5,947 research outputs found

    Non-invasive and non-intrusive diagnostic techniques for gas-solid fluidized beds – A review

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    Gas-solid fluidized-bed systems offer great advantages in terms of chemical reaction efficiency and temperature control where other chemical reactor designs fall short. For this reason, they have been widely employed in a range of industrial application where these properties are essential. Nonetheless, the knowledge of such systems and the corresponding design choices, in most cases, rely on a heuristic expertise gained over the years rather than on a deep physical understanding of the phenomena taking place in fluidized beds. This is a huge limiting factor when it comes to the design, the scale-up and the optimization of such complex units. Fortunately, a wide array of diagnostic techniques has enabled researchers to strive in this direction, and, among these, non-invasive and non-intrusive diagnostic techniques stand out thanks to their innate feature of not affecting the flow field, while also avoiding direct contact with the medium under study. This work offers an overview of the non-invasive and non-intrusive diagnostic techniques most commonly applied to fluidized-bed systems, highlighting their capabilities in terms of the quantities they can measure, as well as advantages and limitations of each of them. The latest developments and the likely future trends are also presented. Neither of these methodologies represents a best option on all fronts. The goal of this work is rather to highlight what each technique has to offer and what application are they better suited for

    Efficient low rank approximations for parabolic control problems with unknown heat source

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    An inverse problem of finding an unknown heat source for a class of linear parabolic equations is considered. Such problems can typically be converted to a direct problem with non-local conditions in time instead of an initial value problem. Standard ways of solving these non-local problems include direct temporal and spatial discretization as well as the shooting method, which may be computationally expensive in higher dimensions. In the present article, we present approaches based on low-rank approximation via Arnoldi algorithm to bypass the computational limitations of the mentioned classical methods. Regardless of the dimension of the problem, we prove that the Arnoldi approach can be effectively used to turn the inverse problem into a simple initial value problem at the cost of only computing one-dimensional matrix functions while still retaining the same accuracy as the classical approaches. Numerical results in dimensions d=1,2,3 are provided to validate the theoretical findings and to demonstrate the efficiency of the method for growing dimensions

    Synthesis of multifunctional glyco-pseudodendrimers and glyco-dendrimers and their investigation as anti-Alzheimer agents

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    As the world population is aging, the cases of Alzheimer’s Disease (AD) are increasing. AD is a disorder of the brain which is characterized by the aggregation of amyloid beta (Aβ) plaques. This leads to the death of numerous brain cells thus affecting the cognitive and motor functions of the individual. Till date, no cure for the disease is available. Aβ are peptides with 40/42 amino acid residues but, their exact mechanism(s) of action in AD is under debate. Having different amino acid residues makes them susceptible to form hydrogen bonds. Dendrimers with sugar units are often referred to as glycopolymers and have been shown to have potential anti-amyloidogenic activity. However, they also have drawbacks, the synthesis involves multiple tedious steps, and dendrimers themselves offer only a limited number of functional units. Pseudodendrimers are another class of branched polymers based on hyperbranched polymers. Unlike the dendrimers, they are easy to synthesize with a dense shell of functional units on the surface. One of the main goals in this dissertation is the synthesis and characterization of pseudodendrimers and dendrimers based on 2,2-bis(hydroxymethyl)-propionic acid (bis-MPA), an aliphatic polyester scaffold, as it offers biocompatibility and easy degradability. Furthermore, they are decorated with mannose units on the surface using a ‘click’ reaction forming glyco-pseudodendrimers and glyco-dendrimers. A detailed characterization of their structures and physical properties was undertaken using techniques such as size exclusion chromatography, asymmetric flow field flow fractionation (AF4), and dynamic light scattering. The second main focus of this work has been to investigate the interaction of synthesized glyco-pseudodendrimers and glyco-dendrimers with Aβ 40 peptides. For this task, five different concentrations of the synthesized glycopolymers were tested with Aβ 40 using the Thioflavin T assay. The results of the synthesized polymers which produced the best results of showing maximum anti-aggregation behavior against Aβ 40 were confirmed with circular dichroism spectroscopy. AF4 was also used to investigate Aβ 40-glycopolymer aggregates, which has never been done before and constitutes the highlight of this dissertation. Atomic force microscopy was used to image Aβ 40-glycopseudodenrimer aggregates. A basic but important step in the development of drug delivery platforms is to evaluate the toxicity of the drugs synthesized. In this work, preliminary studies of the cytotoxicity of glyco-pseudodendrimers were performed in two different cell lines. Thus, this study comprises a preliminary investigation of the anti-amyloidogenic activity of glyco-pseudodendrimers synthesized on an aliphatic polyester backbone.:Abstract List of Tables List of Figures Abbreviations 1 Introduction 1.1 Objectives of the work 1.2 Thesis overview 2 Fundamentals and Literature 2.1 Alzheimer’s Disease and its impact 2.1.1 Neurological diagnosis of AD 2.1.2 Histopathology of AD 2.1.3 Amyloid precursor protein (APP) and its role in AD 2.2. Amyloid Beta (Aβ) peptide 2.2.1 Aβ peptide 2.2.2. Location and function 2.2.3 Amyloid hypothesis 2.2.4 The mechanism of Aβ aggregation 2.2.5 Amyloid fibrils 2.2.6 Toxicity of Aβ 2.3 Research methods to study Aβ aggregates 2.3.1 Models to study the mode of action of aggregates 2.3.2 Endogenous Aβ aggregates and synthetic aggregates 2.3.3 Strategies to alter aggregation of amyloids 2.4 Treatment and therapeutics 2.4.1 Current therapeutics 2.4.2 Current therapeutic research 2.4.2.1 Reduction of Aβ production 2.4.2.2 Reduction of Aβ plaque accumulation 2.4.2.2.1 Anti-amyloid aggregation agents 2.4.2.2.2 Metals 2.4.2.2.3 Immunotherapy 2.4.2.2.4 Dendrimers as potential anti-amyloidogenic agent 2.6 Dendrimers 2.6.1 Definition 2.6.2 Structure Table of Contents 2.6.3 Synthesis 2.6.4 Properties 2.7 Pseudodendrimers - a sub-class of hyperbranched polymer 2.7.1 Definition 2.7.2 Structure 2.7.3 Synthesis 3 Analytical Techniques 3.1 Size Exclusion Chromatography Coupled to Light Scattering (SEC-MALS) 3.2 Asymmetric Flow Field Flow Fractionation (AF4) 3.3 Dynamic Light Scattering 3.4 Molecular Dynamics Simulation 3.5 Nuclear Magnetic Resonance Spectroscopy 3.6 Thioflavin T fluorescence 3.6.1 Kinetic analysis 3.7 Circular Dichroism Spectroscopy 3.8 Atomic Force Microscopy 3.9 Cytotoxic assay 3.9.1 MTT assay 3.9.2 Determining the level of reactive oxygen species 3.9.3 Changes in mitochondrial transmembrane potential 3.9.4 Flow cytometric detection of phosphatidyl serine exposure 4 Experimental Details and Methodology 4.1 Details of chemicals/components used 4.1.1 Other materials 4.1.2 Peptide preparation 4.1.3 Buffer preparation 4.1.4 Fibril growth conditions 4.2 Synthesis and characterization of polymers 4.2.1 Synthesis and characterization of pseudodendrimers and dendrimers 4.2.1.1 Synthesis of hyperbranched polymer (1) 4.2.1.2 Synthesis of protected monomer 4.2.1.2.1 bis-MPA acetonide (2) 4.2.1.2.2 bis-MPA-acetonide anhydride (3) 4.2.1.3 Synthesis of protected pseudodendrimers (4, 6 and 8) and protected dendrimers (10, 12, and 14) 4.2.1.4 Deprotection of pseudodendrimers (5,7, and 9) and dendrimers (11,13 and 15) 4.2.2 Synthesis of glyco-pseudodendrimers and glyco-dendrimers 4.2.2.1 Pentynoic anhydride (16) 4.2.2.2 Synthesis of pentinate modified pseudodendrimers (17, 18 and 19) and dendrimers (20, 21 and 22) 4.2.2.3 3-Azido-1-propanol (23) 4.2.2.4 Mannose propyl azide tetraacetate (24) Table of Contents 4.2.2.5 Mannosepropylazide (25) 4.2.2.6 Glyco-pseudodendrimers (Gl-P) (26, 27 and 28) and glyco- dendrimers (Gl-D) (29, 30 and 31) 4.3 Analytical techniques and their general details 4.3.1 SEC-MALS - Instrumentation, software and analysis 4.3.2 AF4 - Instrumentation, software and analysis 4.3.2.1 Sample preparation 4.3.2.2 Method development for analysis of Gl-P and Gl-D 4.3.2.3 Method development for analysis of Aβ 40 and its interaction with Gl-P and Gl-D 4.3.3 Batch DLS - Instrumentation, software and analysis 4.3.3.1 Sample preparation 4.3.4 Theoretical calculations and molecular dynamics simulations 4.3.4.1 Ab-initio calculations 4.3.4.2 Modelling of the polymer structures 4.3.4.2.1 Pseudodendrimers 4.3.4.2.2 Dendrimers 4.3.4.2.3 Modification of the polymers with special end groups 4.3.4.2.4 Preparing of the THF solvent box 4.3.4.2.5 Solvation of the polymer structures 4.3.4.3 Molecular dynamics simulations 4.3.4.3.1 Evaluation of the simulation trajectories 4.4 Investigation of interaction of Gl-P and Gl-D with amyloid beta (Aβ 40) 4.4.1 ThT Assay - Instrumentation and software 4.4.1.1 Sample preparation 4.4.1.2 Kinetics based on ThT assay- software and data analysis 4.4.2 CD spectroscopy - Instrumentation and software 4.4.2.1 Sample preparation 4.4.3 AFM - Instrumentation and software 4.4.3.1 Substrate and sample preparation 4.4.3.2 Height determination and counting procedures 4.4.3.3 Topography and diameter 4.5 Cytotoxicity 4.5.1 Zeta potential 4.5.2 Cell culturing 4.5.3 Sample preparation 4.5.4 MTT assay 4.5.5 Changes in mitochondrial transmembrane potential (JC-1 method) 4.5.6 Flow cytometric detection of phosphatidyl serine exposure (Annexin V and PI method) 5 Results and Discussion 5.1 Synthesis and characterization of glyco-pseudodendrimers and glyco- dendrimers 5.1.1 Synthesis and characterization of hyperbranched polyester Table of Contents 5.1.2 Synthesis and characterization of pseudodendrimers P-G1-OH, P-G2-OH and P-G3-OH 5.1.3 Synthesis and characterization of dendrimers D-G4-OH, D-G5-OH and D-G6-OH 5.1.4 Synthesis and characterization of Gl-P and Gl-D 5.1.4.1 Molecular size determination of Gl-P and Gl-D using SEC 5.1.4.2 Particle size determination using batch DLS 5.1.4.3 Apparent densities 5.1.4.4 Molecular size determination of Gl-P and Gl-D using AF4 ..... 5.1.5 Molecular dynamics simulation 5.2 Investigation of interaction of Gl-P and Gl-D with amyloid beta (Aβ 40) ...... 5.2.1 ThT Assay 5.2.1.1 Kinetics based on ThT assay 5.2.2 CD spectroscopy 5.2.3 Time dependent AF4 5.3.2.1 Separation of Aβ 40 by AF4 5.3.2.2 Aβ 40 amyloid aggregation in the presence of Gl-P and Gl-D 5.2.4 AFM 5.2.4.1 Height 5.2.4.2 Topography and diameter 5.2.4.3 Length 5.2.4.4 Morphology 5.2.5 Cytotoxicity 5.2.5.1 MTT assay 5.2.5.2 Changes in mitochondrial transmembrane potential 5.2.5.3 Flow cytometric detection of phosphatidyl serine exposure 6 Conclusions and Outlook 7 Bibliography Appendix Acknowledgement

    Proceedings of SIRM 2023 - The 15th European Conference on Rotordynamics

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    It was our great honor and pleasure to host the SIRM Conference after 2003 and 2011 for the third time in Darmstadt. Rotordynamics covers a huge variety of different applications and challenges which are all in the scope of this conference. The conference was opened with a keynote lecture given by Rainer Nordmann, one of the three founders of SIRM “Schwingungen in rotierenden Maschinen”. In total 53 papers passed our strict review process and were presented. This impressively shows that rotordynamics is relevant as ever. These contributions cover a very wide spectrum of session topics: fluid bearings and seals; air foil bearings; magnetic bearings; rotor blade interaction; rotor fluid interactions; unbalance and balancing; vibrations in turbomachines; vibration control; instability; electrical machines; monitoring, identification and diagnosis; advanced numerical tools and nonlinearities as well as general rotordynamics. The international character of the conference has been significantly enhanced by the Scientific Board since the 14th SIRM resulting on one hand in an expanded Scientific Committee which meanwhile consists of 31 members from 13 different European countries and on the other hand in the new name “European Conference on Rotordynamics”. This new international profile has also been emphasized by participants of the 15th SIRM coming from 17 different countries out of three continents. We experienced a vital discussion and dialogue between industry and academia at the conference where roughly one third of the papers were presented by industry and two thirds by academia being an excellent basis to follow a bidirectional transfer what we call xchange at Technical University of Darmstadt. At this point we also want to give our special thanks to the eleven industry sponsors for their great support of the conference. On behalf of the Darmstadt Local Committee I welcome you to read the papers of the 15th SIRM giving you further insight into the topics and presentations

    Development, Implementation, and Optimization of a Modern, Subsonic/Supersonic Panel Method

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    In the early stages of aircraft design, engineers consider many different design concepts, examining the trade-offs between different component arrangements and sizes, thrust and power requirements, etc. Because so many different designs are considered, it is best in the early stages of design to use simulation tools that are fast; accuracy is secondary. A common simulation tool for early design and analysis is the panel method. Panel methods were first developed in the 1950s and 1960s with the advent of modern computers. Despite being reasonably accurate and very fast, their development was abandoned in the late 1980s in favor of more complex and accurate simulation methods. The panel methods developed in the 1980s are still in use by aircraft designers today because of their accuracy and speed. However, they are cumbersome to use and limited in applicability. The purpose of this work is to reexamine panel methods in a modern context. In particular, this work focuses on the application of panel methods to supersonic aircraft (a supersonic aircraft is one that flies faster than the speed of sound). Various aspects of the panel method, including the distributions of the unknown flow variables on the surface of the aircraft and efficiently solving for these unknowns, are discussed. Trade-offs between alternative formulations are examined and recommendations given. This work also serves to bring together, clarify, and condense much of the literature previously published regarding panel methods so as to assist future developers of panel methods

    Influence of viscoelasticity on the displacement of capillary entrapments

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    It is already evident that the viscoelastic nature of polymer solutions affects the displacement processes when such fluids are used as displacing fluids, for example in polymer flooding. However, a clear explanation of how exactly viscoelasticity plays a role at the microscale and enhances microscopic displacement efficiency in porous media is still lacking. In this dissertation, a possible mechanism of displacing capillary entrapment under the sole influence of elastic stresses is presented. A model system consisting of two serpentine channels connected in the middle by a capillary channel has been designed to represent a single pore entrapment. The serpentine channel is particularly important to mimic the tortuous nature of flow in porous media. Direct experimental observations in this model system show that the interface between the entrapped phase and the viscoelastic polymer solution used as the displacing phase becomes unstable above a certain shear rate, eventually leading to the complete removal of the entrapped phase. The results of Particle Image Velocimetry experiments of the polymer flow in the serpentine channel show that the origin of these interfacial fluctuations is the velocity fluctuations due to purely elastic instabilities and strong elastic secondary flow structure.Wenn Polymerlösungen als Verdrängungsmittel verwendet werden, z.B. beim ”Polymerfluten”, wurde bereits nachgewiesen, dass die Viskoelastizität von Polymerlösungen die Verdrängungsprozesse beeinflusst. Es gibt jedoch keine klare Erklärung dafür, welche Rolle die Viskoelastizität bei der Verdrängung auf der Mikrometerskala spielt und wie sie die mikroskopische Verdrängungseffizienz in porösen Medien verbessert. In dieser Dissertation wird ein möglicher Mechanismus für die Verdrängung von Kapillareinschlüssen unter dem ausschließlichen Einfluss von elastischen Spannungen in der verdrängenden Flüssigkeit vorgestellt. Das verwendete mikrofluidische Modellsystem, das aus zwei Serpentinen-Kanälen besteht, die in der Mitte durch einen Kanal verbunden sind, wurde so gestaltet, dass es einen einzelnen Poreneinschluss simuliert. Die Serpentinen-Kanäle sind besonders wichtig, um die gewundene Natur der Strömung in porösen Medien zu imitieren. Direkte experimentelle Beobachtungen in diesem Modellsystem zeigen, dass die Grenzfläche zwischen der eingeschlossenen Phase und der viskoelastischen Verdrängungsphase oberhalb einer kritischen Scherrate instabil wird, was schließlich zur vollständigen Entleerung der eingeschlossenen Phase führt. Die Analyse des geschwindigkeitsabhängigen Strömungsverhaltens der Polymerl ösung im Serpentinen-Kanal mittels μPIV zeigt, dass der Ursprung dieser Grenzfl ächenfluktuationen die Geschwindigkeitsschwankungen aufgrund von rein elastischen Instabilitäten und stark elastischen sekundären Strömungsstrukturen sind. Daraus lässt sich schließen, dass diese elastizitätsbedingten Instabilitäten die Verschiebung des kapillaren Einschlusses in den porösen Medien verursachen

    Luminescent Nanocrystals: Line broadening and formation mechanisms

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    Nanomaterials have become an increasingly important class of materials in the past decades due to their size-tunable optical, electronic, and magnetic properties. Nanomaterials are not only of great scientific interest, but their versatility has also resulted in a wide range of applica¬tions. This thesis focuses on two types of luminescent (light-emitting) nanomaterials, cadmium chalcogenide nanocrystals (NCs) and NaYF4 NCs doped with rare earth ions (lanthanides, e.g., erbium and ytterbium). Both the optical properties and nanocrystal growth mechanisms are investigated. Semiconductor NCs, especially CdSe nanoplatelets (NPLs), exhibit narrow emission bands in the visible part of the spectrum, a property needed for more efficient white light LEDs (w-LEDs) and vibrant displays. In these applications, the luminescent materials operate at elevated tem¬peratures, which affects the emission linewidth. Insight into this thermal broadening is important for application in w-LEDs but has so far not been investigated over a temperature range that is relevant for these applications. In this thesis, I report on the temperature-dependent spectral linewidth of cadmium chalcogenide NPLs and QDs. NaYF4 NCs doped with lanthanide ions are efficient upconversion materials that can convert two low-energy infrared photons to one high-energy visible photon. These materials can be used in deep-tissue imaging and to enhance the efficiency of solar cells. The formation mechanism of both NaYF4 NCs and CdSe NPLs is still debated. Control over the NC growth is essential to adjust the NC properties. In this thesis, I report on the mechanisms of their nucleation and growth, monitored using in situ absorption and x-ray scattering techniques

    Beam scanning by liquid-crystal biasing in a modified SIW structure

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    A fixed-frequency beam-scanning 1D antenna based on Liquid Crystals (LCs) is designed for application in 2D scanning with lateral alignment. The 2D array environment imposes full decoupling of adjacent 1D antennas, which often conflicts with the LC requirement of DC biasing: the proposed design accommodates both. The LC medium is placed inside a Substrate Integrated Waveguide (SIW) modified to work as a Groove Gap Waveguide, with radiating slots etched on the upper broad wall, that radiates as a Leaky-Wave Antenna (LWA). This allows effective application of the DC bias voltage needed for tuning the LCs. At the same time, the RF field remains laterally confined, enabling the possibility to lay several antennas in parallel and achieve 2D beam scanning. The design is validated by simulation employing the actual properties of a commercial LC medium
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