textjournal article
An ENDOR and HYSCORE Investigation of a Reaction Intermediate in IspG (GcpE) Catalysis
Abstract
IspG is a 4Fe–4S protein that carries out an essential reduction step in isoprenoid biosynthesis. Using electron–nuclear double resonance (ENDOR) and hyperfine sublevel correlation (HYSCORE) spectroscopies on labeled samples, we have specifically assigned the hyperfine interactions in a reaction intermediate. These results help clarify the nature of the reaction intermediate, supporting a direct interaction between the unique fourth Fe in the cluster and C2 and O3 of the ligand- Text
- Journal contribution
- Biophysics
- Biochemistry
- Genetics
- Molecular Biology
- Computational Biology
- Biological Sciences not elsewhere classified
- Chemical Sciences not elsewhere classified
- Physical Sciences not elsewhere classified
- O 3
- CatalysisIspG
- C 2
- isoprenoid biosynthesis
- sample
- cluster
- protein
- IspG
- ligand
- Fe
- nature
- GcpE
- electron
- reduction step
- spectroscopie
- ENDOR
- interaction
- Reaction Intermediate
- sublevel correlation
- HYSCORE Investigation
- resonance
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Last time updated on 16/03/2018
This paper was published in The Francis Crick Institute.
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