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Schematic of Adherent Cell-SELEX (AC-SELEX) Procedure.

By Jessica C. Graham (325676) and Helmut Zarbl (216223)

Abstract

<p>A starting library of >10<sup>15</sup> aptamers was utilized and eighteen rounds of AC-SELEX were carried out to purify and amplify the aptamers which recognized epitopes present on HF cells and not present on HeLa cells. The starting library was first incubated with the HeLa cell line and the unbound aptamers were recovered and utilized for the AC-SELEX procedure.</p

Topics: Cell Biology, Cancer, Infectious Diseases, adherent, cell-selex
Year: 2013
DOI identifier: 10.1371/journal.pone.0036103.g001
OAI identifier: oai:figshare.com:article/320458
Provided by: FigShare
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