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Effect of siRNA-mediated <i>SPATS2L</i> knockdown on β<sub>2</sub>-adrenergic receptor levels in HASM cells.

By Blanca E. Himes (153105), Xiaofeng Jiang (153107), Ruoxi Hu (153109), Ann C. Wu (153111), Jessica A. Lasky-Su (153113), Barbara J. Klanderman (153115), John Ziniti (153117), Jody Senter-Sylvia (153119), John J. Lima (153121), Charles G. Irvin (153123), Stephen P. Peters (153125), Deborah A. Meyers (153130), Eugene R. Bleecker (153133), Michiaki Kubo (128298), Mayumi Tamari (132418), Yusuke Nakamura (25445), Stanley J. Szefler (153142), Robert F. Lemanske Jr. (153147), Robert S. Zeiger (153151), Robert C. Strunk (153154), Fernando D. Martinez (153157), John P. Hanrahan (153160), Gerard H. Koppelman (153163), Dirkje S. Postma (101740), Maartje A. E. Nieuwenhuis (153166), Judith M. Vonk (71591), Reynold A. Panettieri Jr. (153171), Amy Markezich (153173), Elliot Israel (153177), Vincent J. Carey (153183), Kelan G. Tantisira (153187), Augusto A. Litonjua (27333), Quan Lu (153192) and Scott T. Weiss (153197)

Abstract

<p>(A) Knockdown efficiency of two <i>SPATS2L</i> siRNAs. Quantitative real-time PCR was done on RNAs extracted from HASM cells transfected with control non-targeting (NT) or <i>SPATS2L</i>-specific siRNAs. (B) Western blot analysis of β<sub>2</sub>AR protein in <i>SPATS2L</i> knockdown HASM cells. Three independent experiments (siRNA transfection and Western blot analysis) were done in HASM cells. The upper panel is a representative of the three Western blots. Quantification of the β<sub>2</sub>AR protein amount (normalized against the control β-actin protein) from three Western blots is shown in the lower panel.</p

Topics: Medicine, Genetics, sirna-mediated, knockdown, receptor, levels, hasm
Year: 2012
DOI identifier: 10.1371/journal.pgen.1002824.g004
OAI identifier: oai:figshare.com:article/284040
Provided by: FigShare
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