Towards Bio-impedance Based Labs: A Review

In this article, some of the main contributions to BI (Bio-Impedance) parameter-based systems for medical, biological and industrial fields, oriented to develop micro laboratory systems are summarized. These small systems are enabled by the development of new measurement techniques and systems (labs), based on the impedance as biomarker. The electrical properties of the life mater allow the straightforward, low cost and usually non-invasive measurement methods to define its status or value, with the possibility to know its time evolution. This work proposes a review of bio-impedance based methods being employed to develop new LoC (Lab-on-a-Chips) systems, and some open problems identified as main research challenges, such as, the accuracy limits of measurements techniques, the role of the microelectrode-biological impedance modeling in measurements and system portability specifications demanded for many applications.Spanish founded Project: TEC 2013-46242-C3-1-P: Integrated Microsystem for Cell Culture AssaysFEDE

Development of a capacitive bioimpedance measurement system

Bioelectrical impedance spectroscopy (BIS) is a well-established and non-invasive method to determine and monitor body composition. Commercially available bioelectrical impedance systems use coated hydrogel-aluminium electrodes, where the hydrogel acts as an adhesive and as an electrolytic medium. The gel/adhesive is physiologically inert over short periods. However, when used over longer periods, hydrogel-aluminium electrodes present limitations, which capacitive electrodes may overcome. First measurements using capacitive electrodes have shown that commercial devices are not designed to work with these kind of electrodes. The presented high impedance, specially at low frequencies (e.g. 5kHz), presents a challenge for the current injection and therefore for the design of the current source. Within this project, a bioimpedance spectroscopy (BIS) system to perform measurements using capacitive electrodes has been developed. The system has been tested in the critical frequency range, namely in the lower frequency range (5 kHz - 43 kHz). Measurements have been performed using dummy electrical models, which simulate di erent values of skin and electrode impedance. The results obtained show the better performance of the device in comparison to a commercial device (Xitron Hydra 4200, Xitron Technologies) for that frequency range. An important item in this thesis has been the design of a multi-frequency current source able to perform measurements using capacitive electrodes

Noninvasive Acquisition of the Aortic Blood Pressure Waveform

Blood pressure reflects the status of our cardiovascular system. For the measurement of blood pressure, we typically use brachial devices on the upper arm, and much less often, the radial devices with pressure sensors on the wrist. Medical doctors know that this is an unfortunate case. The brachial pressure and even more, the radial pressure, both are poor replacements for the central aortic pressure (CAP). Moreover, the devices on the market cannot provide continuous measurements 24 h. In addition, most of the ambulatory and wearable monitors do not enable acquisition of the blood pressure curves in time. These circumstances limit the accuracy of diagnosing. The aim of this chapter is to introduce our experiments, experiences and results in developing the wearable monitor for central aortic blood pressure curve by using electrical bioimpedance sensing and measurement. First, electronic circuitry with embedded data acquisition and signal processing approaches is given. Second, finding appropriate materials, configurations and placements of electrodes is of interest. Third, the results of modelling and simulations are discussed for obtaining the best sensitivity and stability of the measurement procedures. Finally, the discussion on the provided provisional experiments evaluates the obtained results. The conclusions are drawn together with the need for further development

Multichannel bioimpedance measurement

This thesis is a research about the possibility of measure bioimpedance simultaneously in different body locations. It first rises a theoretical study facing the problem as a classic multichannel communication system with share channel, analysing the software and hardware problems involved for all the possibilities. The more suitable configuration chosen is based on frequency multiplexation, it consists of injecting isolated sine waves with different frequencies for each channel and uses a bandpass filter to get only the required one. About the signal processing the best option found is to record the signal using undersampling techniques and implements the filter digitally. Moreover a three channel test device has been made following the calculations for a eight channels one, in order to test the theoretical research and to find possible practical limitations. Finally this test device has been used for a concrete purpose, the breathing measuring in three points on the thorax

Development of a capacitive bioimpedance measurement system

Bioelectrical impedance spectroscopy (BIS) is a well-established and non-invasive method to determine and monitor body composition. Commercially available bioelectrical impedance systems use coated hydrogel-aluminium electrodes, where the hydrogel acts as an adhesive and as an electrolytic medium. The gel/adhesive is physiologically inert over short periods. However, when used over longer periods, hydrogel-aluminium electrodes present limitations, which capacitive electrodes may overcome. First measurements using capacitive electrodes have shown that commercial devices are not designed to work with these kind of electrodes. The presented high impedance, specially at low frequencies (e.g. 5kHz), presents a challenge for the current injection and therefore for the design of the current source. Within this project, a bioimpedance spectroscopy (BIS) system to perform measurements using capacitive electrodes has been developed. The system has been tested in the critical frequency range, namely in the lower frequency range (5 kHz - 43 kHz). Measurements have been performed using dummy electrical models, which simulate di erent values of skin and electrode impedance. The results obtained show the better performance of the device in comparison to a commercial device (Xitron Hydra 4200, Xitron Technologies) for that frequency range. An important item in this thesis has been the design of a multi-frequency current source able to perform measurements using capacitive electrodes

Hardware design of a portable medical device to measure the quadriceps muscle group after a total knee arthroplasty by EMG, LBIA and clinical score methods

El propòsit d'aquest projecte és el disseny del hardware d'un dispositiu mèdic portàtil per a mesurar senyals d'electromiografia (EMG) i bioimpedància localitzada (LBIA), que s'utilitzarà per avaluar la progressió de dues pròtesis de genoll (Medial-Pivot i Ultra- Congruent) en pacients operats d'una artroplàstia total de genoll per a l'hospital Germans Trias i Pujol de Badalona. Per això, s'ha realitzat un estudi complet sobre els senyals d'EMG i LBIA, per tal de definir les característiques necessàries de l'equip mèdic i poder optimitzar el disseny electrònic. Per l'adquisició de senyals EMG, s'ha dissenyat i simulat un sistema compost per diferents fases, que treballen independentment per adquirir, amplificar, filtrar i adaptar el senyal EMG pel seu futur processament digital. D'altra banda, per obtenir valors de la bioimpedància localitzada dels diferents músculs que conformen el quàdriceps, s'ha dissenyat un sistema compost per dos grans blocs; el primer bloc és l'etapa d'injecció, on es genera i s'injecta un senyal feble de corrent altern a la zona a mesurar, mentre que el segon bloc, és l'etapa d'adquisició de senyals. Aquest últim s'encarrega d'adquirir la diferència de voltatge produïda per la injecció de corrent al múscul (anteriorment mencionat) per després calcular la bioimpedància a partir de la llei d'ohm. Tots els senyals són digitalitzats mitjançant el microcontrolador STM32F407VG, que s'encarregarà de processar i aconseguir les dades claus per determinar quina de les deus pròtesis desenvolupa una millor funció mecànica i una millor adaptació biològica. És important remarcar que tot el disseny, sigui per a EMG o LBIA s'ha dut a terme de manera discreta sense fer servir Front-Ends comercials o integrats complexos més que l'amplificador d'instrumentació o ADC. En addició, el present treball inclou una primera estimació dels costos de producció i fabricació per a una sola unitat, càlculs de consums i funcionament (sorolls, CMRR del sistema i amplada de banda) i una simulació completa d'EMG i LBIA per observar com funciona i es du a terme cada etapa del circuit. Finalment, en tractar-se d'un equip mèdic, també s'ha revisat la normativa aplicable i se n'ha analitzat l'impacte ambiental, s'ha proposat i definit diferents punts per a futurs treballs, com podria ser la validació i testatge de l'equip, càlculs més aproximats de consums i perfilar la bill of materials (BOM) per a grans demandes de components.The purpose of this project is the hardware design of a portable medical device to measure electromyography (EMG) and localized bioimpedance (LBIA) signals, which will be used to evaluate the adaptability and progression of two knee prostheses (medial-pivot and ultra-congruent) in patients undergoing total knee arthroplasty at the Germans Trias i Pujol Hospital in Badalona. For this, the present work undercovers the relevant properties of the EMG and LBIA signals in order to define the characteristics of the medical equipment and thus optimize its electronic design. For the EMG measurements, a system made up of different stages has been designed and simulated. These phases work independently to acquire, amplify, filter, and adapt the EMG signal for its further digital processing. On the other hand, to obtain the bioimpedance values of different quadriceps muscles, a system composed of two large blocks has been designed; the first is the injection block, where a weak alternating current signal is generated and injected into the area to be measured, while the second block is the signal acquisition stage. The purpose of the latter is to acquire the voltage difference produced by the injection of current (mentioned above) and then obtain the bioimpedance from Ohm's law. All the signals are digitized from the STM32F407VG microcontroller, which will be in charge of processing and obtaining the key data to determine which of the two prostheses performs a better mechanical function and biological adaptation. It is important to note that the entire design, whether for EMG or LBIA, has been developed discreetly without using commercial Front-Ends or complex ICs other than the instrumentation amplifier or ADC. In addition, the thesis includes a first estimation of the production and manufacturing costs for a single unit, calculations of consumption and work operation (noise, CMRR of the system and bandwidth) and a complete simulation of EMG and LBIA to observe how it works on each stage for both circuits. Finally, as it is a medical device, the applicable regulations have also been reviewed and its environmental impact has been analysed. Additionally, different points have been proposed and defined for future work, such as the construction of the PCB and its respective validation, improving both the consumption calculations and the list of materials (BOM) for large component demands.El propósito de este proyecto es el diseño del Hardware de un dispositivo médico portátil para mediciones de electromiografía (EMG) y bioimpedancia localizada (LBIA), que se utilizará para estudiar la evolución de la adaptabilidad y funcionamiento de dos prótesis de rodilla (medial-pívot y ultracongruente) en pacientes operados de artroplastia total de rodilla en el Hospital Germans Trias i Pujol de Badalona. Para ello, se ha realizado un estudio exhaustivo sobre las propiedades de las señales de EMG y LBIA con la finalidad de definir las características del equipo médico y de esta forma, optimizar el diseño electrónico del mismo. Para la lectura de mediciones EMG, se ha diseñado y simulado un sistema constituido por distintas etapas, que trabajan independientemente para adquirir, amplificar, filtrar, y adaptarla señal EMG para su posterior procesado digital. Por otro lado, para obtener los valores de bioimpedancia de distintos músculos del cuádriceps, se ha diseñado un sistema compuesto por dos grandes bloques; el primero es el bloque de inyección, donde se genera y se inyecta una señal débil de corriente alterna en la zona a medir, mientras que el segundo bloque es la etapa de adquisición de señales. Esta última tiene como finalidad adquirir la diferencia de voltaje producido por la inyección de corriente (anteriormente mencionada) para después obtener la bioimpedancia a partir de la ley de ohm. Todas las señales son digitalizadas a partir del microcontrolador STM32F407VG, que se encargará de procesar y obtener los datos claves para determinar cuál de las dos prótesis desempeña una mejor función mecánica y adaptación biológica. Es importante remarcar que todo el diseño, ya sea para EMG o LBIA, se ha desarrollado de manera discreta sin usar Front-Ends comerciales o integrados complejos más que el amplificador de instrumentación o ADC. En adición, la tesis incluye una primera estimación de los costes de producción y fabricación para una sola unidad, cálculos de consumos y funcionamiento (ruidos, CMRR del sistema y ancho de banda) y una simulación completa de EMG y LBIA para observar cómo funciona y se desarrolla cada etapa de los distintos circuitos. Finalmente, al tratarse de un equipo médico, también se ha revisado la normativa aplicable y se ha analizado el impacto ambiental del mismo. Por último, se han propuesto y definido distintos puntos para futuros trabajos, como es la construcción de la PCB y su respectiva validación, realizar cálculos más aproximados de consumos y perfilar la lista de materiales (BOM) para grandes demandas de componentes

WEARABLE MULTI-SENSOR SYSTEM FOR TELEMEDICINE APPLICATIONS

In this paper, we describe a technical design of wearable multi-sensor systems for physiological data measurement and wide medical applications, significantly impacted in telehealth. The monitors are composed of three analog front-end (AFE) devices, which assist with interfacing digital electronics to the noise-, time-sensitive physiological sensors for measuring ECG (heart-rate monitor), RR (respiration-rate monitor), SRL (skin resistivity monitor). These three types of sensors can be used separately or together and allow to determine a number of parameters for the assessment of mental and physical condition. The system is designed based on requirements for demanding environments even outside the realm of medical applications, and in accordance with Health and Safety at Work directives (89/391/CE and Seveso-II 96/82/EC) for occupational hygiene, medical, rehabilitation, sports and fitness applications