115,729 research outputs found
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A conserved morphogenetic mechanism for epidermal ensheathment of nociceptive sensory neurites.
Interactions between epithelial cells and neurons influence a range of sensory modalities including taste, touch, and smell. Vertebrate and invertebrate epidermal cells ensheath peripheral arbors of somatosensory neurons, including nociceptors, yet the developmental origins and functional roles of this ensheathment are largely unknown. Here, we describe an evolutionarily conserved morphogenetic mechanism for epidermal ensheathment of somatosensory neurites. We found that somatosensory neurons in Drosophila and zebrafish induce formation of epidermal sheaths, which wrap neurites of different types of neurons to different extents. Neurites induce formation of plasma membrane phosphatidylinositol 4,5-bisphosphate microdomains at nascent sheaths, followed by a filamentous actin network, and recruitment of junctional proteins that likely form autotypic junctions to seal sheaths. Finally, blocking epidermal sheath formation destabilized dendrite branches and reduced nociceptive sensitivity in Drosophila. Epidermal somatosensory neurite ensheathment is thus a deeply conserved cellular process that contributes to the morphogenesis and function of nociceptive sensory neurons
Flora robotica -- An Architectural System Combining Living Natural Plants and Distributed Robots
Key to our project flora robotica is the idea of creating a bio-hybrid system
of tightly coupled natural plants and distributed robots to grow architectural
artifacts and spaces. Our motivation with this ground research project is to
lay a principled foundation towards the design and implementation of living
architectural systems that provide functionalities beyond those of orthodox
building practice, such as self-repair, material accumulation and
self-organization. Plants and robots work together to create a living organism
that is inhabited by human beings. User-defined design objectives help to steer
the directional growth of the plants, but also the system's interactions with
its inhabitants determine locations where growth is prohibited or desired
(e.g., partitions, windows, occupiable space). We report our plant species
selection process and aspects of living architecture. A leitmotif of our
project is the rich concept of braiding: braids are produced by robots from
continuous material and serve as both scaffolds and initial architectural
artifacts before plants take over and grow the desired architecture. We use
light and hormones as attraction stimuli and far-red light as repelling
stimulus to influence the plants. Applied sensors range from simple proximity
sensing to detect the presence of plants to sophisticated sensing technology,
such as electrophysiology and measurements of sap flow. We conclude by
discussing our anticipated final demonstrator that integrates key features of
flora robotica, such as the continuous growth process of architectural
artifacts and self-repair of living architecture.Comment: 16 pages, 12 figure
Predict or classify: The deceptive role of time-locking in brain signal classification
Several experimental studies claim to be able to predict the outcome of
simple decisions from brain signals measured before subjects are aware of their
decision. Often, these studies use multivariate pattern recognition methods
with the underlying assumption that the ability to classify the brain signal is
equivalent to predict the decision itself. Here we show instead that it is
possible to correctly classify a signal even if it does not contain any
predictive information about the decision. We first define a simple stochastic
model that mimics the random decision process between two equivalent
alternatives, and generate a large number of independent trials that contain no
choice-predictive information. The trials are first time-locked to the time
point of the final event and then classified using standard machine-learning
techniques. The resulting classification accuracy is above chance level long
before the time point of time-locking. We then analyze the same trials using
information theory. We demonstrate that the high classification accuracy is a
consequence of time-locking and that its time behavior is simply related to the
large relaxation time of the process. We conclude that when time-locking is a
crucial step in the analysis of neural activity patterns, both the emergence
and the timing of the classification accuracy are affected by structural
properties of the network that generates the signal.Comment: 23 pages, 5 figure
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Indoor acids and bases.
Numerous acids and bases influence indoor air quality. The most abundant of these species are CO2 (acidic) and NH3 (basic), both emitted by building occupants. Other prominent inorganic acids are HNO3 , HONO, SO2 , H2 SO4 , HCl, and HOCl. Prominent organic acids include formic, acetic, and lactic; nicotine is a noteworthy organic base. Sources of N-, S-, and Cl-containing acids can include ventilation from outdoors, indoor combustion, consumer product use, and chemical reactions. Organic acids are commonly more abundant indoors than outdoors, with indoor sources including occupants, wood, and cooking. Beyond NH3 and nicotine, other noteworthy bases include inorganic and organic amines. Acids and bases partition indoors among the gas-phase, airborne particles, bulk water, and surfaces; relevant thermodynamic parameters governing the partitioning are the acid-dissociation constant (Ka ), Henry's law constant (KH ), and the octanol-air partition coefficient (Koa ). Condensed-phase water strongly influences the fate of indoor acids and bases and is also a medium for chemical interactions. Indoor surfaces can be large reservoirs of acids and bases. This extensive review of the state of knowledge establishes a foundation for future inquiry to better understand how acids and bases influence the suitability of indoor environments for occupants, cultural artifacts, and sensitive equipment
Expression of A152T human tau causes age-dependent neuronal dysfunction and loss in transgenic mice.
A152T-variant human tau (hTau-A152T) increases risk for tauopathies, including Alzheimer's disease. Comparing mice with regulatable expression of hTau-A152T or wild-type hTau (hTau-WT), we find age-dependent neuronal loss, cognitive impairments, and spontaneous nonconvulsive epileptiform activity primarily in hTau-A152T mice. However, overexpression of either hTau species enhances neuronal responses to electrical stimulation of synaptic inputs and to an epileptogenic chemical. hTau-A152T mice have higher hTau protein/mRNA ratios in brain, suggesting that A152T increases production or decreases clearance of hTau protein. Despite their functional abnormalities, aging hTau-A152T mice show no evidence for accumulation of insoluble tau aggregates, suggesting that their dysfunctions are caused by soluble tau. In human amyloid precursor protein (hAPP) transgenic mice, co-expression of hTau-A152T enhances risk of early death and epileptic activity, suggesting copathogenic interactions between hTau-A152T and amyloid-β peptides or other hAPP metabolites. Thus, the A152T substitution may augment risk for neurodegenerative diseases by increasing hTau protein levels, promoting network hyperexcitability, and synergizing with the adverse effects of other pathogenic factors
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Dispersal in microbes: fungi in indoor air are dominated by outdoor air and show dispersal limitation at short distances.
The indoor microbiome is a complex system that is thought to depend on dispersal from the outdoor biome and the occupants' microbiome combined with selective pressures imposed by the occupants' behaviors and the building itself. We set out to determine the pattern of fungal diversity and composition in indoor air on a local scale and to identify processes behind that pattern. We surveyed airborne fungal assemblages within 1-month time periods at two seasons, with high replication, indoors and outdoors, within and across standardized residences at a university housing facility. Fungal assemblages indoors were diverse and strongly determined by dispersal from outdoors, and no fungal taxa were found as indicators of indoor air. There was a seasonal effect on the fungi found in both indoor and outdoor air, and quantitatively more fungal biomass was detected outdoors than indoors. A strong signal of isolation by distance existed in both outdoor and indoor airborne fungal assemblages, despite the small geographic scale in which this study was undertaken (<500 m). Moreover, room and occupant behavior had no detectable effect on the fungi found in indoor air. These results show that at the local level, outdoor air fungi dominate the patterning of indoor air. More broadly, they provide additional support for the growing evidence that dispersal limitation, even on small geographic scales, is a key process in structuring the often-observed distance-decay biogeographic pattern in microbial communities
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