R.A.Fisher, design theory, and the Indian connection

Design Theory, a branch of mathematics, was born out of the experimental statistics research of the population geneticist R. A. Fisher and of Indian mathematical statisticians in the 1930s. The field combines elements of combinatorics, finite projective geometries, Latin squares, and a variety of further mathematical structures, brought together in surprising ways. This essay will present these structures and ideas as well as how the field came together, in itself an interesting story.Comment: 11 pages, 3 figure

Playing with the dead:transmedia narratives and the Walking Dead games

This chapter discusses the theory and practice of transmedia narratives within the storyworld created by Robert Kirkman, Tony Moore and Charlie Adlard’s comics series The Walking Dead. It examines key aspects from the comics series and AMC’s adaptive television franchise to consider how both have been utilized and adapted for games. Particular focus will be paid to Telltale Games’ The Walking Dead, Gamagio’s The Walking Dead Assault and Terminal Reality’s The Walking Dead: Survival Instinct. The chapter explains the core concepts of transmedia narratives as they relate to The Walking Dead, places the games in the context of both the comics and television franchise, examines the significance of commercial and grassroot extensions and considers the role gaming and interactive narratives have within rich storyworlds. In examining The Walking Dead as a transmedia property, the authors demonstrate how vast narratives are adopted, modified and transformed in contemporary popular culture

The D/H Ratio in the Interstellar Medium toward the White Dwarf PG0038+199

We determine the D/H ratio in the interstellar medium toward the DO white dwarf PG0038+199 using spectra from the Far Ultraviolet Spectroscopic Explorer (FUSE), with ground-based support from Keck HIRES. We employ curve of growth, apparent optical depth and profile fitting techniques to measure column densities and limits of many other species (H2, NaI, CI, CII, CIII, NI, NII, OI, SiII, PII, SIII, ArI and FeII) which allow us to determine related ratios such as D/O, D/N and the H2 fraction. Our efforts are concentrated on measuring gas-phase D/H, which is key to understanding Galactic chemical evolution and comparing it to predictions from Big Bang nucleosynthesis. We find column densities log N(HI) = 20.41+-0.08, log N(DI)=15.75+-0.08 and log N(H2) = 19.33+-0.04, yielding a molecular hydrogen fraction of 0.14+-0.02 (2 sigma errors), with an excitation temperature of 143+-5K. The high HI column density implies that PG0038+199 lies outside of the Local Bubble; we estimate its distance to be 297 (+164,-104)pc (1 sigma). D/[HI+2H2] toward PG0038+199 is 1.91(+0.52,-0.42) e-5 (2 sigma). There is no evidence of component structure on the scale of Delta v > 8 km/s based on NaI, but there is marginal evidence for structure on smaller scales. The D/H value is high compared to the majority of recent D/H measurements, but consistent with the values for two other measurements at similar distances. D/O is in agreement with other distant measurements. The scatter in D/H values beyond ~100pc remains a challenge for Galactic chemical evolution.Comment: 59 pages, 7 tables, 18 figures (1 standalone), accepted by ApJ v2 minor typos correcte

Human sperm ion channel (dys)function:implications for fertilization

BACKGROUND: Intensive research on sperm ion channels has identified members of several ion channel families in both mouse and human sperm. Gene knock-out studies have unequivocally demonstrated the importance of the calcium and potassium conductances in sperm for fertility. In both species, the calcium current is carried by the highly complex cation channel of sperm (CatSper). In mouse sperm, the potassium current has been conclusively shown to be carried by a channel consisting of the pore forming subunit SLO3 and auxiliary subunit leucine-rich repeat-containing 52 (LRRC52). However, in human sperm it is controversial whether the pore forming subunit of the channel is composed of SLO3 and/or SLO1. Deciphering the role of the proton-specific Hv1 channel is more challenging as it is only expressed in human sperm. However, definitive evidence for a role in, and importance for, human fertility can only be determined through studies using clinical samples.OBJECTIVE AND RATIONALE: This review aims to provide insight into the role of sperm ion channels in human fertilization as evidenced from recent studies of sperm from infertile men. We also summarize the key discoveries from mouse ion channel knock-out models and contrast the properties of mouse and human CatSper and potassium currents. We detail the evidence for, and consequences of, defective ion channels in human sperm and discuss hypotheses to explain how defects arise and why affected sperm have impaired fertilization potential.SEARCH METHODS: Relevant studies were identified using PubMed and were limited to ion channels that have been characterized in mouse and human sperm. Additional notable examples from other species are included as appropriate.OUTCOMES: There are now well-documented fundamental differences between the properties of CatSper and potassium channel currents in mouse and human sperm. However, in both species, sperm lacking either channel cannot fertilize in vivo and CatSper-null sperm also fail to fertilize at IVF. Sperm-lacking potassium currents are capable of fertilizing at IVF, albeit at a much lower rate. However, additional complex and heterogeneous ion channel dysfunction has been reported in sperm from infertile men, the causes of which are unknown. Similarly, the nature of the functional impairment of affected patient sperm remains elusive. There are no reports of studies of Hv1 in human sperm from infertile men.WIDER IMPLICATIONS: Recent studies using sperm from infertile men have given new insight and critical evidence supporting the supposition that calcium and potassium conductances are essential for human fertility. However, it should be highlighted that many fundamental questions remain regarding the nature of molecular and functional defects in sperm with dysfunctional ion channels. The development and application of advanced technologies remains a necessity to progress basic and clinical research in this area, with the aim of providing effective screening methodologies to identify and develop treatments for affected men in order to help prevent failed ART cycles. Conversely, development of drugs that block calcium and/or potassium conductances in sperm is a plausible strategy for producing sperm-specific contraceptives.</p

O VI and Multicomponent H I Absorption Associated with a Galaxy Group in the Direction of PG0953+415: Physical Conditions and Baryonic Content

We report the discovery of an O VI absorption system at z(abs) = 0.14232 in a high resolution FUV spectrum of PG0953+415 obtained with the Space Telescope Imaging Spectrograph (STIS). Both lines of the O VI 1032, 1038 doublet and multicomponent H I Lya absorption are detected, but the N V doublet and the strong lines of C II and Si III are not apparent. We examine the ionization mechanism of the O VI absorber and find that while theoretical considerations favor collisional ionization, it is difficult to observationally rule out photoionization. If the absorber is collisionally ionized, it may not be in equilibrium due to the rapid cooling of gas in the appropriate temperature range. Non-equilibrium collisionally ionized models are shown to be consistent with the observations. A WIYN survey of galaxy redshifts near the sight line has revealed a galaxy at a projected distance of 395 kpc separated by ~130 km/s from this absorber, and three additional galaxies are found within 130 km/s of this redshift with projected separations ranging from 1.0 Mpc to 3.0 Mpc. Combining the STIS observations of PG0953+415 with previous high S/N GHRS observations of H1821+643, we derive a large number of O VI absorbers per unit redshift, dN/dz ~20. We use this sample to obtain a first estimate of the cosmological mass density of the O VI systems at z ~ 0. If further observations confirm the large dN/dz derived for the O VI systems, then these absorbers trace a significant reservoir of baryonic matter at low redshift.Comment: Accepted for publication in Ap.J., vol. 542 (Oct. 10, 2000