On the Porous Structuring using Unit Cells

Abstract This study presents the characteristics of the eleven commonly used porous structures. The structures are designed using ten different unit cells. Some of the unit cells consist of free-form surfaces (e.g., triply periodic minimal surface). Some of them are straightforward in design (e.g., honeycomb structure). Some of them have a hybrid structure. The 3D CAD models of the structures are created using commercially available CAD software. The finite element analysis is conducted for each structure to know how it behaves under a static load. The structures are also manufactured using a 3D printer to confirm the manufacturability of them. It is found that some of the structures are easy to manufacture, and some are not. Particularly, metal-alloy-printed structures need a minimal thickness. However, the structures' printed or virtual models are evaluated by determining their respective mass, production cost, production time, Mises stress, and surface area. Using the values of mass, production time and cost, Mises stress, and surface area, the optimal structure is identified. Thus, the outcomes of this study can help identify the optimal porous structure for a given purpose

Engineered three-dimensional scaffolds modulating fate of breast cancer cells using stiffness and morphology related cell adhesion

Goal: Artificially engineering the tumor microenvironment in vitro as a vital tool for understanding the mechanism of tumor progression. In this study, we developed three-dimensional cell scaffold systems with different topographical features and mechanical properties but similar surface chemistry. The cell behavior was modulated by the topography and mechanical properties of the scaffold. Adenocarcinoma (MCF7), triple-negative (MDA-MB-231) and premalignant (MCF10AneoT) breast cancer cells were seeded on the scaffold systems. The cell viability, cell-cell interaction and cell-matrix interactions were analyzed. The preferential growth and alignment of specific population of cells were demonstrated. Among the different scaffolds, triple-negative breast cancer cells preferred honeycomb scaffolds while adenocarcinoma cells favored mesh scaffolds and premalignant cells preferred the aligned scaffolds. The 3D model system developed here can be used to support growth of only specific cell populations or for the growth of tumors. This model can be used for understanding the topographical and mechanical features affecting tumorigenesis, cancer cell growth and migration behavior of malignant and metastatic cancer cells

Cell patterning via optimized dielectrophoretic force within hexagonal electrodes in vitro for skin tissue engineering

Abstract(#br)Tissue reconstruction through in vitro cell seeding is a popular method for tissue engineering. In this paper, we proposed a thin-layer structure consisting of multiple hexagons for the regeneration of skin tissue. Cells could be seeded and cultured within the structure via dielectrophoresis (DEP) actively. A thin layer of the structure was fabricated with biocompatible medical-grade stainless steel via precise laser cutting. The fabricated layers were stacked together to form a 3D electrode pair, which could be used to generate a 3D electric field. Thus, the suspended cells within the structure could be patterned via DEP manipulation. The input voltage was examined and optimized to ensure cell viability and patterning efficiency during the DEP manipulation process. As soon..

Characterization of a honeycomb-like scaffold with dielectrophoresis-based patterning for tissue engineering

202405 bcchAccepted ManuscriptRGCPublishedGreen (AAM

ARTIFICIAL SYNTHETIC SCAFFOLDS FOR TISSUE ENGINEERING APPLICATION EMPHASIZING THE ROLE OF BIOPHYSICAL CUES

The mechanotransduction of cells is the intrinsic ability of cells to convert the mechanical signals provided by the surrounding matrix and other cells into biochemical signals that affect several distinct processes such as tumorigenesis, wound healing, and organ formation. The use of biomaterials as an artificial scaffold for cell attachment, differentiation and proliferation provides a tool to modulate and understand the mechanotransduction pathways, develop better in vitro models and clinical remedies. The effect of topographical cues and stiffness was investigated in fibroblasts using polycaprolactone (PCL)- Polyaniline (PANI) based scaffolds that were fabricated using a self-assembly method and electrospinning. Through this method, scaffolds of different topography and stiffness were fabricated with similar surface chemistries. The effect of scaffold morphologies on the cells were investigated. PCL scaffolds of three distinct morphologies- honeycomb, aligned and mesh were used with similar surface chemistry to investigate the changes in cell behavior of breast, renal, lung and bladder cancer to the physical cues. Selective adhesion and localization of cells to specific morphologies were determined. In order to demonstrate the scaffold as a source of biochemical signals, ManCou-H, capable of targeting the fructose-specific glucose transporter GLUT5 was electrospun with the scaffolds of different morphologies. The PCL scaffolds were used as the backbone to release ManCou-H and changes in protein expression and metabolic activity was characterized. The findings made available through this research will help in the design of better cell-specific in vitro model systems to better understand cellular responses to clinical therapies, assess cell response to specific mechanical and chemical cues