Effect of siRNA-mediated <i>SPATS2L</i> knockdown on β₂-adrenergic receptor levels in HASM cells.

Abstract

<p>(A) Knockdown efficiency of two <i>SPATS2L</i> siRNAs. Quantitative real-time PCR was done on RNAs extracted from HASM cells transfected with control non-targeting (NT) or <i>SPATS2L</i>-specific siRNAs. (B) Western blot analysis of β₂AR protein in <i>SPATS2L</i> knockdown HASM cells. Three independent experiments (siRNA transfection and Western blot analysis) were done in HASM cells. The upper panel is a representative of the three Western blots. Quantification of the β₂AR protein amount (normalized against the control β-actin protein) from three Western blots is shown in the lower panel.</p