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Lentivirus-mediated miR-223 over-expression in HeLa cells.

By Cheng You Jia (340599), Hui Hui Li (340602), Xu Chao Zhu (340603), Yi Wei Dong (340604), Da Fu (114238), Qian Lei Zhao (340606), Wei Wu (27708) and Xing Zhong Wu (340608)

Abstract

<p>(A) Lentivirus vector pLL3.7-miR-223 was packaged in HEK-293T cells which was less 20 generations. The packaging efficiency was evaluated by GFP fluorescent signal. Original magnification 10 × (B) HeLa cells were infected with the Lentivirus which was packaged and prepared from HEK-293T cells. The GFP fluorescent signal could be stably observed 72 hours after infection. EV group stood for vector control at the left and pLL3.7-miR-223 infection group was at the right. Original magnification 20 × (C) The infected HeLa cells were sorted by FACS. The fluorescence-positive cells were collected in either EV control (left) or miR-223 (right) group. Original magnification 10× (D,E) Mir-223 was over-expressed in HeLa cells and confirmed by both stem-loop RT-PCR (D) and quantitative PCR (E). NV: no virus control, EV: empty virus vector control, Input: positive mature miR-223.</p

Topics: Biophysics, Biochemistry, Physics, Cell Biology, Molecular Biology, Cancer, mir-223, over-expression, hela
Year: 2013
DOI identifier: 10.1371/journal.pone.0027008.g001
OAI identifier: oai:figshare.com:article/387868
Provided by: FigShare
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