Herstellung eines Phaffia rhodozyma : Stamms mit verstärkter Astaxanthin-Synthese über gezielte genetische Modifikation chemisch mutagenisierter Stämme

Ziel dieser Arbeit war es erstmals durch eine Kombination aus chemischer Mutagenese und gezielter genetischer Modifikation (hier: „metabolic engineering“) einen Phaffia-Stamm herzustellen, welcher über die Mutagenese hinaus über eine weiter verstärkte Astaxanthin-Synthese verfügt. Die von „DSM Nutritional Products“ bereitgestellten chemischen Mutanten wurden analysiert und über einen Selektionsprozess auf Pigmentstabilität und Wachstum hin optimiert, da die Stämme aus cryogenisierter Dauerkultur starke Pigmentinstabilitäten und ein verzögertes Wachstum aufwiesen. Über eine exploratorische Phase wurde die Carotinoidsynthese analysiert und festgestellt, dass in den Mutanten keine Einzelreaktionen betroffen sind, welche für die Heraufregulierung der Carotinoidsynthese in den Mutanten verantwortlich sind. Hierbei wurden Limitierungen identifiziert und diese durch Transformation von Expressionsplasmiden mit geeigneten Genen aufgehoben, um damit eine noch effizientere Metabolisierung von Astaxanthin-Vorstufen hin zu Astaxanthin zu erreichen. Eine Überexpression der Phytoensynthase/Lycopinzyklase crtYB resultierte in einem gesteigerten Carotinoidgehalt bei gleichbleibendem Astaxanthin- Anteil. Durch eine zweite Transformation mit einer Expressionskassette für die Astaxanthin-Synthase asy konnte der Carotinoidgehalt weiter gesteigert und zusätzlich eine Limitierung der Metabolisierung von Astaxanthin-Vorstufen behoben werden, sodass die Transformante nahezu alle Intermediate der Astaxanthinsynthese zu Astaxanthin metabolisieren konnte (Gassel et al. 2013). Es konnte gezeigt werden, dass auch in den Mutanten, aus Experimenten mit dem Wildtyp bekannte, Limitierungen identifiziert und ausgeglichen werden konnten

Spectacular narratives: Twister, independence day, and frontier mythology

Big-screen spectacle has become increasingly important to Hollywood in recent decades. It formed a central part of a post-war strategy aimed at tempting lost audiences back to the cinema in the face of demographic changes and the development of television and other domestic leisure activities. More recently, in an age in which the big Hollywood studios have become parts of giant conglomerates, the prevalence of spectacle and special effects has been boosted by a demand to engineer products that can be further exploited in multimedia forms such as computer games and theme-park rides, secondary outlets that can sometimes generate more profits than the films on which they are based. These and other developments have led some commentators to announce, or predict, the imminent demise of narrative as a central component of Hollywood cinema. But the case has been considerably overstated. Narrative is far from being eclipsed, even in the most spectacular and effects-oriented of today’s blockbuster attractions. These films still tend to tell reasonably coherent stories, even if they may sometimes be looser and less well integrated than classical models. More important for my argument, contemporary spectaculars also continue to manifest the kinds of underlying thematic oppositions and reconciliations associated with a broadly ‘structuralist’ analysis of narrative. This very important dimension of narrative has been largely ignored by those who identify, celebrate or more often bemoan a weakening of plot or character development in many spectacular features

Towards a new theory of practice for community health psychology

The article sets out the value of theorizing collective action from a social science perspective that engages with the messy actuality of practice. It argues that community health psychology relies on an abstract version of Paulo Freire’s earlier writing, the Pedagogy of the Oppressed, which provides scholar-activists with a ‘map’ approach to collective action. The article revisits Freire’s later work, the Pedagogy of Hope, and argues for the importance of developing a ‘journey’ approach to collective action. Theories of practice are discussed for their value in theorizing such journeys, and in bringing maps (intentions) and journeys (actuality) closer together

A Phoenician Way to be Roman

This paper offers a synthesis on my dissertation, titled The Phoenician communities of the Iberian Peninsula and their integration in the Roman world: an identity perspective. The period under discussion extends from the end of the Second Punic War in 206 BCE to the Flavian era in the last quarter of the century CE. About all, this work focuses on the cultural and ethnic dimensions of the process of integration.Universidad de Málaga. Campus de Excelencia Internacional Andalucía Tech

A formal concept view of argumentation

International audienceThe paper presents a parallel between two important theories for the treatment of information which address questions that are apparently unrelated and that are studied by different research communities: an enriched view of formal concept analysis and abstract argumentation. Both theories exploit a binary relation (expressing object-property links, attacks between arguments). We show that when an argumentation framework rather considers the complementary relation does not attack, then its stable extensions can be seen as the exact counterparts of formal concepts. This leads to a cube of oppositions, a generalization of the well-known square of oppositions, between eight remarkable sets of arguments. This provides a richer view for argumentation in cases of bi-valued attack relations and fuzzy ones

First verbs : On the way to mini-paradigms

This 18th issue of ZAS-Papers in Linguistics consists of papers on the development of verb acquisition in 9 languages from the very early stages up to the onset of paradigm construction. Each of the 10 papers deals with first-Ianguage developmental processes in one or two children studied via longitudinal data. The languages involved are French, Spanish, Russian, Croatian, Lithuanien, Finnish, English and German. For German two different varieties are examined, one from Berlin and one from Vienna. All papers are based on presentations at the workshop 'Early verbs: On the way to mini-paradigms' held at the ZAS (Berlin) on the 30./31. of September 2000. This workshop brought to a close the first phase of cooperation between two projects on language acquisition which has started in October 1999: a) the project on "Syntaktische Konsequenzen des Morphologieerwerbs" at the ZAS (Berlin) headed by Juergen Weissenborn and Ewald Lang, and financially supported by the Deutsche Forschungsgemeinschaft, and b) the international "Crosslinguistic Project on Pre- and Protomorphology in Language Acquisition" coordinated by Wolfgang U. Dressler in behalf of the Austrian Academy of Sciences

The Daredevils of Sassoun: The Deep Structure of the Plot

The plot can be considered as the content, as well as the pattern of semantic organization of the text. In this regard we suggest making a distinction between deep and surface levels of the plot. In respect to the Armenian epic The Daredevils of Sassoun this distinction provides real opportunity to reveal the integrity and coherence of the epic viewed as a unified set of all of its various branches, versions, episodes and even variants, – despite that, on the surface level, the cohesion between and within the various branches of the epic is rather weak. The underlying semantic structure is based on two associated axes (patrilineage – matrilineage; patrilocality – matrilocality) and two fundamental oppositions (masculine – feminine; own – alien). The deep plot of the epic can be understood as a representation of the transition from the matrilineal (matrilocal) system in its radical form (denying men’s role in childbirth) to the opposing radical patrilineal system denying women’s role in its absolute and, therefore, tragic form (denying continuation of life). The well-known Lévi-Straussian quasi-algebraic formula of the semantics of myth can be used as an instrument for examining a formal representation of this plot

Reductio ad discrimen: Where features come from

This paper addresses two fundamental questions about the nature of formal features in phonology and morphosyntax: what is their expressive power, and where do they come from? To answer these questions, we begin with the most restrictive possible hypothesis (all features are privative, and are wholly dictated by Universal Grammar, with no room for cross-linguistic variation), and examine the extent to which empirical evidence from a variety of languages compels a retreat from this position. We argue that there is little to be gained by positing a universal set of specific features, and propose instead that the crucial contribution of UG is the language learner's ability to construct features by identifying correlations between contrasts at different levels of linguistic structure. This view resonates with current research on how the interaction between UG and external 'third factors' shapes the structure of language, while at the same time harking back to the Saussurean notion that contrast is the central function of linguistic representations