Genetic Locus Required for Antigenic Maturation of \u3cem\u3eRhizobium etli\u3c/em\u3e CE3 Lipopolysaccharide

Rhizobium etli modifies lipopolysaccharide (LPS) structure in response to environmental signals, such as low pH and anthocyanins. These LPS modifications result in the loss of reactivity with certain monoclonal antibodies. The same antibodies fail to recognize previously isolated R. etli mutant strain CE367, even in the absence of such environmental cues. Chemical analysis of the LPS in strain CE367 demonstrated that it lacked the terminal sugar of the wild-type O antigen, 2,3,4-tri-O-methylfucose. A 3-kb stretch of DNA, designated as lpe3, restored wild-type antigenicity when transferred into CE367. From the sequence of this DNA, five open reading frames were postulated. Site-directed mutagenesis and complementation analysis suggested that the genes were organized in at least two transcriptional units, both of which were required for the production of LPS reactive with the diagnostic antibodies. Growth in anthocyanins or at low pH did not alter the specific expression of gusA from the transposon insertion of mutant CE367, nor did the presence of multiple copies of lpe3 situated behind a strong, constitutive promoter prevent epitope changes induced by these environmental cues. Mutations of the lpe genes did not prevent normal nodule development on Phaseolus vulgaris and had very little effect on the occupation of nodules in competition with the wild-type strain

Liquid phase epitaxy and optical investigation of KYb(WO4)2 thin layers

In recent years, Yb3+ has attracted much attention as an activating ion because of its small quantum defect for laser emission from 2F5/2 to 2F7/2 at ~1.03 µm [1], which provides high efficiency and reduced heat generation. Of high practical interest is the thin-disk laser concept [2], which possesses a tremendous advantage over rod lasers because of its axial-cooling approach and consequent weak thermal lensing and good beam quality.\ud A promising material for Yb3+ thin-disk lasers is KYb(WO4)2 (KYbW) [3]. It can be grown from high-temperature solutions [4]. Nevertheless, the growth of high-quality, single-crystalline layers with thickness in the range of the absorption length of ~13 µm at 981 nm has as yet not been reported. A suitable substrate material is KY(WO4)2 (KYW), but the relatively large differences in the thermal expansion coefficients between KYW and KYbW along the [100], [001], and especially [010] directions [5] favor low temperatures for the hetero-epitaxial growth.\ud For the first time, we demonstrate liquid phase epitaxy (LPE) of KYbW layers. The layers were grown at start temperatures as low as 520°C, which is favorable in order to decrease the thermal stresses due to the differences in the thermal expansion coefficients of substrate and layer. Moreover, the choice of [010]-oriented substrates bypasses the large difference in the thermal expansion coefficient along the [010] direction. KY1-xYbx(WO4)2 layers with varying x = 0.03-1.00 were grown by LPE. The chloride solvent consisted of the eutectic composition [6] 24.4 mol.% KCl, 30.4 mol.% NaCl, and 42.2 mol.% CsCl. The growth temperature spanned the range from 580 to 500°C and the cooling rate was 0.67-1.00 Kh-1. Crack-free, transparent KYbW layers were grown on (010) substrates.\ud Spectroscopic investigations have shown that the lifetime of ~250 µs measured in our LPE-grown KYbW layers is dominated by radiative decay and is very similar to that measured in top-seeded-solution-grown bulk samples [4]. Fast energy migration among the Yb3+ ions and energy transfer to small amounts of Tm3+ and Er3+ ions present in the YbCl3 reagent lead to visible upconversion luminescence in the layers under 981-nm excitation.\ud \ud [1] T.Y. Fan, IEEE J. Quantum Electron. 29, 1457 (1993).\ud [2] A. Giesen, H. Hügel, A. Voss, K. Wittig, U. Brauch, H. Opower, Appl. Phys. B 58, 365 (1994).\ud [3] P. Klopp, U. Griebner, V. Petrov, X. Mateos, M.A. Bursukova, M.C. Pujol, R. Solé, J. Gavaldà, M. Aguiló, F. Güell, J. Massons, T. Kirilov, F. Díaz, Appl. Phys. B 74, 185 (2002).\ud [4] M.C. Pujol, M.A. Bursukova, F. Güell, X. Mateos, R. Solé, J. Gavaldà, M. Aguiló, J. Massons, F. Díaz, P. Klopp, U. Griebner, V. Petrov, Phys. Rev. B 65, 165121 (2002).\ud [5] M.C. Pujol, X. Mateos, R. Solé, J. Massons, J. Gavaldà, F. Díaz, M. Aguiló, Mater. Sci. Forum 378-381, 710 (2001).\ud [6] D. Ehrentraut, M. Pollnau, S. Kück, Appl. Phys. B 75, 59 (2002)

The impact of alpha/Fe enhanced stellar evolutionary tracks on the ages of elliptical galaxies

We complement our study of alpha/Fe enhanced stellar population models of Lick absorption indices (Thomas et al. 2003) by comparing two sets of alpha/Fe enhanced models. In both models the impact on Lick indices due to alpha/Fe enhancement is accounted for through a modification of the stellar absorption line-strengths using the response functions of Tripicco & Bell (1995). One set of models, however, uses solar-scaled, the other alpha/Fe enhanced stellar evolutionary tracks. Since the alpha/Fe enhanced tracks are hotter than the solar-scaled ones (Salasnich et al. 2000), the correspondent stellar population models have slightly weaker metallic indices (i.e. Mgb, etc.) and stronger Balmer line indices (Hbeta) (Maraston et al 2003). Here we explore quantitatively the impact of this effect on the alpha/Fe ratios, metallicities and ages that are derived for elliptical galaxies. We find that the modest decrease of the metallic indices Mgb and balance each other, such that fully consistent alpha/Fe ratios are derived for stellar systems using alpha/Fe enhanced models with either solar-scaled or alpha/Fe enhanced stellar tracks. The decrease of the metallic indices and the increase of Hbeta conspire in a way that also consistent metallicities are obtained. The derived ages, instead, are significantly different. The inclusion of alpha/Fe enhanced stellar tracks leads to the derivation of ages as high as 30 Gyr for elliptical galaxies. For the same objects, ages not older than 15 Gyr are obtained, if alpha/Fe enhanced models using solar-scaled tracks are adopted. This may indicate that current stellar evolutionary models overestimate the bluing of stellar evolutionary tracks due to alpha/Fe enhanced chemical mixtures at super-solar metallicities.Comment: 5 pages, 2 figures. Accepted by A&

Risk and expected returns of private equity investments : evidence based on market prices

We estimate the risk and expected returns of private equity investments based on the market prices of exchange traded funds of funds that invest in unlisted private equity funds. Our results indicate that the market expects unlisted private equity funds to earn abnormal returns of about one to two percent. We also find that the market expects listed private equity funds to earn zero to marginally negative abnormal returns net of fees. Both listed and unlisted private equity funds have market betas close to one and positive factor loadings on the Fama-French SMB factor. Private equity fund returns are positively correlated with GDP growth and negatively correlated with the credit spread. Finally, we find that market returns of exchange traded funds of funds and listed private equity funds predict changes in self-reported book values of unlisted private equity funds

Modified dispersion relations and black hole physics

A modified formulation of energy-momentum relation is proposed in the context of doubly special relativity. We investigate its impact on black hole physics. It turns out that such modification will give corrections to both the temperature and the entropy of black holes. In particular this modified dispersion relation also changes the picture of Hawking radiation greatly when the size of black holes approaching the Planck scale. It can prevent black holes from total evaporation, as a result providing a plausible mechanism to treat the remnant of black holes as a candidate for dark matter.Comment: 4 pages, Revtex. Final version to appear in PR

Local Parametric Estimation in High Frequency Data

In this paper, we give a general time-varying parameter model, where the multidimensional parameter possibly includes jumps. The quantity of interest is defined as the integrated value over time of the parameter process $\Theta = T^{-1} \int_0^T \theta_t^* dt$. We provide a local parametric estimator (LPE) of $\Theta$ and conditions under which we can show the central limit theorem. Roughly speaking those conditions correspond to some uniform limit theory in the parametric version of the problem. The framework is restricted to the specific convergence rate $n^{1/2}$. Several examples of LPE are studied: estimation of volatility, powers of volatility, volatility when incorporating trading information and time-varying MA(1).Comment: 67 pages, 4 figure