of Deep-Learning Based Phenotypic Analysis to Detect Spike Regions in Field Conditions for UK Bread Wheat

Wheat is one of the major crops in the world, with a global demand expected to reach 850 million tons by 2050 that is clearly outpacing current supply. The continual pressure to sustain wheat yield due to the world’s growing population under fluctuating climate conditions requires breeders to increase yield and yield stability across environments. We are working to integrate deep learning into field-based phenotypic analysis to assist breeders in this endeavour. We have utilised wheat images collected by distributed CropQuant phenotyping workstations deployed for multiyear field experiments of UK bread wheat varieties. Based on these image series, we have developed a deep-learning based analysis pipeline to segment spike regions from complicated backgrounds. As a first step towards robust measurement of key yield traits in the field, we present a promising approach that employ Fully Convolutional Network (FCN) to perform semantic segmentation of images to segment wheat spike regions. We also demonstrate the benefits of transfer learning through the use of parameters obtained from other image datasets. We found that the FCN architecture had achieved a Mean classification Accuracy (MA) >82% on validation data and >76% on test data and Mean Intersection over Union value (MIoU) >73% on validation data and and >64% on test datasets. Through this phenomics research, we trust our attempt is likely to form a sound foundation for extracting key yield-related traits such as spikes per unit area and spikelet number per spike, which can be used to assist yield-focused wheat breeding objectives in near future