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Inhibitory effect of genistein on TNF-α and IL-6 mRNA levels in LPS-treated RAW 264.7 macrophages.

By Guiyuan Ji (291243), Yupei Zhang (291244), Qinhe Yang (291245), Shaobin Cheng (291246), Jing Hao (291247), Xihong Zhao (291248) and Zhuoqin Jiang (291249)

Abstract

<p>Cells were pretreated with genistein (0.1 to 10 µM) for 1 h and then incubated with LPS (1 µg/mL) for 24 h. Cells were collected, and IL-6 and TNF-α mRNA levels were determined by qRT-PCR and normalized to β-actin. Each column represents the mean ± S.E.M of triplicate experiments. **<i>P</i><0.01 vs. control, <sup>##</sup><i>P</i><0.01,<sup> #</sup><i>P</i><0.05 vs. LPS alone.</p

Topics: Biochemistry, Genetics, Physiology, Pharmacology, Chemistry, Immunology, genistein, il-6, mrna, levels, lps-treated
Year: 2013
DOI identifier: 10.1371/journal.pone.0053101.g002
OAI identifier: oai:figshare.com:article/185725
Provided by: FigShare
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