Additional file 1: of Linking CRISPR-Cas9 interference in cassava to the evolution of editing-resistant geminiviruses

Abstract

Table S1. Virus infection results (confirmation experiment). Table S2. Proportion of ACMV-AC2 H54Q viruses detected by deep-sequencing in N. benthamiana. Table S3. Primer sequences. Table S4. Sequencing Barcodes. Figure S1. Analysis of virus sequences from infected plants at (a) 3 and (b) 8 weeks post infection. Figure S2. Analysis of viral proteins from edited and control populations obtained by single molecule amplicon sequencing at 3 weeks post infection. Figure S3. Analysis of viral proteins from edited and control populations obtained by single molecule amplicon sequencing at 8 weeks post infection. Figure S4. In vitro cleavage assay of the ACMV-AC2 H54Q mutant. Figure S5. Southern blot analysis for number of T-DNA integration events per plant line. * Figure S6. (a) Western blots for Cas9-GFP expression. (b) Raw blot images acquired using an Odyssey CLX imager for anti-Cas9 and anti-Actin probing of protein extracts from Cas9+sgRNA1 lines. (c) Raw blot images for probing Cas9 lines protein extracts with anti-Cas9 and anti-Actin antibodies. Figure S7. Symptom scoring scale. Figure S8. Analysis of full-length virus sequences from infected plants at 8 weeks post infection. (DOCX 6747 kb