531 research outputs found
A neural circuit for navigation inspired by C. elegans Chemotaxis
We develop an artificial neural circuit for contour tracking and navigation
inspired by the chemotaxis of the nematode Caenorhabditis elegans. In order to
harness the computational advantages spiking neural networks promise over their
non-spiking counterparts, we develop a network comprising 7-spiking neurons
with non-plastic synapses which we show is extremely robust in tracking a range
of concentrations. Our worm uses information regarding local temporal gradients
in sodium chloride concentration to decide the instantaneous path for foraging,
exploration and tracking. A key neuron pair in the C. elegans chemotaxis
network is the ASEL & ASER neuron pair, which capture the gradient of
concentration sensed by the worm in their graded membrane potentials. The
primary sensory neurons for our network are a pair of artificial spiking
neurons that function as gradient detectors whose design is adapted from a
computational model of the ASE neuron pair in C. elegans. Simulations show that
our worm is able to detect the set-point with approximately four times higher
probability than the optimal memoryless Levy foraging model. We also show that
our spiking neural network is much more efficient and noise-resilient while
navigating and tracking a contour, as compared to an equivalent non-spiking
network. We demonstrate that our model is extremely robust to noise and with
slight modifications can be used for other practical applications such as
obstacle avoidance. Our network model could also be extended for use in
three-dimensional contour tracking or obstacle avoidance
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Olfaction-enhanced multimedia: Perspectives and challenges
This is the post-print version of the Article. The official published version can be accessed from the link below - Copyright @ 2011 Springer VerlagOlfaction—or smell—is one of the last challenges which multimedia and multimodal applications have to conquer. Enhancing such applications with olfactory stimuli has the potential to create a more complex—and richer—user multimedia experience, by heightening the sense of reality and diversifying user interaction modalities. Nonetheless, olfaction-enhanced multimedia still remains a challenging research area. More recently, however, there have been initial signs of olfactory-enhanced applications in multimedia, with olfaction being used towards a variety of goals, including notification alerts, enhancing the sense of reality in immersive applications, and branding, to name but a few. However, as the goal of a multimedia application is to inform and/or entertain users, achieving quality olfaction-enhanced multimedia applications from the users’ perspective is vital to the success and continuity of these applications. Accordingly, in this paper we have focused on investigating the user perceived experience of olfaction-enhanced multimedia applications, with the aim of discovering the quality evaluation factors that are important from a user’s perspective of these applications, and consequently ensure the continued advancement and success of olfaction-enhanced multimedia applications
Microbial light-activatable proton pumps as neuronal inhibitors to functionally dissect neuronal networks in C. elegans
Essentially any behavior in simple and complex animals depends on neuronal network function. Currently, the best-defined system to study neuronal circuits is the nematode Caenorhabditis elegans, as the connectivity of its 302 neurons is exactly known. Individual neurons can be activated by photostimulation of Channelrhodopsin-2 (ChR2) using blue light, allowing to directly probe the importance of a particular neuron for the respective behavioral output of the network under study. In analogy, other excitable cells can be inhibited by expressing Halorhodopsin from Natronomonas pharaonis (NpHR) and subsequent illumination with yellow light. However, inhibiting C. elegans neurons using NpHR is difficult. Recently, proton pumps from various sources were established as valuable alternative hyperpolarizers. Here we show that archaerhodopsin-3 (Arch) from Halorubrum sodomense and a proton pump from the fungus Leptosphaeria maculans (Mac) can be utilized to effectively inhibit excitable cells in C. elegans. Arch is the most powerful hyperpolarizer when illuminated with yellow or green light while the action spectrum of Mac is more blue-shifted, as analyzed by light-evoked behaviors and electrophysiology. This allows these tools to be combined in various ways with ChR2 to analyze different subsets of neurons within a circuit. We exemplify this by means of the polymodal aversive sensory ASH neurons, and the downstream command interneurons to which ASH neurons signal to trigger a reversal followed by a directional turn. Photostimulating ASH and subsequently inhibiting command interneurons using two-color illumination of different body segments, allows investigating temporal aspects of signaling downstream of ASH
C. elegans odour detection in the diagnosis of prostate cancer
There are about 200 distinct forms of cancer, each of which is diagnosed and treated differently. Early identification and treatment of cancer is especially important as the population ages and cases become more frequent. In Finland prostate cancer is the most prevalent cancer in men with approximately 5000 new cases and 900 deaths yearly. There are nearly 1,3 million cases globally every year. Despite advances in recent years, prostate cancer continues to be a significant medical issue for afflicted men, with overtreatment of benign illness and lack of effective medicines for metastatic prostate cancer.
Prostate cancer rarely displays symptoms until it is incurable. Present technologies are unable to reliably identify between those tumours that will advance so slowly that they will not generate symptoms and those that are likely to cause death. As there is no sure way to prevent prostate cancer, the only way to reduce suffering and mortality is via early identification and competent patient care. Early detection followed by appropriate treatment plays a big role in the recovery while economical and accessible diagnosis methods are of vital importance.
Presently prostate-specific antigen (PSA) screening is frequently employed for detecting prostate cancer in its early state. However, it is considered a controversial method, since around 67% of men with an elevated PSA level obtain a false positive result and are not diagnosed with prostate cancer, whereas 15% of men with a negative result will develop cancer.
The Caenorhabditis elegans nematodes are widely used in research as a model organism since they are easy to maintain and possess many well-defined features. These include an efficient chemosensory system, which allows C. elegans to distinguish between substances in its surroundings and move towards or away from them. The chemosensory neurons in the head of C. elegans express around 700 distinct G protein–coupled receptors (GPCRs) that are able to recognize soluble or volatile compounds (VOCs) from the urine and exhaled air of cancer patients. It is thought that cancer metabolism generates particular odorants that could be useful in the early detection of the disease.
The goal of this study was to determine how well the C. elegans nematodes can detect prostate cancer from human urine samples. Chemotaxis assays were performed with urine samples of men with either benign hyperplasia of the prostate or malignant prostate cancer. Results obtained during this study were fairly variable and not reliable enough to recommend the method for clinical use. However, several factors may have influenced the results that were not in line with previously published data. Thus, further studies on this topic are definitely needed
Tactile Roughness Perception in the Presence of Olfactory and Trigeminal Stimulants
Previous research has shown that odorants consistently evoke associations with textures and their tactile properties like smoothness and roughness. Also, it has been observed that olfaction can modulate tactile perception. We therefore hypothesized that tactile roughness perception may be biased towards the somatosensory connotation of an ambient odorant. We performed two experiments to test this hypothesis. In the first experiment, we investigated the influence of ambient chemosensory stimuli with different roughness connotations on tactile roughness perception. In addition to a pleasant odor with a connotation of softness (PEA), we also included a trigeminal stimulant with a rough, sharp or prickly connotation (Ethanol). We expected that - compared to a No - odorant control condition—tactile texture perception would be biased towards smoothness in the presence of PEA and towards roughness in the presence of Ethanol. However, our results show no significant interaction between chemosensory stimulation and perceived tactile surface roughness. It could be argued that ambient odors may be less effective in stimulating crossmodal associations, since they are by definition extraneous to the tactile stimuli. In an attempt to optimize the conditions for sensory integration, we therefore performed a second experiment in which the olfactory and tactile stimuli were presented in synchrony and in close spatial proximity. In addition, we included pleasant (Lemon) and unpleasant (Indole) odorants that are known to have the ability to affect tactile perception. We expected that tactile stimuli would be perceived as less rough when simultaneously presented with Lemon or PEA (both associated with softness) than when presented with Ethanol or Indole (odors that can be associated with roughness). Again, we found no significant main effect of chemosensory condition on perceived tactile roughness. We discuss the limitations of this study and we present suggestions for future research.Previous research has shown that odorants consistently evoke associations with textures and their tactile properties like smoothness and roughness. Also, it has been observed that olfaction can modulate tactile perception. We therefore hypothesized that tactile roughness perception may be biased towards the somatosensory connotation of an ambient odorant. We performed two experiments to test this hypothesis. In the first experiment, we investigated the influence of ambient chemosensory stimuli with different roughness connotations on tactile roughness perception. In addition to a pleasant odor with a connotation of softness (PEA), we also included a trigeminal stimulant with a rough, sharp or prickly connotation (Ethanol). We expected that—compared to a No-odorant control condition—tactile texture perception would be biased towards smoothness in the presence of PEA and towards roughness in the presence of Ethanol. However, our results show no significant interaction between chemosensory stimulation and perceived tactile surface roughness. It could be argued that ambient odors may be less effective in stimulating crossmodal associations, since they are by definition extraneous to the tactile stimuli. In an attempt to optimize the conditions for sensory integration, we therefore performed a second experiment in which the olfactory and tactile stimuli were presented in synchrony and in close spatial proximity. In addition, we included pleasant (Lemon) and unpleasant (Indole) odorants that are known to have the ability to affect tactile perception. We expected that tactile stimuli would be perceived as less rough when simultaneously presented with Lemon or PEA (both associated with softness) than when presented with Ethanol or Indole (odors that can be associated with roughness). Again, we found no significant main effect of chemosensory condition on perceived tactile roughness. We discuss the limitations of this study and we present suggestions for future research
Olfaction: An Overlooked Sensory Modality in Applied Ethology and Animal Welfare
Birte L. Nielsen [et al.]Peer reviewe
Prevalence of olfactory dysfunction and quality of life in hospitalised patients 1 year after SARS-CoV-2 infection: a cohort study
Objectives: To determine the long-term prevalence of olfactory and/or gustatory dysfunction (OD±GD), associated risk factors and impact on quality of life (QoL) in previously hospitalised patients with COVID-19 1 year after infection. // Design: A single-centre cohort study. // Setting: Patients admitted at a large central London hospital with COVID-19 infection between 10 February 2020 and 22 May 2020. // Participants: 150 adult subjects with previously confirmed SARS-CoV-2 infection were recruited between 10 December 2020 and 29 January 2021. Participants were predominantly male (102/150, 68.0%); mean age 58.0±15.9 years and 41.2% (56/136) were of black, Asian and minority ethnic backgrounds. // Main outcome measures: EQ-5D-5L values and Sino-Nasal Outcome Test-22 (SNOT-22) scores. // Results: Long-term prevalence of OD±GD was 12.8% (19/149) at median time of 264.5 days following SARS-CoV-2 infection onset. Patients with OD±GD had a significantly higher median total SNOT-22 score (46.1; Q1–Q3: 23.0–60.0; 95% CI 23.0 to 60.0) compared with those without (16.0; Q1–Q3: 5.0–30.5; 95% CI 12.0 to 18.0) (p=0.0002), reflecting poorer QoL, particularly psychological well-being (p=0.0004), which was not alleviated with time (p=0.4977). Median EQ-5D-5L value was not significantly different between patients with OD±GD (0.70; Q1–Q3: 0.38–0.83; 95% CI 0.38 to 0.83) and those without (0.83; Q1–Q3: 0.61–0.94; 95% CI 0.75 to 0.89) (p=0.0627). Age, sex, ethnicity, smoking status, highest C reactive protein value, intubation and ventilation, and oxygen supplementation were not found to influence OD±GD (p>0.05). // Conclusions: 12.8% of previously hospitalised patients with COVID-19 in London still report persistent problems with smell or taste up to a year after infection, impacting their QoL. Increased holistic support including psychological therapy and olfactory rehabilitation for affected patients may help to reduce long-term morbidity
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Analysis and classification of drift susceptible chemosensory responses
textThis report presents machine learning models that can accurately classify gases by analyzing data from an array of 16 sensors. More specifically, the report presents basic decision tree models and advanced ensemble versions. The contribution of this report is to show that basic decision trees perform reasonably well on the gas sensor data, however their accuracy can be drastically improved by employing ensemble decision tree classifiers. The report presents bagged trees, Adaboost trees and Random Forest models in addition to basic entropy and Gini based trees. It is shown that ensemble classifiers achieve a very high degree of accuracy of 99% in classifying gases even when the sensor data is drift ridden. Finally, the report compares the accuracy of all the models developed.Electrical and Computer Engineerin
Nematophagous fungus Arthrobotrys oligospora mimics olfactory cues of sex and food to lure its nematode prey
To study the molecular basis for predator-prey coevolution, we investigated how Caenorhabditis elegans responds to the predatory fungus Arthrobotrys oligospora. C. elegans and other nematodes were attracted to volatile compounds produced by A. oligospora. Gas-chromatographic mass-spectral analyses of A. oligospora-derived volatile metabolites identified several odors mimicking food cues attractive to nematodes. One compound, methyl 3-methyl-2-butenoate (MMB) additionally triggered strong sex- and stage-specific attraction in several Caenorhabditis species. Furthermore, when MMB is present, it interferes with nematode mating, suggesting that MMB might mimic sex pheromone in Caenorhabditis species. Forward genetic screening suggests that multiple receptors are involved in sensing MMB. Response to fungal odors involves the olfactory neuron AWCs. Single-cell RNA-seq revealed the GPCRs expressed in AWC. We propose that A. oligospora likely evolved the means to use olfactory mimicry to attract its nematode prey through the olfactory neurons in C. elegans and related species
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