On the Stabilizing Action of Protein Denaturants: Acetonitrile Effect on Stability of Lysozyme in Aqueous Solutions

Stability of hen lysozyme in the presence of acetonitrile (MeCN) at different pH values of the medium was studied by scanning microcalorimetry with a special emphasis on determination of reliable values of the denaturational heat capacity change. It was found that the temperature of denaturation decreases on addition of MeCN. However, the free energy extrapolation showed that below room temperature the thermodynamic stability increases at low concentrations of MeCN in spite of the general destabilizing effect at higher concentrations and temperatures. Charge-induced contribution to this stabilization was shown to be negligible (no pH-dependence was found); therefore, the most probable cause for the phenomenon is an increase of hydrophobic interactions at low temperatures in aqueous solutions containing small amounts of the organic additive. The difference in preferential solvation of native and denatured states of lysozyme was calculated from the stabilization free energy data. It was found that the change in preferential solvation strongly depends on the temperature in the water-rich region. At the higher MeCN content this dependence decreases until, at 0.06 mole fractions of MeCN, the difference in the preferential solvation between native and denatured lysozyme becomes independent of the temperature over a range of 60 K. The importance of taking into account non-ideality of a mixed solution, when analyzing preferential solvation phenomena was emphasized

Chitosan-cellulose Composite Materials: Preparation, Characterization and Application for Removal of Microcystin

We developed a simple and one-step method to prepare biocompatible composites from cellulose (CEL) and chitosan (CS). [BMIm+Cl−], an ionic liquid (IL), was used as a green solvent to dissolve and prepare the [CEL + CS] composites. Since majority (\u3e88%) of IL used was recovered for reuse by distilling the aqueous washings of [CEL + CS], the method is recyclable. XRD, FTIR, NIR, 13C CP-MAS-NMR and SEM were used to monitor the dissolution and to characterize the composites. The composite was found to have combined advantages of their components: superior mechanical strength (from CEL) and excellent adsorption capability for microcystin-LR, a deadly toxin produced by cyanobacteria (from CS). Specifically, the mechanical strength of the composites increased with CEL loading; e.g., up to 5× increase in tensile strength was achieved by adding 80% of CEL into CS. Kinetic results of adsorption confirm that unique properties of CS remain intact in the composite, i.e., it is not only a very good adsorbent for microcystin but also is better than all other available adsorbents. For example, it can adsorb 4× times more microcystin than the best reported adsorbent. Importantly, the microcystin adsorbed can be quantitatively desorbed to enable the composite to be reused with similar adsorption efficiency

On the variations of the elemental composition of the P3 component in presolar diamonds

Analysis of noble gas element variations in the P3 component of presolar diamonds indicates that a special mechanism of element fractionation is required to explain the variations

Presolar diamonds in Krymka: C, N and Xe isotope data from grain-size separates and comparison with other meteorites

Comparison of carbon isotope variations in grain size fractions of presolar diamonds separated from three meteorites (Efremovka, Boriskino and Krymka) suggest that diamond from various parts of solar nebular have had diffrent C isotope signature

Frontier exploration and the North Atlantic Igneous Province : new insights from a 2.6 km offshore volcanic sequence in the NE Faroe–Shetland Basin

Acknowledgements and Funding This work was funded by Chevron. The authors would like to acknowledge the Chevron West of Shetlands team along with the Joint Venture partners OMV, Faroe Petroleum and Indemitsu for access to data along with permission to publish this study. PGS is thanked for access to the Corona Ridge Regional Geostreamer (CRRG) data and permission to publish the seismic line. The paper was improved thanks to insightful reviews by S. M. Jones and A. Saunders, which substantially improved an earlier draft. J. Still and F. Thompson gave invaluable technical support at the University of Aberdeen, and K. Wall helped with real-time cuttings analysis.Peer reviewedPostprin

Automating the Composition of Middleware Configurations

A method is presented for the automatic construction of all possible valid compositions of different middleware software architectures. This allows reusing the latter in order to create systems providing a set of different non-functional properties. These compositions are constructed by using only the structural information of the architectures, i.e. their configurations. Yet, they provide a valuable insight on the different properties of the class of systems that can be constructed when a particular set of non-functional properties is required

Towards a Security, Privacy, Dependability, Interoperability Framework for the Internet of Things

A popular application of ambient intelligence systems constitutes of assisting living services on smart buildings. As intelligence is imported in embedded equipment, the system becomes able to provide smart services (e.g. control lights, airconditioning, provide energy management services etc.). IoT is the main enabler of such environments. However, the interconnection of these cyber-physical systems and the processing of personal data raise serious security and privacy issues. In this paper we present a framework that can guarantee Security, Privacy, Dependability and Interoperability (SPDI) in IoT. Taking advantage of the underlying IoT deployment, the proposed framework not only implements the requested smart functionality but also provide modelling and administration that can guarantee those SPDI properties. Moreover, we provide an application example of the framework in a smart building scenario