Simulating <i>in vivo</i> study of the effect of reduced dynorphin on burst and silence duration.

Abstract

<p>Our data simulates in vivo analysis using data from multiple cells by generating 100 model cells with random variation in the seven non-synaptic parameters used to fit the varied cells in <a href="http://www.ploscompbiol.org/article/info:doi/10.1371/journal.pcbi.1002740#pcbi-1002740-g003" target="_blank">Figure 3</a> (<a href="http://www.ploscompbiol.org/article/info:doi/10.1371/journal.pcbi.1002740#pcbi-1002740-t004" target="_blank">Table 4</a>). The dynorphin antagonist was simulated by reducing k<i>_D</i> by 15% in each generated cell. This is sufficient to have a large effect on spike rate (A) and the burst duration histogram and hazard (B), comparable to the <i>in vivo</i> results in Brown et al 2006 <a href="http://www.ploscompbiol.org/article/info:doi/10.1371/journal.pcbi.1002740#pcbi.1002740-Brown4" target="_blank">[28]</a> (c.f. their <a href="http://www.ploscompbiol.org/article/info:doi/10.1371/journal.pcbi.1002740#pcbi-1002740-g005" target="_blank">figure 5</a>) but also shows very little effect on the silence (inter-burst interval) duration (C), similar to what they observe, suggesting that their results do not, as they interpret, exclude a role for dynorphin in generating inter-burst silence.</p