Fungal Pathogens Infecting Soybean Aphid and Aphids on Other Crops Grown in Soybean Production Areas of Michigan

Seasonal prevalence of fungal pathogens infecting soybean aphid, Aphis glycines Matsumura (Hemiptera: Aphididae), was assessed from 2004 to 2006 in two Michigan soybean production areas. In 2005 and 2006 field-collected soybean aphids were incubated, and fungal infection was detected at both sites early in August 2005 during soybean pod development and high soybean aphid densities. Significantly higher proportions of winged aphid morphs were infected (20 and 90% infection at the two sites) than wingless aphid morphs (1 and 3% infection). All cases of mycosis examined involved one pathogen species, Pandora neoaphidis (Remaudiére & Hennebert) Humber (Entomophthorales: Entomophthoraceae). In 2004 and 2005, we surveyed for pathogens of the soy- bean aphid in soybean as well as pathogens in other aphid species feeding on other crop plants (alfalfa, clover, corn, and wheat) by inspecting for sporulating aphid cadavers every 2 to 3 wk during the soybean growing season. Aphid ca- davers were most abundant in alfalfa, especially in August; were less common in clover, corn, and soybean; and were not found in wheat. Pandora neoaphidis was associated with cadavers of Acyrthosiphon pisum (Harris) (Hemiptera: Aphididae) in alfalfa and clover during the same period when soybean aphid infection was detected. Overall, mortality of soybean aphid and other aphid species due to fungal infection was confirmed in Michigan. The results also implicate infected winged soybean aphid morphs as potential agents for fungal dispersal, and A. pisum in alfalfa and clover as a source of fungal propagules for soybean aphid

\u3ci\u3eHippodamia Variegata\u3c/i\u3e (Goeze) (Coleoptera: Coccinellidae) Detected in Michigan Soybean Fields

Since its initial detection near Montreal, Canada in 1984, the variegated lady beetle Hippodamia variegata (Goeze) (Coleoptera:Coccinellidae) has spread throughout the northeastern United States. In 2005, this immigrant Old World species was detected in Michigan for the first time. Twenty-nine adults were found in soybean fields in 4 counties: Ingham, Gratiot, Kalamazoo, and Saginaw.The first individuals were found in Gratiot County on 22 June 2005; we continued to detect individuals until 18 Aug 2005 (2 individuals collected in Saginaw Co.) when sampling ended. Prior to this study, H. variegata had not been known to prey on the soybean aphid, Aphis glycines Matsumura (Homoptera: Aphididae). The establishment of soybean aphid throughout the north-central U.S. may aid the spread of H. variegata throughout the region

Overexpression of Nictaba-like lectin genes from glycine max confers tolerance towards Pseudomonas syringae infection, aphid infestation and salt stress in transgenic Arabidopsis plants

Plants have evolved a sophisticated immune system that allows them to recognize invading pathogens by specialized receptors. Carbohydrate-binding proteins or lectins are part of this immune system and especially the lectins that reside in the nucleocytoplasmic compartment are known to be implicated in biotic and abiotic stress responses. The class of Nictaba-like lectins (NLL) groups all proteins with homology to the tobacco (Nicotiana tabacum) lectin, known as a stress-inducible lectin. Here we focus on two Nictaba homologs from soybean (Glycine max), referred to as GmNLL1 and GmNLL2. Confocal laser scanning microscopy of fusion constructs with the green fluorescent protein either transiently expressed in Nicotiana benthamiana leaves or stably transformed in tobacco BY-2 suspension cells revealed a nucleocytoplasmic localization for the GmNLLs under study. RT-qPCR analysis of the transcript levels for the Nictaba-like lectins in soybean demonstrated that the genes are expressed in several tissues throughout the development of the plant. Furthermore, it was shown that salt treatment, Phytophthora sojae infection and Aphis glycines infestation trigger the expression of particular NLL genes. Stress experiments with Arabidopsis lines overexpressing the NLLs from soybean yielded an enhanced tolerance of the plant towards bacterial infection (Pseudomonas syringae), insect infestation (Myzus persicae) and salinity. Our data showed a better performance of the transgenic lines compared to wild type plants, indicating that the NLLs from soybean are implicated in the stress response. These data can help to further elucidate the physiological importance of the Nictaba-like lectins from soybean, which can ultimately lead to the design of crop plants with a better tolerance to changing environmental conditions

Estimation de l'impact des parasitoïdes sur les populations de pucerons en champ

À partir d'un modèle existant de dynamique de populations de pucerons, nous avons développé une méthode permettant de quantifier la contribution de parasitoïdes à la réduction du maximum de densité de pucerons. La méthode a été validée, sur deux ans en champ de soya, en utilisant le modèle biologique composé du puceron du soya (Aphis glycines Matsumura) et de Aphelinus certus Jasnosh, son parasitoïde le plus abondant au nord-est de l'Amérique du Nord. La méthode a estimé que les densités naturelles de A. certus n'avait réduit les pics de densités de pucerons que de 1-6%. La cause de cette faible régulation est associée à un établissement tardif des populations de A. certus en champ de soya, mais les mécanismes sous-jacents restent inconnus. À cet effet, les proportions d'hyperparasitisme sur A. certus, avant le pic de densité de pucerons, étaient trop faible pour que l'hyperparasitisme puisse en être tenu responsable. Concernant la dynamique des populations de pucerons, nous avons proposé une re-paramétrisation du modèle mentionné précédemment afin de faciliter l'interprétation de ses paramètres lorsque la colonisation des champs par les pucerons n'était pas simultanée. Cette stratégie nous a permis d'établir que l'occurrence du pic de densité de puceron du soya est facilement prédictible de sa date de colonisation en champ. Nous recommandons l'utilisation de modèles afin de prédire les pics de densité de pucerons et d'utiliser la méthode d'estimation de l'impact afin d'incorporer des stratégies de relâchers augmentatifs de parasitoïdes aux programmes de lutte existants contre les pucerons.Building upon an existing aphid population dynamics model, we develop a method to quantify the contribution of parasitoids in reducing the maximum aphid density. We validated the method, over a two years study in soybean fields, using the biological model made of the soybean aphid (Aphis glycines Matsumura) and Aphelinus certus Jasnosh, its most abundant parasitoid in north-eastern North America. The method estimated that natural populations of A. certus reduced peak soybean aphid densities by only 1-6%. The cause of this low regulation is associated to the late establishment of A. certus population in soybean field, although the underlying mechanisms remains unknown. Proportion hyperparasitism on A. certus, before peak soybean aphid densities, were too low for hyperparasitism to be accounted for the poor efficacy of A. certus in regulating the soybean aphid. Regarding aphid population dynamics, we proposed a re-parameterisation of the aforementioned model to facilitate the interpretation of its parameters when field colonization by aphids is not simultaneous. This allowed us to identify a high predictability in peak aphid densities from colonization time for the soybean aphid. We recommend using models to forecast peak aphid densities and to use the impact assessment method to incorporate augmentative parasitoid release strategies into aphid management programmes

Study on Space Dynamics of a Natural Population of Aphis glycines Matsumura

The spatial distribution of soybean aphids on plants is evidently regulated over time, that is, soybean aphids move from the top parts of plants to lower parts in early period and from lower parts of plants to top parts in late period. The farm species group of soybean aphids shows aggregate distribution in its present process according to an analysis of the aggregate index. But the degree of aggregation of soybean aphids varies in different periods and shows nearly random distribution in its most damaging period.Originating text in Chinese.Citation: Shi, Shusen, Yi, Boren, Li, Dianshen, Yu, Yanjie. (1994). Study on Space Dynamics of a Natural Population of Aphis glycines Matsumura. Journal of Jilin Agricultural University, 16(12), 75-79

Population dynamics of soybean aphid Aphis glycines and its natural enemies in fields

Systematic survey and analysis in the fields showed that the peak damage period of Aphis glycines was the blooming stage of soybean. The density of soybean aphid reached 18815 per 100 plants and the dwarf rate of soybean plants was 20%. There were 15 species of main natural enemies. The density of natural enemies was 431 per 100 plants during peak period, the dominant natural enemies had 5 species, Propyloea japonica, Sphoerophoria sp., Erigonidium gramincola, Diaeretiella rapae, and Scymnus hoffmanni. The population dynamic curve of natural enemies resembled to that of Aphis glycines, but was delayed by 5-7 d.Originating text in Chinese.Citation: Han, Xincai. (1997). Population dynamics of soybean aphid Aphis glycines and its natural enemies in fields. Hubei Agricultural Sciences, 2, 22-39

Movement Patterns of Carabid Beetles Between Heterogenous Crop and Noncrop Habitats

Habitats adjacent to crop fields can increase natural enemy populations by providing additional food, shelter and overwintering sites. While many studies have focused on the role of non-crop borders for supporting natural enemies, here we investigate the influence of adjacent crop habitats as well. We monitored the movement of carabid beetles (Coleoptera: Carabidae) between wheat fields and adjacent crop and non-crop habitats using bi-directional pitfall traps. We found greater movement of carabids from corn into wheat fields than from forest and soybean, with intermediate levels of movement from roadside vegetation. Additionally, significantly more carabids were captured moving into corn from wheat than into any other habitat. We also found that carabid community assemblages at habitat borders were different from those in the interior of wheat fields. Our findings suggest that agricultural ecosystems composed of a variety of both non- crop and crop habitats are necessary to maintain carabid abundance and diversity

Abundance of Coccinellids and Their Potential Prey in Field-Crop and Grass Habitats in Eastern South Dakota

A rich fauna of coccinellids occurs in eastern South Dakota, but the abundance of some species has declined in association with the establishment of an exotic lady beetle, Coccinella septempunctata (L.) (Coleoptera: Coccinellidae), in the mid-1980s. In this study, coccinellids were sampled within field-crop and grass habitats in eastern South Dakota from 1990 to 1995 to survey for various coccinellid species and to determine any effects of habitat management on abundance. Field crops (maize, wheat-alfalfa intercrop, and alfalfa) were subjected to high, intermediate, or low crop-management intensity (CMI), and grass habitats were managed for stands of warm season, cool season, or mixed species. A total of 1,306 adult and 155 larval coccinellids were collected. Four native species (Coleomegilla maculate (DeGeer), Hippodamia convergens Guerin Meneville, Hippodamia parenthesis (Say), andHippodamia tredecimpunctata tibialis (Say)) and C. septempunctata comprised over 96 percent of all coccinellids collected. Of declining species, four Coccinella transversoguttata richardsoni Brown were collected from alfalfa, but Coccinella novemnotata Herbst and Adalia bipunctata (L.) were not found during the study. Coccinellid abundance was seldom affected by CMI. Coccinellids were more abundant in wheat-alfalfa in 1995 under high than low CMI. Coccinellid abundance in maize and alfalfa did not differ with CMI. A prey species, Empoasca fabae (Harris) (Heteroptera: Auchenorryncha: Cicadellidae), was more abundant in alfalfa in 1995 under high and intermediate than under low CMI. Coccinellid abundance was not correlated with that of E. fabae in 1995, and showed inconsistent association with E. fabae during the study. In grass, adult coccinellids (total across species), adult H. tredecimpunctata tibialis, and aphids were more abundant in warm- season grasses than in cool-season or mixed grass stands in one of three years. Our results provide further evidence that C. septempunctata has become relatively abundant in eastern South Dakota, whereas C. transversoguttata richardsoni, C. novemnotata, and A. bipunctata have become rare or absent