RECONSTITUTION OF ALLOPHYCOCYANIN FROM Mastigocladus laminosus WITH ISOLATED LINKER POLYPEPTIDE

The core linker polypeptide Lc 8.9 was isolated from Mastigocladus laminosus and purified on a preparative scale. A method for the reconstitution of allophycocyanin (AP)—linker complexes from isolated polypeptides was developed. The complex (αAP(βAP)3 Lc 8.9 was reconstituted and compared to (αAPβAP) and (αAPβAP)3 by sucrose density gradient ultracentrifugation, absorption, fluorescence emission and circular dichroism spectroscopy. Differences in the spectra of reconstituted and of directly isolated AP complexes are discussed

Optimization of the Ballistic Guide Design for the SNS FNPB 8.9 A Neutron Line

The optimization of the ballistic guide design for the SNS Fundamental Neutron Physics Beamline 8.9 A line is described. With a careful tuning of the shape of the curve for the tapered section and the width of the straight section, this optimization resulted in more than 75% increase in the neutron flux exiting the 33 m long guide over a straight m=3.5 guide with the same length.Comment: 21 pages, 13 figures; added a paragraph on existing ballistic guides to respond to referee comments; accepted for publication in Nuclear Inst. and Methods in Physics Research,

Patient-Reported Side Effects of Intradetrusor Botulinum Toxin Type A for Idiopathic Overactive Bladder Syndrome

Objective: The aim of the study was a prospective assessment of patient-reported side effects in an open-label study after intradetrusor botulinum toxin injections for idiopathic overactive bladder (OAB). Patients and Methods: Botulinum toxin A injection was performed in 56 patients with idiopathic OAB. Patients were followed up for 6 months concerning side effects and patients' satisfaction. Results: Different types of side effects were assessed such as dry mouth (19.6%), arm weakness (8.9%), eyelid weakness (8.9%), leg weakness (7.1%), torso weakness (5.4%), impaired vision (5.4%) and dysphagia (5.4%). In all cases, symptoms were mild and transient. Urological complications such as gross hematuria (17.9%), acute urinary retention (8.9%) and acute urinary tract infection (7.1%) were noticed. In all cases, acute urinary retention was transient and treated with temporary intermittent self-catheterization. There was no statistically significant correlation between dosage and observed side effects. Patients' satisfaction rate was high (71.4%). Conclusion: Intradetrusor injection of botulinum toxin was associated with a high rate of neurourological side effects. In general, side effects were transient, mild and did not require special treatment. Copyright (C) 2010 S. Karger AG, Base

Electrochemical behavior of Sn-Zn alloys with different grain structures in chloride-containing solutions

In the present research the electrochemical behavior of the Sn-Zn alloys (Sn-1 wt.%Zn, Sn-4 wt.%Zn and 8.9 wt.%Zn) in 3% NaCl solution is analyzed using potentiodynamic cyclic polarization measurements and Electrochemical Impedance Spectroscopy (EIS) technique. Specimens were longitudinally solidified with simultaneous heat extraction in two opposite directions. Working electrodes were constructed using longitudinal and cross sections of the specimens with both types of structure: columnar and equiaxed. Results obtained from the polarization curves indicated that the two types of grain structures of Sn-Zn alloys (Sn-1 wt.%Zn, Sn-4 wt.%Zn and Sn-8.9 wt.%Zn) corresponding to longitudinal section present a pseudo passive zone. In the case of specimens from cross sections of the samples, the columnar and equiaxed zones of Sn-8.9 wt.%Zn are the only ones that do not have this pseudo passive region. In addition, the interdendritic zone of alloys is susceptible to corrosion by dealloying because this phase is zinc-rich. This type of corrosion also occurs in the zinc rich lamellar structure present in the eutectic. The percentage of zinc in the alloy increases with increasing susceptibility to pitting corrosion. The EIS values obtained revealed that the susceptibility to corrosion increases with increasing zinc content in alloys, for both the columnar and equiaxed zones. In addition, the columnar zones of Sn-4 wt.%Zn and Sn-8.9 wt.%Zn specimens are more resistant to corrosion than the equiaxed grain specimens. However, the equiaxed zone of Sn-1 wt.%Zn alloy is less susceptible to corrosion than the columnar zone. After adjustment by equivalent circuits it is revealed that the equiaxed zone of Sn-8.9 wt.%Zn alloy has a second porous layer composed of corrosion products on the electrode surface.Fil: Mendez, Claudia Marcela. Universidad Nacional de Misiones. Facultad de Ciencias Exactas, Químicas y Naturales; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Nordeste. Instituto de Materiales de Misiones. Universidad Nacional de Misiones. Facultad de Ciencias Exactas Químicas y Naturales. Instituto de Materiales de Misiones; ArgentinaFil: Scheiber, Verónica L.. Provincia de Misiones. Comité de Desarrollo e Innovación Tecnológica. Centro de Desarrollo e Innovación Tecnológica; ArgentinaFil: Rozicki, Roberto S.. Universidad Nacional de Misiones. Facultad de Ciencias Exactas, Químicas y Naturales; ArgentinaFil: Kociubczyk, Alex Iván. Universidad Nacional de Misiones. Facultad de Ciencias Exactas, Químicas y Naturales; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Nordeste. Instituto de Materiales de Misiones. Universidad Nacional de Misiones. Facultad de Ciencias Exactas Químicas y Naturales. Instituto de Materiales de Misiones; ArgentinaFil: Ares, Alicia Esther. Universidad Nacional de Misiones. Facultad de Ciencias Exactas, Químicas y Naturales; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Nordeste. Instituto de Materiales de Misiones. Universidad Nacional de Misiones. Facultad de Ciencias Exactas Químicas y Naturales. Instituto de Materiales de Misiones; Argentin

8.9. Threat: Pollution

Unknown effectiveness (limited evidence) ● Mow shrubland to reduce impact of pollutants One randomized, replicated, controlled study in the UK found that mowing to reduce the impact of nitrogen deposition did not alter shoot length of common heather or the number of purple moor grass seedlings. One controlled study in the UK found that mowing a heathland affected by nitrogen pollution did not alter the cover or shoot length of heather compared to areas where prescribed burning was used. Asses..