21,017 research outputs found
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The role of HG in the analysis of temporal iteration and interaural correlation
Functional Organization of Visual Cortex in the Owl Monkey
In this study, we compared the organization of orientation preference in visual areas V1, V2, and V3. Within these visual areas, we also
quantified the relationship between orientation preference and cytochrome oxidase (CO) staining patterns. V1 maps of orientation
preference contained both pinwheels and linear zones. The location of CO blobs did not relate in a systematic way to maps of orientation;
although, as in other primates, there were approximately twice as many pinwheels as CO blobs. V2 contained bands of high and low
orientation selectivity. The bands of high orientation selectivity were organized into pinwheels and linear zones, but iso-orientation
domains were twice as large as those in V1. Quantitative comparisons between bands containing high or low orientation selectivity and
CO dark and light bands suggested that at least four functional compartments exist in V2, CO dense bands with either high or low
orientation selectivity, and CO light bands with either high or low selectivity. We also demonstrated that two functional compartments
exist in V3, with zones of high orientation selectivity corresponding to CO dense areas and zones of low orientation selectivity corresponding
to CO pale areas. Together with previous findings, these results suggest that the modular organization of V1 is similar across
primates and indeed across most mammals. V2 organization in owl monkeys also appears similar to that of other simians but different
from that of prosimians and other mammals. Finally, V3 of owl monkeys shows a compartmental organization for orientation selectivity
that remains to be demonstrated in other primates
Evaluation of rigid registration methods for whole head imaging in diffuse optical tomography
Functional brain imaging has become an important neuroimaging technique for the study of brain organization and development. Compared to other imaging techniques, diffuse optical tomography (DOT) is a portable and low-cost technique that can be applied to infants and hospitalized patients using an atlas-based light model. For DOT imaging, the accuracy of the forward model has a direct effect on the resulting recovered brain function within a field of view and so the accuracy of the spatially normalized atlas-based forward models must be evaluated. Herein, the accuracy of atlas-based DOT is evaluated on models that are spatially normalized via a number of different rigid registration methods on 24 subjects. A multileveled approach is developed to evaluate the correlation of the geometrical and sensitivity accuracies across the full field of view as well as within specific functional subregions. Results demonstrate that different registration methods are optimal for recovery of different sets of functional brain regions. However, the “nearest point to point” registration method, based on the EEG 19 landmark system, is shown to be the most appropriate registration method for image quality throughout the field of view of the high-density cap that covers the whole of the optically accessible cortex
Visualization-Based Mapping of Language Function in the Brain
Cortical language maps, obtained through intraoperative electrical stimulation studies, provide a rich source of information for research on language organization. Previous studies have shown interesting correlations between the distribution of essential language sites and such behavioral indicators as verbal IQ and have provided suggestive evidence for regarding human language cortex as an organization of multiple distributed systems. Noninvasive studies using ECoG, PET, and functional MR lend support to this model; however, there as yet are no studies that integrate these two forms of information. In this paper we describe a method for mapping the stimulation data onto a 3-D MRI-based neuroanatomic model of the individual patient. The mapping is done by comparing an intraoperative photograph of the exposed cortical surface with a computer-based MR visualization of the surface, interactively indicating corresponding stimulation sites, and recording 3-D MR machine coordinates of the indicated sites. Repeatability studies were performed to validate the accuracy of the mapping technique. Six observers—a neurosurgeon, a radiologist, and four computer scientists, independently mapped 218 stimulation sites from 12 patients. The mean distance of a mapping from the mean location of each site was 2.07 mm, with a standard deviation of 1.5 mm, or within 5.07 mm with 95% confidence. Since the surgical sites are accurate within approximately 1 cm, these results show that the visualization-based approach is accurate within the limits of the stimulation maps. When incorporated within the kind of information system envisioned by the Human Brain Project, this anatomically based method will not only provide a key link between noninvasive and invasive approaches to understanding language organization, but will also provide the basis for studying the relationship between language function and anatomical variability
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Simultaneous mesoscopic and two-photon imaging of neuronal activity in cortical circuits.
Spontaneous and sensory-evoked activity propagates across varying spatial scales in the mammalian cortex, but technical challenges have limited conceptual links between the function of local neuronal circuits and brain-wide network dynamics. We present a method for simultaneous cellular-resolution two-photon calcium imaging of a local microcircuit and mesoscopic widefield calcium imaging of the entire cortical mantle in awake mice. Our multi-scale approach involves a microscope with an orthogonal axis design where the mesoscopic objective is oriented above the brain and the two-photon objective is oriented horizontally, with imaging performed through a microprism. We also introduce a viral transduction method for robust and widespread gene delivery in the mouse brain. These approaches allow us to identify the behavioral state-dependent functional connectivity of pyramidal neurons and vasoactive intestinal peptide-expressing interneurons with long-range cortical networks. Our imaging system provides a powerful strategy for investigating cortical architecture across a wide range of spatial scales
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