A graph-based mathematical morphology reader

This survey paper aims at providing a "literary" anthology of mathematical morphology on graphs. It describes in the English language many ideas stemming from a large number of different papers, hence providing a unified view of an active and diverse field of research

Analyses of the Watershed Transform

International audienceIn the framework of mathematical morphology, watershed transform (WT) represents a key step in image segmentation procedure. In this paper, we present a thorough analysis of some existing watershed approaches in the discrete case: WT based on flooding, WT based on path-cost minimization, watershed based on topology preservation, WT based on local condition and WT based on minimum spanning forest. For each approach, we present detailed description of processing procedure followed by mathematical foundations and algorithm of reference. Recent publications based on some approaches are also presented and discussed. Our study concludes with a classification of different watershed transform algorithms according to solution uniqueness, topology preservation, prerequisites minima computing and linearity

Tie-zone : the bridge between watershed transforms and fuzzy connectedness

Orientador: Roberto de Alencar LotufoTese (doutorado) - Universidade Estadual de Campinas, Faculdade de Engenharia Eletrica e de ComputaçãoResumo: Esta tese introduz o novo conceito de transformada de zona de empate que unifica as múltiplas soluções de uma transformada de watershed, conservando apenas as partes comuns em todas estas, tal que as partes que diferem constituem a zona de empate. A zona de empate aplicada ao watershed via transformada imagem-floresta (TZ-IFT-WT) se revela um elo inédito entre transformadas de watershed baseadas em paradigmas muito diferentes: gota d'água, inundação, caminhos ótimos e floresta de peso mínimo. Para todos esses paradigmas e os algoritmos derivados, é um desafio se ter uma solução única, fina, e que seja consistente com uma definição. Por isso, propõe-se um afinamento da zona de empate, único e consistente. Além disso, demonstra-se que a TZ-IFT-WT também é o dual de métodos de segmentação baseados em conexidade nebulosa. Assim, a ponte criada entre as abordagens morfológica e nebulosa permite aproveitar avanços de ambas. Em conseqüência disso, o conceito de núcleo de robustez para as sementes é explorado no caso do watershed.Abstract: This thesis introduces the new concept of tie-zone transform that unifies the multiple solutions of a watershed transform, by conserving only the common parts among them such that the differing parts constitute the tie zone. The tie zone applied to the watershed via image-foresting transform (TZ-IFTWT) proves to be a link between watershed transforms based on very different paradigms: drop of water, flooding, optimal paths and forest of minimum weight. For all these paradigms and the derived algorithms, it is a challenge to get a unique and thin solution which is consistent with a definition. That is why we propose a unique and consistent thinning of the tie zone. In addition, we demonstrate that the TZ-IFT-WT is also the dual of segmentation methods based on fuzzy connectedness. Thus, the bridge between the morphological and the fuzzy approaches allows to take benefit from the advance of both. As a consequence, the concept of cores of robustness for the seeds is exploited in the case of watersheds.DoutoradoEngenharia de ComputaçãoDoutor em Engenharia Elétric

Uma abordagem de agrupamento baseada na técnica de divisão e conquista e floresta de caminhos ótimos

Orientador: Alexandre Xavier FalcãoDissertação (mestrado) - Universidade Estadual de Campinas, Instituto de ComputaçãoResumo: O agrupamento de dados é um dos principais desafios em problemas de Ciência de Dados. Apesar do seu progresso científico em quase um século de existência, algoritmos de agrupamento ainda falham na identificação de grupos (clusters) naturalmente relacionados com a semântica do problema. Ademais, os avanços das tecnologias de aquisição, comunicação, e armazenamento de dados acrescentam desafios cruciais com o aumento considerável de dados, os quais não são tratados pela maioria das técnicas. Essas questões são endereçadas neste trabalho através da proposta de uma abordagem de divisão e conquista para uma técnica de agrupamento única em encontrar um grupo por domo da função de densidade de probabilidade dos dados --- o algoritmo de agrupamento por floresta de caminhos ótimos (OPF - Optimum-Path Forest). Nesta técnica, amostras são interpretadas como nós de um grafo cujos arcos conectam os $k$-vizinhos mais próximos no espaço de características. Os nós são ponderados pela sua densidade de probabilidade e um mapa de conexidade é maximizado de modo que cada máximo da função densidade de probabilidade se torna a raiz de uma árvore de caminhos ótimos (grupo). O melhor valor de $k$ é estimado por otimização em um intervalo de valores dependente da aplicação. O problema com este método é que um número alto de amostras torna o algoritmo inviável, devido ao espaço de memória necessário para armazenar o grafo e o tempo computacional para encontrar o melhor valor de $k$. Visto que as soluções existentes levam a resultados ineficazes, este trabalho revisita o problema através da proposta de uma abordagem de divisão e conquista com dois níveis. No primeiro nível, o conjunto de dados é dividido em subconjuntos (blocos) menores e as amostras pertencentes a cada bloco são agrupadas pelo algoritmo OPF. Em seguida, as amostras representativas de cada grupo (mais especificamente as raízes da floresta de caminhos ótimos) são levadas ao segundo nível, onde elas são agrupadas novamente. Finalmente, os rótulos de grupo obtidos no segundo nível são transferidos para todas as amostras do conjunto de dados através de seus representantes do primeiro nível. Nesta abordagem, todas as amostras, ou pelo menos muitas delas, podem ser usadas no processo de aprendizado não supervisionado, sem afetar a eficácia do agrupamento e, portanto, o procedimento é menos susceptível a perda de informação relevante ao agrupamento. Os resultados mostram agrupamentos satisfatórios em dois cenários, segmentação de imagem e agrupamento de dados arbitrários, tendo como base a comparação com abordagens populares. No primeiro cenário, a abordagem proposta atinge os melhores resultados em todas as bases de imagem testadas. No segundo cenário, os resultados são similares aos obtidos por uma versão otimizada do método original de agrupamento por floresta de caminhos ótimosAbstract: Data clustering is one of the main challenges when solving Data Science problems. Despite its progress over almost one century of research, clustering algorithms still fail in identifying groups naturally related to the semantics of the problem. Moreover, the advances in data acquisition, communication, and storage technologies add crucial challenges with a considerable data increase, which are not handled by most techniques. We address these issues by proposing a divide-and-conquer approach to a clustering technique, which is unique in finding one group per dome of the probability density function of the data --- the Optimum-Path Forest (OPF) clustering algorithm. In the OPF-clustering technique, samples are taken as nodes of a graph whose arcs connect the $k$-nearest neighbors in the feature space. The nodes are weighted by their probability density values and a connectivity map is maximized such that each maximum of the probability density function becomes the root of an optimum-path tree (cluster). The best value of $k$ is estimated by optimization within an application-specific interval of values. The problem with this method is that a high number of samples makes the algorithm prohibitive, due to the required memory space to store the graph and the computational time to obtain the clusters for the best value of $k$. Since the existing solutions lead to ineffective results, we decided to revisit the problem by proposing a two-level divide-and-conquer approach. At the first level, the dataset is divided into smaller subsets (blocks) and the samples belonging to each block are grouped by the OPF algorithm. Then, the representative samples (more specifically the roots of the optimum-path forest) are taken to a second level where they are clustered again. Finally, the group labels obtained in the second level are transferred to all samples of the dataset through their representatives of the first level. With this approach, we can use all samples, or at least many samples, in the unsupervised learning process without affecting the grouping performance and, therefore, the procedure is less likely to lose relevant grouping information. We show that our proposal can obtain satisfactory results in two scenarios, image segmentation and the general data clustering problem, in comparison with some popular baselines. In the first scenario, our technique achieves better results than the others in all tested image databases. In the second scenario, it obtains outcomes similar to an optimized version of the traditional OPF-clustering algorithmMestradoCiência da ComputaçãoMestre em Ciência da ComputaçãoCAPE

DeadEasy Mito-Glia: Automatic Counting of Mitotic Cells and Glial Cells in Drosophila

Cell number changes during normal development, and in disease (e.g., neurodegeneration, cancer). Many genes affect cell number, thus functional genetic analysis frequently requires analysis of cell number alterations upon loss of function mutations or in gain of function experiments. Drosophila is a most powerful model organism to investigate the function of genes involved in development or disease in vivo. Image processing and pattern recognition techniques can be used to extract information from microscopy images to quantify automatically distinct cellular features, but these methods are still not very extended in this model organism. Thus cellular quantification is often carried out manually, which is laborious, tedious, error prone or humanly unfeasible. Here, we present DeadEasy Mito-Glia, an image processing method to count automatically the number of mitotic cells labelled with anti-phospho-histone H3 and of glial cells labelled with anti-Repo in Drosophila embryos. This programme belongs to the DeadEasy suite of which we have previously developed versions to count apoptotic cells and neuronal nuclei. Having separate programmes is paramount for accuracy. DeadEasy Mito-Glia is very easy to use, fast, objective and very accurate when counting dividing cells and glial cells labelled with a nuclear marker. Although this method has been validated for Drosophila embryos, we provide an interactive window for biologists to easily extend its application to other nuclear markers and other sample types. DeadEasy MitoGlia is freely available as an ImageJ plug-in, it increases the repertoire of tools for in vivo genetic analysis, and it will be of interest to a broad community of developmental, cancer and neuro-biologists

Geochemical interactions in geological hydrogen Storage: The role of sandstone clay content

Hydrogen holds promise as a clean energy alternative, crucial for achieving global decarbonization goals and net-zero carbon emissions. Its low volumetric energy density necessitates underground storage in sandstone formations to maintain year-round supply. The efficacy of such storage hinges on the geochemical interplay between hydrogen and the host sandstone. Despite the slow reaction rates in sandstone, the influence of its clay composition on hydrogen interaction remains underexplored. In this study, we specifically investigate the geochemical interactions of hydrogen with clay-bearing sandstone formations under controlled conditions, simulating storage scenarios. This study evaluates the impact of clay on hydrogen-sandstone geochemistry after 75 days of injection at 1500 psi and 75 °C into Berea and Bandera gray sandstone cores, utilizing microcomputed tomography to assess changes in pore structure. Our results reveal that, even in sandstones with high clay content, there is negligible alteration in porosity and mineral content, as well as minimal clay and quartz dissolution or expansion over storage time, indicating stability in these formations. These findings provide crucial insights for selecting suitable geological formations for hydrogen storage, supporting the global shift towards sustainable energy systems Our study contributes to the global efforts in decarbonization by providing essential guidance on the feasibility of using clay-bearing sandstone formations for efficient and sustainable hydrogen storage