Dual effect of Thymosin α 1 on human monocyte-derived dendritic cellin vitrostimulated with viral and bacterial toll-like receptor agonists

OBJECTIVES: Thymosin α 1 (Tα1) recently gained interest as immune adjuvant for vaccines because of its ability to modulate the T-cell/dendritic cell (DC) axis and to improve antibody production. The objective of this study was to determine whether Tα1 would address in vitro the response of human primary monocyte-derived DC, crucial regulators of vaccine-induced immunity, upon exposure to different toll-like receptor (TLR) agonists or infection with viruses or bacteria. METHODS: DC maturation and production of pro-inflammatory cytokines were analyzed. RESULTS: Our data revealed a dual effect of Tα1 on DC biology upon viral or bacterial stimulation. Interestingly, Tα1 enhanced human leukocyte antigen (HLA)-I and II surface expression and secretion of IL-6, TNF-α and IL-8 when DCs were treated with viral TLR3 and TLR7/8 agonists. Similarly, in pandemic H1N1 influenza A-infected DCs, Tα1 raised the expression of maturation markers and type I and III Interferon (IFN). In contrast, following bacterial TLR2 and 4 stimulation, as well as upon Bacillus Calmette-Guerin infection, the presence of Tα1 in DC cultures drastically lowered the analyzed cellular parameters. CONCLUSION: The knowledge that Tα1 pleiotropic effect might ameliorate anti-viral immune responses and, at the same time, dampen inflammation caused by bacterial infections could lay the groundwork for a more appropriate therapeutic application of this molecule

An agent-based DDM for high level architecture

The Data Distribution Management (DDM) service is one of the six services provided in the Runtime Infrastructure (RTI) of High Level Architecture (HLA). Its purpose is to perform data filtering and reduce irrelevant data communicated between federates. The two DDM schemes proposed for RTI, region based and grid based DDM, are oriented to send as little irrelevant data to subscribers as possible, but only manage to filter part of this information and some irrelevant data is still being communicated. Previously (G. Tan et al., 2000), we employed intelligent agents to perform data filtering in HLA, implemented an agent based DDM in RTI (ARTI) and compared it with the other two filtering mechanisms. The paper reports on additional experiments, results and analysis using two scenarios: the AWACS sensing aircraft simulation and the air traffic control simulation scenario. Experimental results show that compared with other mechanisms, the agent based approach communicates only relevant data and minimizes network communication, and is also comparable in terms of time efficiency. Some guidelines on when the agent based scheme can be used are also give

HLA high performance and real-time simulation studies with CERTI

Our work takes place in the context of the HLA standard and its application in real-time systems context. Indeed, current HLA standard is inadequate for taking into consideration the different constraints involved in real-time computer systems. Many works have been invested in order to provide real-time capabilities to Run Time Infrastructures (RTI). This paper describes our approach focusing on achieving hard real-time properties for HLA federations through a complete state of the art on the related domain. Our paper also proposes a global bottom up approach from basic hardware and software basic requirements to experimental tests for validation of distributed real-time simulation with CERTI

A Web Services Component Discovery and Deployment Architecture for Simulation Model Reuse

CSPs are widely used in industry, although have yet to operate across organizational boundaries. Reuse across organizations is restricted by the same semantic issues that restrict the inter-organization use of web services. The current representations of web components are predominantly syntactic in nature lacking the fundamental semantic underpinning required to support discovery on the emerging semantic web. Semantic models, in the form of ontology, utilized by web service discovery and deployment architecture provide one approach to support simulation model reuse. Semantic interoperation is achieved through the use of simulation component ontology to identify required components at varying levels of granularity (including both abstract and specialized components). Selected simulation components are loaded into a CSP, modified according to the requirements of the new model and executed. The paper presents the development carried out within CSPI-PDG and Fluidity Group at Brunel University, of an ontology, connector software and web service discovery architecture. The ontology is extracted from simulation scenarios involving airport, restaurant and kitchen service suppliers. The ontology engineering framework and discovery architecture provide a novel approach to inter-organization simulation, adopting a less intrusive interface between participants. Although specific to CSPs the work has wider implications for the simulation community

Semantic web services for simulation component reuse and interoperability: An ontology approach

Commercial-off-the-shelf (COTS) Simulation Packages (CSPs) are widely used in industry primarily due to economic factors associated with developing proprietary software platforms. Regardless of their widespread use, CSPs have yet to operate across organizational boundaries. The limited reuse and interoperability of CSPs are affected by the same semantic issues that restrict the inter-organizational use of software components and web services. The current representations of Web components are predominantly syntactic in nature lacking the fundamental semantic underpinning required to support discovery on the emerging Semantic Web. The authors present new research that partially alleviates the problem of limited semantic reuse and interoperability of simulation components in CSPs. Semantic models, in the form of ontologies, utilized by the authors’ Web service discovery and deployment architecture provide one approach to support simulation model reuse. Semantic interoperation is achieved through a simulation component ontology that is used to identify required components at varying levels of granularity (i.e. including both abstract and specialized components). Selected simulation components are loaded into a CSP, modified according to the requirements of the new model and executed. The research presented here is based on the development of an ontology, connector software, and a Web service discovery architecture. The ontology is extracted from simulation scenarios involving airport, restaurant and kitchen service suppliers. The ontology engineering framework and discovery architecture provide a novel approach to inter-organizational simulation, by adopting a less intrusive interface between participants Although specific to CSPs this work has wider implications for the simulation community. The reason being that the community as a whole stands to benefit through from an increased awareness of the state-of-the-art in Software Engineering (for example, ontology-supported component discovery and reuse, and service-oriented computing), and it is expected that this will eventually lead to the development of a unique Software Engineering-inspired methodology to build simulations in future

Developing interest management techniques in distributed interactive simulation using Java

Bandwidth consumption in distributed real time simulation, or networked real time simulation, is a major problem as the number of participants and the sophistication of joint simulation exercises grow in size. The paper briefly reviews distributed real time simulation and bandwidth reduction techniques and introduces the Generic Runtime Infrastructure for Distributed Simulation (GRIDS) as a research architecture for studying such problems. GRIDS uses Java abstract classes to promote distributed services called thin agents, a novel approach to implementing distributed simulation services, such as user defined bandwidth reduction mechanisms, and to distributing the executable code across the simulation. Thin agents offer the advantages of traditional agents without the overhead imposed by mobility or continuous state, which are unnecessary in this context. We present our implementation and some predicted results from message reduction studies using thin agent

Assessing and augmenting SCADA cyber security: a survey of techniques

SCADA systems monitor and control critical infrastructures of national importance such as power generation and distribution, water supply, transportation networks, and manufacturing facilities. The pervasiveness, miniaturisations and declining costs of internet connectivity have transformed these systems from strictly isolated to highly interconnected networks. The connectivity provides immense benefits such as reliability, scalability and remote connectivity, but at the same time exposes an otherwise isolated and secure system, to global cyber security threats. This inevitable transformation to highly connected systems thus necessitates effective security safeguards to be in place as any compromise or downtime of SCADA systems can have severe economic, safety and security ramifications. One way to ensure vital asset protection is to adopt a viewpoint similar to an attacker to determine weaknesses and loopholes in defences. Such mind sets help to identify and fix potential breaches before their exploitation. This paper surveys tools and techniques to uncover SCADA system vulnerabilities. A comprehensive review of the selected approaches is provided along with their applicability