28,466 research outputs found
3D mesh processing using GAMer 2 to enable reaction-diffusion simulations in realistic cellular geometries
Recent advances in electron microscopy have enabled the imaging of single
cells in 3D at nanometer length scale resolutions. An uncharted frontier for in
silico biology is the ability to simulate cellular processes using these
observed geometries. Enabling such simulations requires watertight meshing of
electron micrograph images into 3D volume meshes, which can then form the basis
of computer simulations of such processes using numerical techniques such as
the Finite Element Method. In this paper, we describe the use of our recently
rewritten mesh processing software, GAMer 2, to bridge the gap between poorly
conditioned meshes generated from segmented micrographs and boundary marked
tetrahedral meshes which are compatible with simulation. We demonstrate the
application of a workflow using GAMer 2 to a series of electron micrographs of
neuronal dendrite morphology explored at three different length scales and show
that the resulting meshes are suitable for finite element simulations. This
work is an important step towards making physical simulations of biological
processes in realistic geometries routine. Innovations in algorithms to
reconstruct and simulate cellular length scale phenomena based on emerging
structural data will enable realistic physical models and advance discovery at
the interface of geometry and cellular processes. We posit that a new frontier
at the intersection of computational technologies and single cell biology is
now open.Comment: 39 pages, 14 figures. High resolution figures and supplemental movies
available upon reques
Dynamical system approach for edge detection using coupled FitzHugh–Nagumo neurons
The prospect of emulating the impressive computational capabilities of biological systems has led to considerable interest in the design of analog circuits that are potentially implementable in very large scale integration CMOS technology and are guided by biologically motivated models. For example, simple image processing tasks, such as the detection of edges in binary and grayscale images, have been performed by networks of FitzHugh-Nagumo-type neurons using the reaction-diffusion models. However, in these studies, the one-to-one mapping of image pixels to component neurons makes the size of the network a critical factor in any such implementation. In this paper, we develop a simplified version of the employed reaction-diffusion model in three steps. In the first step, we perform a detailed study to locate this threshold using continuous Lyapunov exponents from dynamical system theory. Furthermore, we render the diffusion in the system to be anisotropic, with the degree of anisotropy being set by the gradients of grayscale values in each image. The final step involves a simplification of the model that is achieved by eliminating the terms that couple the membrane potentials of adjacent neurons. We apply our technique to detect edges in data sets of artificially generated and real images, and we demonstrate that the performance is as good if not better than that of the previous methods without increasing the size of the network
Monitoring Galvanic Replacement Through Three-Dimensional Morphological and Chemical Mapping
Galvanic replacement reactions on metal nanoparticles are often used for the
preparation of hollow nanostructures with tunable porosity and chemical
composition, leading to tailored optical and catalytic properties. However, the
precise interplay between the three-dimensional (3D) morphology and chemical
composition of nanostructures during Galvanic replacement is not always well
understood as the 3D chemical imaging of nanoscale materials is still
challenging. It is especially far from straightforward to obtain detailed
information from the inside of hollow nanostructures using electron microscopy
techniques such as SEM or TEM. We demonstrate here that a combination of
state-of-the-art EDX mapping with electron tomography results in the
unambiguous determination of both morphology transformation and elemental
composition of nanostructures in 3D, during Galvanic replacement of Ag
nanocubes. This work provides direct and unambiguous experimental evidence
leading to new insights in the understanding of the galvanic replacement
reaction. In addition, the powerful approach presented here can be applied to a
wide range of nanoscale transformation processes, which will undoubtedly guide
the development of novel nanostructures
Image Segmentation with Eigenfunctions of an Anisotropic Diffusion Operator
We propose the eigenvalue problem of an anisotropic diffusion operator for
image segmentation. The diffusion matrix is defined based on the input image.
The eigenfunctions and the projection of the input image in some eigenspace
capture key features of the input image. An important property of the model is
that for many input images, the first few eigenfunctions are close to being
piecewise constant, which makes them useful as the basis for a variety of
applications such as image segmentation and edge detection. The eigenvalue
problem is shown to be related to the algebraic eigenvalue problems resulting
from several commonly used discrete spectral clustering models. The relation
provides a better understanding and helps developing more efficient numerical
implementation and rigorous numerical analysis for discrete spectral
segmentation methods. The new continuous model is also different from
energy-minimization methods such as geodesic active contour in that no initial
guess is required for in the current model. The multi-scale feature is a
natural consequence of the anisotropic diffusion operator so there is no need
to solve the eigenvalue problem at multiple levels. A numerical implementation
based on a finite element method with an anisotropic mesh adaptation strategy
is presented. It is shown that the numerical scheme gives much more accurate
results on eigenfunctions than uniform meshes. Several interesting features of
the model are examined in numerical examples and possible applications are
discussed
Contribution of X-ray CMT and image processing to the modelling of pyrocarbon Chemical Vapour Infiltration
International audienceThe Chemical Vapour Infiltration (CVI) process is used to fabricate the pyrocarbon matrices of C/C composites. This process involves complex physico-chemical phenomena such as the transport of precursor, carrier, and by-product gases in the reactor and inside a fibrous preform, heat transfer, chemical reactions (pyrolysis and deposition), and the structural evolution of the preform. It is able to provide high-quality materials because the processing conditions are rather mild with respect to the fibres; however it is expensive and sometimes difficult to optimize. This process has been the object of extensive modelling efforts, because of imperative optimization needs. The present work presents an approach suited to the exploitation of computerized microtomographs of C/C composites, which features image acquisition, computation of geometrical and transport properties, and infiltration modelling, as applied to the infiltration of needled carbon fibre fabrics. Another application to the reinforcement of carbon foams is also presented, as an example of inserting this approach in a global modelling strategy
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