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ChemLTP increases SUMO-1 and Ubc9 mRNA.

By Nadia Jaafari (289396), Filip A. Konopacki (289398), Thomas F. Owen (289399), Sriharsha Kantamneni (289400), Philip Rubin (289401), Tim J. Craig (289403), Kevin A. Wilkinson (289405) and Jeremy M. Henley (289407)


<p>A) Fluorescent in situ hybridization using cRNA probes for SUMO-1, SUMO-2 and Ubc9 (red) in hippocampal neurons co-stained with the dendritic marker MAP-2 (green). ChemLTP increased levels of SUMO-1 and Ubc9 but not SUMO-2 mRNA in soma and dendrites. Mean fluoresence intensity was quantified in at least 3 independent experiments with 10 neurons per condition and 3 ROIs per neuron using ImageJ. B) ChemLTP increases levels of the mRNA binding protein CPEB1 (red) in hippocampal cultured neurons co-immunostained with MAP2 (green). Three independent experiments with 8 neurons per condition and 4 ROIs per neuron were quantified using JACoP plugin in ImageJ. Statistical significance was determined using ANOVA *p<0.02; **p<0.005. Scale bars – 20 µm.</p

Topics: Biochemistry, Neuroscience, Physiology, increases, sumo-1, ubc9
Year: 2013
DOI identifier: 10.1371/journal.pone.0052345.g003
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Provided by: FigShare
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