Synthesis of 5‑Hydroxymethyl‑, 5‑Formyl‑, and 5‑Carboxycytidine-triphosphates and Their Incorporation into Oligonucleotides by Polymerase Chain Reaction
Abstract
The synthesis of the triphosphates of 5-hydroxymethyl-, 5-formyl-, and 5-carboxycytidine and the incorporation of these building blocks into long DNA fragments using the polymerase chain reaction (PCR) are reported. In this way DNA fragments containing multiple hmC, fC, and caC nucleobases are readily accessible- Text
- Journal contribution
- Biophysics
- Biochemistry
- Medicine
- Microbiology
- Cell Biology
- Genetics
- Molecular Biology
- Biotechnology
- Immunology
- Mental Health
- Infectious Diseases
- Chemical Sciences not elsewhere classified
- Physical Sciences not elsewhere classified
- building blocks
- PCR
- Hydroxymethyl
- way DNA fragments
- caC nucleobases
- formyl
- hydroxymethyl
- triphosphate
- fC
- Incorporation
- Oligonucleotide
- Polymerase Chain ReactionThe synthesis
- DNA fragments
- incorporation
- polymerase chain reaction
- carboxycytidine
- Formyl
- Synthesi
- hmC
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This paper was published in FigShare.
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