Effect of extracellularacyl-CARs on I_hERG.

Abstract

<p>I_hERG-V relationships,in all graphs, the filled squares represent the current in the absence of acyl-CAR in the pipette (control, n = 12 cells). <b>Aa</b>, effect of 3 µM C8-CAR (empty circles, n = 8 cells) or 3 µM C10-acyl-CAR (filled circles, n = 7 cells); <b>Ab</b>, typical example of C8-CAR (red) compared with PSS (black). <b>Ba,</b> effect of 3 µM C18-CAR (filled circles, n = 9 cells) on I_hERG-V relationship. The inset shows the effect of 3 µM C18-CAR on end-pulse hERG current elicited by a depolarisation to −10 mV from a holding voltage of −70 mV, obtained on a representative cell (the arrows indicate the current obtained at −10 mV during an IV protocol, see <a href="http://www.plosone.org/article/info:doi/10.1371/journal.pone.0041686#s2" target="_blank">Methods</a>), ***, p<0.001. **, p<0.01.<b>Bb</b>, typical example of C18-CAR (red) compared with PSS (black).</p