Identification
and Quantification
of 1‑Hydroxybutene-2-yl Mercapturic Acid in Human Urine by
UPLC- HILIC-MS/MS as a Novel Biomarker for 1,3-Butadiene Exposure
- Publication date
- 2012
- Publisher
Abstract
1,3-Butadiene (BD) is a Class 1 carcinogen present at
workplaces,
in polluted air, in automobile exhaust, and in tobacco smoke. 2-Hydroxybutene-1-yl
mercapturic acid (2-MHBMA) is a urinary metabolite often measured
as a biomarker for exposure to BD. Here, we show for the first time
that an additional MHBMA isomer is present at significant amounts
in human urine, 1-hydroxybutene-2-yl mercapturic acid (1-MHBMA). For
its quantification, a highly sensitive UPLC-HILIC-MS/MS method was
developed and validated. Analyzing urinary samples of 183 volunteers,
we demonstrate that 1-MHBMA is a novel and potentially more reliable
biomarker for BD exposure than the commonly analyzed 2-MHBMA