Additional file 5: of Exosome-transmitted miR-128-3p increase chemosensitivity of oxaliplatin-resistant colorectal cancer

Abstract

Figure S2. related to Fig. 2. miR-128-3p expression in CRC cell lines and its effect on oxaliplatin resistance. A. Migration and invasion ability of HT29OxR cells transfected with Lv-128 and Ctrl were assessed with a Transwell assay. B. Motility ability of HT29OxR cells transfected with Lv-128 and Ctrl were assessed by wound healing assays. C. Accumulation of Pt in HT29OxR cells transfected with Lv-128 or Ctrl following exposure to 30 μM oxaliplatin treatment for 24 h. D. Total Pt-DNA adduct levels in HT29OxR cells transfected with Lv-128 or Ctrl following exposure to 30 μM oxaliplatin treatment for 24 h. E. The immunofluorescence analysis of nuclear foci for γ-H2AX expression induced by oxaliplatin in HT29OxR cells transfected with Lv-128 or Ctrl after 24 h` oxaliplatin exposure (30 μM). Scale bars, 10 μm. F. RT-qPCR assay was performed to detect the miR-128-3p expression in FHC cells transfected with Lv-128 or Ctrl. (TIF 1535 kb