366 research outputs found
Shape mode analysis exposes movement patterns in biology: flagella and flatworms as case studies
We illustrate shape mode analysis as a simple, yet powerful technique to
concisely describe complex biological shapes and their dynamics. We
characterize undulatory bending waves of beating flagella and reconstruct a
limit cycle of flagellar oscillations, paying particular attention to the
periodicity of angular data. As a second example, we analyze non-convex
boundary outlines of gliding flatworms, which allows us to expose stereotypic
body postures that can be related to two different locomotion mechanisms.
Further, shape mode analysis based on principal component analysis allows to
discriminate different flatworm species, despite large motion-associated shape
variability. Thus, complex shape dynamics is characterized by a small number of
shape scores that change in time. We present this method using descriptive
examples, explaining abstract mathematics in a graphic way.Comment: 20 pages, 6 figures, accepted for publication in PLoS On
Ciliary contact interactions dominate surface scattering of swimming eukaryotes
Interactions between swimming cells and surfaces are essential to many
microbiological processes, from bacterial biofilm formation to human
fertilization. However, in spite of their fundamental importance, relatively
little is known about the physical mechanisms that govern the scattering of
flagellated or ciliated cells from solid surfaces. A more detailed
understanding of these interactions promises not only new biological insights
into structure and dynamics of flagella and cilia, but may also lead to new
microfluidic techniques for controlling cell motility and microbial locomotion,
with potential applications ranging from diagnostic tools to therapeutic
protein synthesis and photosynthetic biofuel production. Due to fundamental
differences in physiology and swimming strategies, it is an open question
whether microfluidic transport and rectification schemes that have recently
been demonstrated for pusher-type microswimmers such as bacteria and sperm
cells, can be transferred to puller-type algae and other motile eukaryotes, as
it is not known whether long-range hydrodynamic or short-range mechanical
forces dominate the surface interactions of these microorganisms. Here, using
high-speed microscopic imaging, we present direct experimental evidence that
the surface scattering of both mammalian sperm cells and unicellular green
algae is primarily governed by direct ciliary contact interactions. Building on
this insight, we predict and verify experimentally the existence of optimal
microfluidic ratchets that maximize rectification of initially uniform
Chlamydomonas reinhardtii suspensions. Since mechano-elastic properties of
cilia are conserved across eukaryotic species, we expect that our results apply
to a wide range of swimming microorganisms.Comment: Preprint as accepted for publication in PNAS, for published journal
version (open access) and Supporting Information see
http://dx.doi.org/10.1073/pnas.121054811
Swimming by spinning: spinning-top type rotations regularize sperm swimming into persistently symmetric paths in 3D
Sperm modulate their flagellar symmetry to navigate through complex
physico-chemical environments and achieve reproductive function. Yet it remains
elusive how sperm swim forwards despite the inherent asymmetry of several
components that constitutes the flagellar engine. Despite the critical
importance of symmetry, or the lack of it, on sperm navigation and its
physiological state, there is no methodology to date that can robustly detect
the symmetry state of the beat in free-swimming sperm in 3D.How does symmetric
progressive swimming emerge even for asymmetric beating, and how can beating
(a)symmetry be inferred experimentally? Here, we numerically resolve the fluid
mechanics of swimming around asymmetrically beating spermatozoa. This reveals
that sperm spinning critically regularizes swimming into persistently symmetric
paths in 3D, allowing sperm to swim forwards despite any imperfections on the
beat. The sperm orientation in three-dimensions, and not the swimming path, can
inform the symmetry state of the beat, eliminating the need of tracking the
flagellum in 3D. We report a surprising correspondence between the movement of
sperm and spinning-top experiments, indicating that the flagellum drives
''spinning-top'' type rotations during sperm swimming, and that this parallel
is not a mere analogy. These results may prove essential in future studies on
the role of (a)symmetry in spinning and swimming microorganisms and
micro-robots, as body orientation detection has been vastly overlooked in
favour of swimming path detection. Altogether, sperm rotation may provide a
foolproof mechanism for forward propulsion and navigation in nature that would
otherwise not be possible for flagella with broken symmetry
A dynamic basal complex modulates mammalian sperm movement
Centrioles are ancient organelles with a conserved architecture and their rigidity is thought to restrict microtubule sliding. Here authors show that, in mammalian sperm, the atypical distal centriole and its surrounding atypical pericentriolar matrix form a dynamic basal complex that facilitates a cascade of internal sliding deformations, coupling tail beating with asymmetric head kinking
Unlocking the Secrets of Multi-Flagellated Propulsion
In this work, unique high-speed imaging platforms and an array of theoretical analysis methods are used to thoroughly investigate eukaryotic multi-flagellated propulsion using Tritrichomonas foetus as a test case. Through experimental observations through our imaging system with superior resolution and capture rate exceeding that of previous studies, it was discovered for the first time that the T. foetus employs a strategy similar to that of the “run and tumble” strategies found in bacteria and Chlamydomonas; it has two distinct flagellar beating patterns that result in two different body swimming motions, linear and turning swimming.
These two flagella patterns were then analyzed for the first time using two theoretical analysis methods that are often used to analyze uni-flagellated organisms; the Resistive Force Theory (RFT) and the Regularized Stokeslet Method (RSM). These theories were compared to uncover the more accurate method. Results showed that our modified-RFT model out-performed the RSM model. Due to these results, the quantitative analysis of the motion of each flagellum for both the swimming motions were carried out using the RFT method for the first time on a multi-flagellated cell, in both the 2-D and 3-D case.
Digital Holographic Microscopy was used to produce the 3-D trajectory of the T.foetus for the first time. Through this method it was possible to for the first time, quantitatively analyze the thrust and energy contributions of each flagella in each direction. We find out that the turning motion dissipates approximately half as much energy as the linear swimming motion which leads to the belief that the motion is more energy efficient. The energy results coupled with the thrust results show the highly coordinated nature of multi-flagellated propulsion. Through this RFT model, it was observed that the propulsive force of the T.foetus is comparable to that of other eukaryotes with varying numbers of flagella like the sperm and Chlamydomonas, suggesting that higher thrust generation is not necessarily the goal of multi-flagellated propulsion, but these strategies result in greater maneuverability or sensing. Results from this study may serve as inspiration for biorobots due to the organism’s ideal size and finely controlled multi-flagellated propulsion
Physics of Microswimmers - Single Particle Motion and Collective Behavior
Locomotion and transport of microorganisms in fluids is an essential aspect
of life. Search for food, orientation toward light, spreading of off-spring,
and the formation of colonies are only possible due to locomotion. Swimming at
the microscale occurs at low Reynolds numbers, where fluid friction and
viscosity dominates over inertia. Here, evolution achieved propulsion
mechanisms, which overcome and even exploit drag. Prominent propulsion
mechanisms are rotating helical flagella, exploited by many bacteria, and
snake-like or whip-like motion of eukaryotic flagella, utilized by sperm and
algae. For artificial microswimmers, alternative concepts to convert chemical
energy or heat into directed motion can be employed, which are potentially more
efficient. The dynamics of microswimmers comprises many facets, which are all
required to achieve locomotion. In this article, we review the physics of
locomotion of biological and synthetic microswimmers, and the collective
behavior of their assemblies. Starting from individual microswimmers, we
describe the various propulsion mechanism of biological and synthetic systems
and address the hydrodynamic aspects of swimming. This comprises
synchronization and the concerted beating of flagella and cilia. In addition,
the swimming behavior next to surfaces is examined. Finally, collective and
cooperate phenomena of various types of isotropic and anisotropic swimmers with
and without hydrodynamic interactions are discussed.Comment: 54 pages, 59 figures, review article, Reports of Progress in Physics
(to appear
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