Acoustic power distribution techniques for wireless sensor networks

Recent advancements in wireless power transfer technologies can solve several residual problems concerning the maintenance of wireless sensor networks. Among these, air-based acoustic systems are still less exploited, with considerable potential for powering sensor nodes. This thesis aims to understand the significant parameters for acoustic power transfer in air, comprehend the losses, and quantify the limitations in terms of distance, alignment, frequency, and power transfer efficiency. This research outlines the basic concepts and equations overlooking sound wave propagation, system losses, and safety regulations to understand the prospects and limitations of acoustic power transfer. First, a theoretical model was established to define the diffraction and attenuation losses in the system. Different off-the-shelf transducers were experimentally investigated, showing that the FUS-40E transducer is most appropriate for this work. Subsequently, different load-matching techniques are analysed to identify the optimum method to deliver power. The analytical results were experimentally validated, and complex impedance matching increased the bandwidth from 1.5 to 4 and the power transfer efficiency from 0.02% to 0.43%. Subsequently, a detailed 3D profiling of the acoustic system in the far-field region was provided, analysing the receiver sensitivity to disturbances in separation distance, receiver orientation and alignment. The measured effects of misalignment between the transducers are provided as a design graph, correlating the output power as a function of separation distance, offset, loading methods and operating frequency. Finally, a two-stage wireless power network is designed, where energy packets are inductively delivered to a cluster of nodes by a recharge vehicle and later acoustically distributed to devices within the cluster. A novel dynamic recharge scheduling algorithm that combines weighted genetic clustering with nearest neighbour search is developed to jointly minimise vehicle travel distance and power transfer losses. The efficacy and performance of the algorithm are evaluated in simulation using experimentally derived traces that presented 90% throughput for large, dense networks.Open Acces

Design of a Small, Affordable Low Intensity Focused Ultrasound Device for Vagus Nerve Stimulation

Depression is a serious public health issue that affects more than 300 million people worldwide. While there are antidepressant drugs to alleviate depressive symptoms, 10 – 30% of patients either do not respond or develop a tolerance to these drugs. Researchers have found a correlation between the inflammatory response and treatment-resistant depression (TRD). Blocking this inflammatory pathway with electrical vagus nerve stimulation (VNS) can reduce cytokine levels and depressive symptoms. However, placing an electrical VNS device is invasive, costly, and poses a risk to the vagus nerve. Low intensity focused ultrasound (LIFU) is a novel therapy that is able to both excite and suppress neuronal activity in neurological disorders. However, progression of this research area has been impeded by the size and price of these devices. I designed a 50 x 57 x 76 mm LIFU device that consists of a transducer, matching network, and amplification network. Next, I characterized my LIFU device with 2D intensity maps of the focused ultrasound (FUS) field. My device produced an instantaneous intensity up to 350 mW/cm2. My colleagues and I applied the LIFU device on Sprague-Dawley rats (n=12) for VNS with the primary goal of reducing the inflammatory response. Five out of the eight rats that we analyzed showed a decrease in the cytokine TNF-α. Future work will involve design improvements and more animal studies with varying stimulation parameters. As FUS technology becomes smaller we move closer to wearable devices. As FUS technology becomes more affordable more research groups will have the opportunity to employ this novel therapy to investigate the pathophysiology of neurological disorders

Intravascular Detection of Microvessel Infiltration in Atherosclerotic Plaques: An Intraluminal Extension of Acoustic Angiography

Cardiovascular disease is the leading cause of death worldwide, surpassing both stroke and cancer related mortality with 17.5 million deaths in 2014 alone. Atherosclerosis is the build-up of fatty deposits within arteries and is responsible for the majority of cardiovascular related deaths. Over the past decade, research in atherosclerosis has identified that a key limitation in the appropriate management of the disease is detecting and identifying dangerous fatty plaque build-ups before they dislodge and cause major cardiovascular events, such as embolisms, stroke, or myocardial infarctions. It has been noted that plaques vulnerable to rupture have several key features that may be used to distinguish them from asymptomatic plaques. One key identifier of a dangerous plaque is the presence of blood flow within the plaque itself since this is an indicator of growth and instability of the plaque. Recently, a superharmonic imaging method known as “acoustic angiography” has been shown to resolve microvasculature with unprecedented quality and could be a possible method of detecting blood vessel infiltration within these plaques. This dissertation describes the material and methods used to move the application of “acoustic angiography” to a reduced form factor typical of intravascular catheters and to demonstrate its ability to detect microvasculature. The implementation of this approach is described in terms of the contrast agents used to generate superharmonic signals, the dual-frequency transducers to image them, and the hardware needed to operate them in order to establish how these design choices can impact the quality of the images produced. Furthermore, this dissertation demonstrates how image processing methods such as adaptive windowing or automated sound speed correction can further enhance image quality of vascular targets. The results of these chapters show how acoustic angiography may be optimized using engineering considerations both in signal acquisition and post processing. Overall, these studies demonstrate that acoustic angiography can be performed using a catheter-deployable dual-frequency transducer to detect microvasculature through superharmonic imaging methods.Doctor of Philosoph

A device for performing sonoporation on adherent cell cultures

Sonoporation is a method for inducing a transient increase in the permeability of cell membranes to otherwise impermeable compounds using ultrasound. This technique has therapeutic potential as it allows for localized delivery of therapeutic agents in a noninvasive and non-cytotoxic manner. The discovery and testing of potential therapeutic agents that can be delivered using this technique requires performing studies on cell cultures in vitro. This thesis presents a prototype sonoporation device which aims to reduce the time and expertise required to perform sonoporation on adherent monolayer cell cultures. First, a prototype sonoporation device was designed and constructed. The device consisted of an array of six ultrasound transducers a xed below a cell culture stage. The six transducers were each constructed and electrically matched to 50 at an operating frequency of 1 MHz. The acoustic near- eld of each transducer was characterized using hydrophone scanning and the distance from the transducer at which the plane perpendicular to the beam path was most homogeneous was determined. The mean( s.d.) treatment distance was 15.9( 0.67) mm and the mean -3 dB width was 1.97( 0.22) mm. The electrical power required to produce 0.7 MPa on this plane was found for each transducer. The mean( s.d.) electrical power was 101( 12.2) W. Next, the prototype device was experimentally validated. Sonoporation was performed on cervical carcinoma-derived SiHa cells with 70-80% con uency at media temperatures of 37°C, 39.5°C, and 42°C. Pulsed ultrasound of 1 MHz, 4.8% duty cycle, 1.6 kHz pulse repetition frequency, and 0.7 MPa peak pressure was applied to induce sonoporation. Ultrasound contrast agent was added to the cell culture media (0.33% v/v) to provide cavitation nuclei during treatment. Plasmid DNA expressing green uorescent protein (GFP) was added to the cell culture (250 g/10 mL) to quantify successful permeabilization. While there were no signi cant e ects due to the temperature of the media, transfection was successfully performed using the prototype device given the positive expression of GFP in the cells 24 hours following treatment. The mean( s.d.) transfection e ciencies of the sonoporation treatment at 37°C, 39.5°C, and 42°C were 5.4( 0.92)%, 5.8( 1.3)%, and 5.3( 1.1)% respectively (n = 3 for each experimental group). Negative control treatments had transfection rates of < 1:5% on average and the detected levels of apoptosis among surviving cells was < 0:5% on average for all treatment groups. These results were in good agreement with those obtained using a di erent sonoporation experimental set-up on the same cell line with similar experimental parameters. Finally, the design of high-power ultrasound driving circuitry was explored in order to create an electrical device with the ability to provide independent, concurrent, and controlled excitation of the six transducers. A class DE half-bridge ampli er topology was chosen as the output power stage of this device. A design of a class DE ampli er was simulated using LTSpice with both a resistive 50 load and a Butterworth-Van Dyke equivalent circuit model of one of the six transducers, matched to 50 at 1 MHz. The ampli er was designed to deliver 150 W to a 50 resistive load at an output frequency of 1 MHz using a DC supply voltage of 96 V. The simulation of the ampli er using the transducer equivalent circuit yielded an output power of 134 W, a drain e ciency of 98.8%, a power-added e ciency of 89.0%, a gate power gain of 22.6 dB, and a total harmonic distortion at the output of 27.9%. The device presented here was shown to be e ective at performing sonoporation on adherent monolayer cell cultures and will reduce the time and expertise required to perform this technique in the future

Research on the propagation efficiency of ultrasonic guided waves in the rail

Ultrasonic guided waves (UGW) technique has the advantages of low detection frequency, long detection distance, strong anti-electromagnetic interference ability, and large coverage. Hence it has potential advantages in real-time detection of breakages in the rail. Based on the research background of UGW-based broken rail detection, this paper focuses on the characteristics optimization of piezoelectric ultrasonic transducers (PUTs) to improve the propagation efficiency of UGW in the rail. Due to the influence of energy attenuation, multimodal, dispersion, and on-site noise when the UGW propagates in the rail, the amplitude of the received UGW signal is low and the signal-to-noise ratio is poor. Therefore, this thesis mainly systematically studies the characteristics optimization of PUTs from the aspects of impedance matching, driving circuit optimization, and excitation signal optimization. The main work is as follows: 1. To deeply study of the electromechanical characteristics of longitudinal vibration sandwich piezoelectric ultrasonic transducer (referred to as PUTs), the PSpice equivalent circuit models of a piezoelectric ultrasonic transducer and the PSpice equivalent circuit model of a pitch-catch setup are established based on one-dimensional wave and transmission line theory. The PSpice model of the PUT and the PSpice model of the pitch-catch setup are analyzed from the time and frequency domains, respectively, and the accuracy of the built PSpice models is verified through some experiments. It is shown that the PSpice model of a PUT established above is highly scalable and can be combined with amplifiers, driving circuits, diodes. 2. With the aim of solving the problem of impedance mismatch between the piezoelectric ultrasonic transducer and the driving circuit and the rail surface, the effect of the impedance matching on the electromechanical properties of the piezoelectric ultrasonic transducer was studied from the electrical and acoustic ends, respectively. From the electrical side, the effects of different electrical impedance matching networks on the electromechanical characteristics of PUTs are studied in both time and frequency domains. It is shown that in the two LC impedance matching networks, the matching network formed by the series inductance and parallel capacitance is better. From the acoustic side, an experimental method is used to study the effect of acoustic impedance matching on the transient characteristics of PUTs. It is concluded that when the epoxy resin is doped with 10% tungsten powder and the coating thickness is 8 mm, the acoustic impedance matching effect is better. 3. To overcome the problems of the existing driving circuits that the excitation voltage is not high enough, the extra high voltage DC voltage is required and the impedance matching is not considered, this thesis proposed a high voltage pulse driving circuit based on the full-bridge topology. The driving circuit takes into account the suppression of overshoot and oscillation when the power MOSFET is turned off, and at the same time conducts the impedance matching and tailing absorption of the excitation signal for PUTs. The suppression of overshoot and oscillation adopts the RC snubber circuit, and the tailing absorption is accomplished by a bleeder resistor and a bidirectional thyristor. The correctness and effectiveness of the proposed high-voltage pulse driving circuit are verified through experiments. It was also found that the combined use of electrical impedance matching and absorption circuits can effectively improve the energy conversion efficiency of PUTs. 4. To obtain the optimal performance of PUTs, the excitation signal of PUTs is optimized in terms of excitation signal frequency and excitation coding. First of all, to solve the problem of PUTs with having a resonance frequency shift after loading, this thesis proposes an optimal excitation frequency tracking method based on a digital band-pass tracking filtering. Then its correctness and stability are verified through some field experiments. Secondly, to improve the signal-to-noise ratio of the UGW signal, it is proposed to apply the Barker code excitation method to the broken rail detection, and use the pulse compression technique at the receiving end to realize the rapid recognition of the signal characteristics. Finally, for the case where the pulse-compressed signal produces undesirable peak sidelobes due to the effects of bandwidth, multipath, and noise, an adaptive peak detection algorithm based on the Hilbert transform combined with a digital bandpass tracking filter and a triangle filter. The accuracy and effectiveness of the above-mentioned Barker code excitation method and the adaptive peak detection algorithm are verified through experiments. The study in this thesis presents a feasible solution for improving the propagation efficiency of UGW in the rails and at the same time provides theoretical guidance for the large-scale application of the real-time broken rail detection system based on UGW

Navigation system based in motion tracking sensor for percutaneous renal access

Tese de Doutoramento em Engenharia BiomédicaMinimally-invasive kidney interventions are daily performed to diagnose and treat several renal diseases. Percutaneous renal access (PRA) is an essential but challenging stage for most of these procedures, since its outcome is directly linked to the physician’s ability to precisely visualize and reach the anatomical target. Nowadays, PRA is always guided with medical imaging assistance, most frequently using X-ray based imaging (e.g. fluoroscopy). Thus, radiation on the surgical theater represents a major risk to the medical team, where its exclusion from PRA has a direct impact diminishing the dose exposure on both patients and physicians. To solve the referred problems this thesis aims to develop a new hardware/software framework to intuitively and safely guide the surgeon during PRA planning and puncturing. In terms of surgical planning, a set of methodologies were developed to increase the certainty of reaching a specific target inside the kidney. The most relevant abdominal structures for PRA were automatically clustered into different 3D volumes. For that, primitive volumes were merged as a local optimization problem using the minimum description length principle and image statistical properties. A multi-volume Ray Cast method was then used to highlight each segmented volume. Results show that it is possible to detect all abdominal structures surrounding the kidney, with the ability to correctly estimate a virtual trajectory. Concerning the percutaneous puncturing stage, either an electromagnetic or optical solution were developed and tested in multiple in vitro, in vivo and ex vivo trials. The optical tracking solution aids in establishing the desired puncture site and choosing the best virtual puncture trajectory. However, this system required a line of sight to different optical markers placed at the needle base, limiting the accuracy when tracking inside the human body. Results show that the needle tip can deflect from its initial straight line trajectory with an error higher than 3 mm. Moreover, a complex registration procedure and initial setup is needed. On the other hand, a real-time electromagnetic tracking was developed. Hereto, a catheter was inserted trans-urethrally towards the renal target. This catheter has a position and orientation electromagnetic sensor on its tip that function as a real-time target locator. Then, a needle integrating a similar sensor is used. From the data provided by both sensors, one computes a virtual puncture trajectory, which is displayed in a 3D visualization software. In vivo tests showed a median renal and ureteral puncture times of 19 and 51 seconds, respectively (range 14 to 45 and 45 to 67 seconds). Such results represent a puncture time improvement between 75% and 85% when comparing to state of the art methods. 3D sound and vibrotactile feedback were also developed to provide additional information about the needle orientation. By using these kind of feedback, it was verified that the surgeon tends to follow a virtual puncture trajectory with a reduced amount of deviations from the ideal trajectory, being able to anticipate any movement even without looking to a monitor. Best results show that 3D sound sources were correctly identified 79.2 ± 8.1% of times with an average angulation error of 10.4º degrees. Vibration sources were accurately identified 91.1 ± 3.6% of times with an average angulation error of 8.0º degrees. Additionally to the EMT framework, three circular ultrasound transducers were built with a needle working channel. One explored different manufacture fabrication setups in terms of the piezoelectric materials, transducer construction, single vs. multi array configurations, backing and matching material design. The A-scan signals retrieved from each transducer were filtered and processed to automatically detect reflected echoes and to alert the surgeon when undesirable anatomical structures are in between the puncture path. The transducers were mapped in a water tank and tested in a study involving 45 phantoms. Results showed that the beam cross-sectional area oscillates around the ceramics radius and it was possible to automatically detect echo signals in phantoms with length higher than 80 mm. Hereupon, it is expected that the introduction of the proposed system on the PRA procedure, will allow to guide the surgeon through the optimal path towards the precise kidney target, increasing surgeon’s confidence and reducing complications (e.g. organ perforation) during PRA. Moreover, the developed framework has the potential to make the PRA free of radiation for both patient and surgeon and to broad the use of PRA to less specialized surgeons.Intervenções renais minimamente invasivas são realizadas diariamente para o tratamento e diagnóstico de várias doenças renais. O acesso renal percutâneo (ARP) é uma etapa essencial e desafiante na maior parte destes procedimentos. O seu resultado encontra-se diretamente relacionado com a capacidade do cirurgião visualizar e atingir com precisão o alvo anatómico. Hoje em dia, o ARP é sempre guiado com recurso a sistemas imagiológicos, na maior parte das vezes baseados em raios-X (p.e. a fluoroscopia). A radiação destes sistemas nas salas cirúrgicas representa um grande risco para a equipa médica, aonde a sua remoção levará a um impacto direto na diminuição da dose exposta aos pacientes e cirurgiões. De modo a resolver os problemas existentes, esta tese tem como objetivo o desenvolvimento de uma framework de hardware/software que permita, de forma intuitiva e segura, guiar o cirurgião durante o planeamento e punção do ARP. Em termos de planeamento, foi desenvolvido um conjunto de metodologias de modo a aumentar a eficácia com que o alvo anatómico é alcançado. As estruturas abdominais mais relevantes para o procedimento de ARP, foram automaticamente agrupadas em volumes 3D, através de um problema de optimização global com base no princípio de “minimum description length” e propriedades estatísticas da imagem. Por fim, um procedimento de Ray Cast, com múltiplas funções de transferência, foi utilizado para enfatizar as estruturas segmentadas. Os resultados mostram que é possível detetar todas as estruturas abdominais envolventes ao rim, com a capacidade para estimar corretamente uma trajetória virtual. No que diz respeito à fase de punção percutânea, foram testadas duas soluções de deteção de movimento (ótica e eletromagnética) em múltiplos ensaios in vitro, in vivo e ex vivo. A solução baseada em sensores óticos ajudou no cálculo do melhor ponto de punção e na definição da melhor trajetória a seguir. Contudo, este sistema necessita de uma linha de visão com diferentes marcadores óticos acoplados à base da agulha, limitando a precisão com que a agulha é detetada no interior do corpo humano. Os resultados indicam que a agulha pode sofrer deflexões à medida que vai sendo inserida, com erros superiores a 3 mm. Por outro lado, foi desenvolvida e testada uma solução com base em sensores eletromagnéticos. Para tal, um cateter que integra um sensor de posição e orientação na sua ponta, foi colocado por via trans-uretral junto do alvo renal. De seguida, uma agulha, integrando um sensor semelhante, é utilizada para a punção percutânea. A partir da diferença espacial de ambos os sensores, é possível gerar uma trajetória de punção virtual. A mediana do tempo necessário para puncionar o rim e ureter, segundo esta trajetória, foi de 19 e 51 segundos, respetivamente (variações de 14 a 45 e 45 a 67 segundos). Estes resultados representam uma melhoria do tempo de punção entre 75% e 85%, quando comparados com o estado da arte dos métodos atuais. Além do feedback visual, som 3D e feedback vibratório foram explorados de modo a fornecer informações complementares da posição da agulha. Verificou-se que com este tipo de feedback, o cirurgião tende a seguir uma trajetória de punção com desvios mínimos, sendo igualmente capaz de antecipar qualquer movimento, mesmo sem olhar para o monitor. Fontes de som e vibração podem ser corretamente detetadas em 79,2 ± 8,1% e 91,1 ± 3,6%, com erros médios de angulação de 10.4º e 8.0 graus, respetivamente. Adicionalmente ao sistema de navegação, foram também produzidos três transdutores de ultrassom circulares com um canal de trabalho para a agulha. Para tal, foram exploradas diferentes configurações de fabricação em termos de materiais piezoelétricos, transdutores multi-array ou singulares e espessura/material de layers de suporte. Os sinais originados em cada transdutor foram filtrados e processados de modo a detetar de forma automática os ecos refletidos, e assim, alertar o cirurgião quando existem variações anatómicas ao longo do caminho de punção. Os transdutores foram mapeados num tanque de água e testados em 45 phantoms. Os resultados mostraram que o feixe de área em corte transversal oscila em torno do raio de cerâmica, e que os ecos refletidos são detetados em phantoms com comprimentos superiores a 80 mm. Desta forma, é expectável que a introdução deste novo sistema a nível do ARP permitirá conduzir o cirurgião ao longo do caminho de punção ideal, aumentado a confiança do cirurgião e reduzindo possíveis complicações (p.e. a perfuração dos órgãos). Além disso, de realçar que este sistema apresenta o potencial de tornar o ARP livre de radiação e alarga-lo a cirurgiões menos especializados.The present work was only possible thanks to the support by the Portuguese Science and Technology Foundation through the PhD grant with reference SFRH/BD/74276/2010 funded by FCT/MEC (PIDDAC) and by Fundo Europeu de Desenvolvimento Regional (FEDER), Programa COMPETE - Programa Operacional Factores de Competitividade (POFC) do QREN

Noninvasive Thrombolysis Using Histotripsy Pulsed Ultrasound Cavitation Therapy.

Histotripsy is a noninvasive ultrasound therapy that utilizes short, high-amplitude, focused ultrasound pulses to mechanically reduce targeted tissue structures to liquid debris by acoustic cavitation. In this work, the physical mechanisms of histotripsy and its application as a method of thrombolysis were investigated. Cavitation activity which causes tissue breakdown during histotripsy was studied by high-speed photography. It was found that cavitation clouds form due to scattering of shock waves in a focused ultrasound pulse from individual inertial cavitation bubbles. The scattered shock is a large tensile wave which expands clusters of cavitation bubbles when the tensile pressure is greater than a measured threshold of approximately 30 MPa. The interaction of this cavitation with tissue and cells was explored with a phantom containing agarose and red blood cells to measure cavitation-based mechanical damage. The observations indicated that cell lysis may be achieved by bubble-induced tensile strain upon expansion, causing membrane rupture. Based on these studies, focused histotripsy therapy transducers were designed to controllably generate cavitation clouds in the vasculature for performing thrombolysis. Transducers were integrated with ultrasound imagers to provide feedback for targeting and monitoring progress of treatment. Rapid thrombolysis was observed when histotripsy was applied to clots in-vitro, and the resulting debris was mainly subcellular and unlikely to cause embolism. Additionally, it was observed that histotripsy can attract, trap, and destroy free clot fragments in a vessel phantom. Based on these observations, a noninvasive embolus trap (NET) was developed, acting as a filter to prevent embolism during the thrombolysis procedure. An in-vivo porcine model of deep-vein thrombosis was used to evaluate the safety and efficacy of the histotripsy thrombolysis technique. These experiments demonstrated the feasibility of the treatment and suggest histotripsy can achieve rapid clot breakdown in a controlled manner.Ph.D.Biomedical EngineeringUniversity of Michigan, Horace H. Rackham School of Graduate Studieshttp://deepblue.lib.umich.edu/bitstream/2027.42/91496/1/adamdm_1.pd