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Identification and Quantification of 1‑Hydroxybutene-2-yl Mercapturic Acid in Human Urine by UPLC- HILIC-MS/MS as a Novel Biomarker for 1,3-Butadiene Exposure

By Katharina Sterz (2053330), Gerhard Scherer (2053333), Jan Krumsiek (127460), Fabian J. Theis (2053339) and Josef Ecker (2053336)


1,3-Butadiene (BD) is a Class 1 carcinogen present at workplaces, in polluted air, in automobile exhaust, and in tobacco smoke. 2-Hydroxybutene-1-yl mercapturic acid (2-MHBMA) is a urinary metabolite often measured as a biomarker for exposure to BD. Here, we show for the first time that an additional MHBMA isomer is present at significant amounts in human urine, 1-hydroxybutene-2-yl mercapturic acid (1-MHBMA). For its quantification, a highly sensitive UPLC-HILIC-MS/MS method was developed and validated. Analyzing urinary samples of 183 volunteers, we demonstrate that 1-MHBMA is a novel and potentially more reliable biomarker for BD exposure than the commonly analyzed 2-MHBMA

Topics: Biochemistry, Medicine, Microbiology, Cell Biology, Neuroscience, Pharmacology, Biotechnology, Sociology, Immunology, Hematology, Biological Sciences not elsewhere classified, Chemical Sciences not elsewhere classified, biomarker, Human Urine, acid, 183 volunteers, BD exposure, automobile exhaust, UPLC, MHBMA isomer, tobacco smoke, Novel Biomarker, Hydroxybutene, mercapturic, Class 1 carcinogen
Year: 2012
DOI identifier: 10.1021/tx3002862.s001
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Provided by: FigShare
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