A role for the extracellular calcium-sensing receptor in cell-cell communication in pancreatic islets of Langerhans

Background: The extracellular calcium-sensing receptor (CaR) is expressed in many tissues that are not associated with Ca2+ homeostasis, including the endocrine cells in pancreatic islets of Langerhans. We have demonstrated previously that pharmacological activation of the CaR stimulates insulin secretion from islet -cells and insulin-secreting MIN6 cells. Methods: In the present study we have investigated the effects of CaR activation on MIN6 cell proliferation and have used shRNA-mediated CaR knockdown to determine whether the CaR is involved in the regulation of insulin secretion via cell-cell communication. Results: CaR activation caused the phosphorylation and activation of the p42/44 MAPK signalling cascade, and this activation was prevented by the shRNA-induced down-regulation of CaR mRNA expression. CaR activation also resulted in increased proliferation of MIN6 cells, consistent with the known role of the p42/44 MAPK system in the regulation of -cell proliferation. Down-regulation of CaR expression had no detectable effects on glucose-induced insulin secretion from MIN6 cells maintained as monolayers, but blocked the increases in insulin secretion that were observed when the cells were configured as three-dimensional islet-like structures (pseudoislets), consistent with a role for the CaR in cell-cell communication in pseudoislets. Conclusion: It is well established that islet function is dependent on communication between islet cells and the results of this study suggest that the CaR is required for -cell to -cell interactions within islet-like structures

Simulation of Mixed Critical In-vehicular Networks

Future automotive applications ranging from advanced driver assistance to autonomous driving will largely increase demands on in-vehicular networks. Data flows of high bandwidth or low latency requirements, but in particular many additional communication relations will introduce a new level of complexity to the in-car communication system. It is expected that future communication backbones which interconnect sensors and actuators with ECU in cars will be built on Ethernet technologies. However, signalling from different application domains demands for network services of tailored attributes, including real-time transmission protocols as defined in the TSN Ethernet extensions. These QoS constraints will increase network complexity even further. Event-based simulation is a key technology to master the challenges of an in-car network design. This chapter introduces the domain-specific aspects and simulation models for in-vehicular networks and presents an overview of the car-centric network design process. Starting from a domain specific description language, we cover the corresponding simulation models with their workflows and apply our approach to a related case study for an in-car network of a premium car

Adaptive routing for intermittently connected mobile ad hoe networks

The vast majority of mobile ad hoc networking research makes a very large assumption: that communication can only take place between nodes that are simultaneously accessible within in the same connected cloud (i.e., that communication is synchronous). In reality, this assumption is likely to be a poor one, particularly for sparsely or irregularly populated environments.In this paper we present the Context-Aware Routing (CAR) algorithm. CAR is a novel approach to the provision of asynchronous communication in partially-connected mobile ad hoc networks, based on the intelligent placement of messages. We discuss the details of the algorithm, and then present simulation results demonstrating that it is possible for nodes to exploit context information in making local decisions that lead to good delivery ratios and latencies with small overheads.</p

Fault-Tolerance by Graceful Degradation for Car Platoons

The key advantage of autonomous car platoons are their short inter-vehicle distances that increase traffic flow and reduce fuel consumption. However, this is challenging for operational and functional safety. If a failure occurs, the affected vehicles cannot suddenly stop driving but instead should continue their operation with reduced performance until a safe state can be reached or, in the case of temporal failures, full functionality can be guaranteed again. To achieve this degradation, platoon members have to be able to compensate sensor and communication failures and have to adjust their inter-vehicle distances to ensure safety. In this work, we describe a systematic design of degradation cascades for sensor and communication failures in autonomous car platoons using the example of an autonomous model car. We describe our systematic design method, the resulting degradation modes, and formulate contracts for each degradation level. We model and test our resulting degradation controller in Simulink/Stateflow

IKLIM KOMUNIKASI ORGANISASI KOMUNITAS DJARUM BLACK CAR DI SURABAYA (Studi Deskriptif Iklim Komunikasi Komunitas Djarum Black Car di Surabaya

Good communication with an organization run smoothly and successfully, lack or absence of communication within organizations can lead to congestion or not working effectively. The way one builds a reaction to the organizational aspects of creating a climate of communication. The purpose of this study was to determine how the organization's communication climate in Djarum Black Car Community in Surabaya. To measure the Organizational Communication Climate variables: trust, shared decision-making, honesty, openness in communication down, listening in upward communication, attention to high-performance goals. This study population are members of Djarum Black car Surabaya numbering 50 people (Djarum Black Car Community) and the technique for determining the total sample using the method of sampling. Technique the analysis which used is the Climate Analysis of Communication. The results of this study is the value of 0.1158 Composite Climate. of these results shows the communication climate in the Djarum Black Car Commmunity in Surabaya are the coefficients whose value is less than 0.79, so that said Djarum Black Car Commmunity in Surabaya has a negative communication climate

Calcium-sensing receptor activation increases cell-cell adhesion and ß-cell function

Background/Aims: The extracellular calcium-sensing receptor (CaR) is expressed in pancreatic β-cells where it is thought to facilitate cell-to-cell communication and augment insulin secretion. However, it is unknown how CaR activation improves β-cell function. Methods: Immunocytochemistry and western blotting confirmed the expression of CaR in MIN6 β-cell line. The calcimimetic R568 (1µM) was used to increase the affinity of the CaR and specifically activate the receptor at a physiologically appropriate extracellular calcium concentration. Incorporation of 5-bromo-2’-deoxyuridine (BrdU) was used to measure cell proliferation, whilst changes in non-nutrient-evoked cytosolic calcium were assessed using fura-2-microfluorimetry. AFM-single-cell-force spectroscopy related CaR-evoked changes in epithelial (E)-cadherin expression to improved functional tethering between coupled cells. Results: Activation of the CaR over 48hr doubled the expression of E-cadherin (206±41%) and increased L-type voltage-dependent calcium channel expression by 70% compared to control. These changes produced a 30% increase in cell-cell tethering and elevated the basal-to-peak amplitude of ATP (50µM) and tolbutamide (100µM)-evoked changes in cytosolic calcium. Activation of the receptor also increased PD98059 (1-100µM) and SU1498 (1-100µM)-dependent β-cell proliferation. Conclusion: Our data suggest that activation of the CaR increases E-cadherin mediated functional tethering between β-cells and increases expression of L-type VDCC and secretagogue-evoked changes in [Ca2+]i. These findings could explain how local changes in calcium, co-released with insulin, activate the CaR on neighbouring cells to help ensure efficient and appropriate secretory function

Integration of Car-2-Car Communication as a Virtual Sensor in Automotive Sensor Fusion for Advanced Driver Assistance Systems

Advanced driver assistance systems (ADAS) require a comprehensive and accurate situation model. Often in-vehicle sensors do not provide sufficient quality and quantity of information to fulfill the demanding requirements. Car-2-Car communication can be seen as an adaptive sensor that provides additional information regularly but also on demand. Due to the fact that Car-2-Car communication strongly depends on the penetration rate, we argue for a seamless integration of Car-2-Car communication as an additional sensor in automotive sensor fusion. With increasing penetration rate the sensor fusion will significantly benefit and eventually unfold its full potential. Due to the fundamentally different measuring principles of in-vehicle sensors and information provided by Car-2-Car communication, redundancy and complementarity can be leveraged to a great extent, thus, increasing accuracy, reliability and robustness of the situation assessment. In addition to a detailed description of the fusion algorithm this paper outlines DLR’s system architecture for ADAS and an enhanced ACC as an application example to show the potential of our approach