Snm1B interacts with PSF2.

Abstract

<p><i>A,</i> Diagram of Snm1B mutants generated. The two putative PSF2-binding regions (PSF2 BR1 and BR2) are denoted by grey bars. WT, wild type. * denotes a mutation that reduces TRF2 binding. <i>B,</i> Growth of yeast expressing PSF2-Gal4AD (PSF2) and the Gal4BD (vector), PSF2-GalAD and Gal4BD fused to the C-terminus of Snm1B (Snm1B), or positive control TRF2-Gal4AD (TRF2) and Snm1B-GalBD on SD/-leu/-trp or SD/-ade/-his/-leu/-trp dropout media in the presence of x-α-gal. <i>C, E,</i> The indicated Flag epitope-tagged Snm1B (Flag-Snm1B) or <i>D,</i> the indicated GFP-tagged Flag-Snm1B (GFP-Flag-Snm1B) transiently expressed in 293T cells in the presence of ectopic Myc epitope-tagged PSF2 (Myc-PSF2) were immunoprecipitated (IP) with either an anti-Flag or anti-GFP antibody and immunoblotted (IB) with an anti-Flag antibody to visualize Flag-Snm1B or an anti-Myc antibody to visualize Myc-PSF2. Data are representative of at least two independent experiments.</p