Effect of mutations on expression of the 208 core root epidermal genes.

Abstract

<p>(A) Low magnification view of roots from wild-type and homozygous root epidermis mutants. Scale bar: 250 µm. (B) Hierarchical clustering of the 208 core root epidermal genes, based on their relative transcript accumulation in Affymetrix ATH1 microarrays using <i>WER::GFP</i>-expressing cells from three replicates of (left to right) the <i>cpc try</i>, <i>rhd6</i>, <i>gl2</i>, <i>ttg</i>, <i>gl3 egl3</i>, <i>wer myb23</i> mutants and the wild type Columbia. Red = high transcript level; Blue = low transcript level. Asterisks indicate genes significantly affected in the <i>rhd6</i> mutant background. (C) Hierarchical clustering of the 208 core root epidermal genes, based on their relative transcript accumulation (averaged from three replicates) in Affymetrix ATH1 microarrays using <i>WER::GFP</i>-expressing cells from (left to right) <i>cpc try</i>, <i>rhd6</i>, <i>csld3</i>, <i>mrh3</i>, <i>gl2</i>, <i>gl3 egl3</i>, <i>ttg</i>, <i>lrx1</i>, <i>cobl9</i>, <i>rhd2</i>, <i>ire</i>, <i>mrh1</i>, <i>mrh2</i>, <i>wild type</i>, <i>cow1</i>, <i>myc1</i>, <i>bhlh66</i>, and <i>wer myb23</i>. Red = high transcript level; Blue = low transcript level. Heirarchical clustering of the complete set of three replicates for each line is presented as <a href="http://www.plosgenetics.org/article/info:doi/10.1371/journal.pgen.1002446#pgen.1002446.s005" target="_blank">Figure S5</a>.</p