47,783 research outputs found
Tractions and stress fibers control cell shape and rearrangements in collective cell migration
Key to collective cell migration is the ability of cells to rearrange their
position with respect to their neighbors. Recent theory and experiments
demonstrated that cellular rearrangements are facilitated by cell shape, with
cells having more elongated shapes and greater perimeters more easily sliding
past their neighbors within the cell layer. Though it is thought that cell
perimeter is controlled primarily by cortical tension and adhesion at each
cell's periphery, experimental testing of this hypothesis has produced
conflicting results. Here we studied collective cell migration in an epithelial
monolayer by measuring forces, cell perimeters, and motion, and found all three
to decrease with either increased cell density or inhibition of cell
contraction. In contrast to previous understanding, the data suggest that cell
shape and rearrangements are controlled not by cortical tension or adhesion at
the cell periphery but rather by the stress fibers that produce tractions at
the cell-substrate interface. This finding is confirmed by an experiment
showing that increasing tractions reverses the effect of density on cell shape
and rearrangements. Our study therefore reduces the focus on the cell periphery
by establishing cell-substrate traction as a major physical factor controlling
cell shape and motion in collective cell migration.Comment: 39 pages, 6 figure
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PAR-dependent and geometry-dependent mechanisms of spindle positioning.
During intrinsically asymmetric division, the spindle is oriented onto a polarized axis specified by a group of conserved PAR proteins. Extrinsic geometric asymmetry generated by cell shape also affects spindle orientation in some systems, but how intrinsic and extrinsic mechanisms coexist without interfering with each other is unknown. In some asymmetrically dividing cells of the wild-type Caenorhabditis elegans embryo, nuclear rotation directed toward the anterior cortex orients the forming spindle. We find that in such cells, a PAR-dependent mechanism dominates and causes rotation onto the polarized axis, regardless of cell shape. However, when geometric asymmetry is removed, free nuclear rotation in the center of the cell is observed, indicating that the anterior-directed nature of rotation in unaltered embryos is an effect of cell shape. This free rotation is inconsistent with the prevailing model for nuclear rotation, the specialized cortical site model. In contrast, in par-3 mutant embryos, a geometry-dependent mechanism becomes active and causes directed nuclear rotation. These results lead to the model that in wild-type embryos both PAR-3 and PAR-2 are essential for nuclear rotation in asymmetrically dividing cells, but that PAR-3 inhibits geometry-dependent rotation in nonpolarized cells, thus preventing cell shape from interfering with spindle orientation
The effect of flotation cell shape on deinking behaviour
Studies were undertaken to investigate the deinking behaviour of different shaped deinking cells of the same volume. For comparative purposes, most oprational variables were kept constant, and the same injector was used throughout the study. The position of the injector, however, was varied in some cases to go along with the particular cell shape being studied.
Three types of cell shapes were studied, (1) cylindrical with tangential air injection, (2) rectanular with vertical injection, and (3) rectangular with horizontal injection. Eucalyptus/toner slurries and news/mag wastepaper slurries were deinked. Flow patterns in the cells and the corresponding deinking efficiencies were measured.
It was found that strong and excessive re-circulatory flows within the cells could under certain conditions be a major factor in reducing brightness lift. Vertical injection into a rectangular cell gave stable flow patterns, non-wavy froth removal and sustained brightness lift for a wide range of feed and airflow rates. Horizontal injection into a similar rectangular shaped cell exhibited quite different characteristics. High brightness lift was possible for certain conditions and not for others. Wavy froth and excessive recirculation flow patterns varied with feed and airflow. The cylindrical cell with tangential injection gave stable circulatory flow and stable froth removal at low flow rates but was unable to deink at high flows
An atomic force microscope operating at hypergravity for in situ measurement of cellular mechano-response
We present a novel atomic force microscope (AFM) system, operational in liquid at variable gravity, dedicated to image cell shape changes of cells in vitro under hypergravity conditions. The hypergravity AFM is realized by mounting a stand-alone AFM into a large-diameter centrifuge. The balance between mechanical forces, both intra- and extracellular, determines both cell shape and integrity. Gravity seems to be an insignificant force at the level of a single cell, in contrast to the effect of gravity on a complete (multicellular) organism, where for instance bones and muscles are highly unloaded under near weightless (microgravity) conditions. However, past space flights and ground based cell biological studies, under both hypogravity and hypergravity conditions have shown changes in cell behaviour (signal transduction), cell architecture (cytoskeleton) and proliferation. Thus the role of direct or indirect gravity effects at the level of cells has remained unclear. Here we aim to address the role of gravity on cell shape. We concentrate on the validation of the novel AFM for use under hypergravity conditions. We find indications that a single cell exposed to 2 to 3 × g reduces some 30–50% in average height, as monitored with AFM. Indeed, in situ measurements of the effects of changing gravitational load on cell shape are well feasible by means of AFM in liquid. The combination provides a promising technique to measure, online, the temporal characteristics of the cellular mechano-response during exposure to inertial forces
Correlating Cell Shape and Cellular Stress in Motile Confluent Tissues
Collective cell migration is a highly regulated process involved in wound
healing, cancer metastasis and morphogenesis. Mechanical interactions among
cells provide an important regulatory mechanism to coordinate such collective
motion. Using a Self-Propelled Voronoi (SPV) model that links cell mechanics to
cell shape and cell motility, we formulate a generalized mechanical inference
method to obtain the spatio-temporal distribution of cellular stresses from
measured traction forces in motile tissues and show that such traction-based
stresses match those calculated from instantaneous cell shapes. We additionally
use stress information to characterize the rheological properties of the
tissue. We identify a motility-induced swim stress that adds to the interaction
stress to determine the global contractility or extensibility of epithelia. We
further show that the temporal correlation of the interaction shear stress
determines an effective viscosity of the tissue that diverges at the
liquid-solid transition, suggesting the possibility of extracting rheological
information directly from traction data.Comment: 12 pages, 9 figure
Rice microtubule‐associated protein IQ67‐DOMAIN14 regulates rice grain shape by modulating microtubule cytoskeleton dynamics
Cortical microtubule (MT) arrays play a critical role in plant cell shape determination by defining the direction of cell expansion. As plants continuously adapt to ever‐changing environmental conditions, multiple environmental and developmental inputs need to be translated into changes of the MT cytoskeleton. Here, we identify and functionally characterize an auxin‐inducible and MT‐localized protein OsIQ67‐DOMAIN14 (OsIQD14), which is highly expressed in rice seed hull cells. We show that while deficiency of OsIQD14 results in short and wide seeds and increases overall yield, overexpression leads to narrow and long seeds, caused by changed MT alignment. We further show that OsIQD14‐mediated MT reordering is regulated by specifically affecting MT dynamics, and ectopic expression of OsIQD14 in Arabidopsis could change the cell shape both in pavement cells and hypocotyl cells. Additionally, OsIQD14 activity is tightly controlled by calmodulin proteins, providing an alternative way to modify the OsIQD14 activity. Our results indicate that OsIQD14 acts as a key factor in regulating MT rearrangements in rice hull cells and hence the grain shape, and allows effective local cell shape manipulation to improve the rice yield trait
Cell shape analysis of random tessellations based on Minkowski tensors
To which degree are shape indices of individual cells of a tessellation
characteristic for the stochastic process that generates them? Within the
context of stochastic geometry and the physics of disordered materials, this
corresponds to the question of relationships between different stochastic
models. In the context of image analysis of synthetic and biological materials,
this question is central to the problem of inferring information about
formation processes from spatial measurements of resulting random structures.
We address this question by a theory-based simulation study of shape indices
derived from Minkowski tensors for a variety of tessellation models. We focus
on the relationship between two indices: an isoperimetric ratio of the
empirical averages of cell volume and area and the cell elongation quantified
by eigenvalue ratios of interfacial Minkowski tensors. Simulation data for
these quantities, as well as for distributions thereof and for correlations of
cell shape and volume, are presented for Voronoi mosaics of the Poisson point
process, determinantal and permanental point processes, and Gibbs hard-core and
random sequential absorption processes as well as for Laguerre tessellations of
polydisperse spheres and STIT- and Poisson hyperplane tessellations. These data
are complemented by mechanically stable crystalline sphere and disordered
ellipsoid packings and area-minimising foam models. We find that shape indices
of individual cells are not sufficient to unambiguously identify the generating
process even amongst this limited set of processes. However, we identify
significant differences of the shape indices between many of these tessellation
models. Given a realization of a tessellation, these shape indices can narrow
the choice of possible generating processes, providing a powerful tool which
can be further strengthened by density-resolved volume-shape correlations.Comment: Chapter of the forthcoming book "Tensor Valuations and their
Applications in Stochastic Geometry and Imaging" in Lecture Notes in
Mathematics edited by Markus Kiderlen and Eva B. Vedel Jense
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