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Epitope-specific CD4<sup>+</sup> and CD8<sup>+</sup> T-cell activation in peptide-immunized HLA-A*0201 transgenic mice.

By Hsiao-Yun Shao (289558), Yi-Wen Lin (143406), Shu-Ling Yu (343034), Hsiang-Yin Lin (335160), Ebenezer Chitra (335162), Yung-Chen Chang (343035), Charles Sia (335164), Pele Chong (296102), Ming-Tao Hsu (343036), Olivia L. Wei (343037) and Yen-Hung Chow (325993)


<p>Splenocytes were isolated on day 17 from mice immunized twice subcutaneously with the peptides 3, 13, 14, 17, 23, or vehicle at day 0 and day 10. The splenocytes were labeled with CFSE and cultured in the presence or absence of 10 µg/mL of the respective peptides for 8 days. Proliferation of CD4<sup>+</sup> (A) or CD8<sup>+</sup> (B) lymphocytes in response to the different CD8 epitopes was analyzed by flow cytometry using anti-CD4 or CD8 antibodies conjugated with PE-Cy5. Results are presented as cell division index (CDI) as described in the <a href="" target="_blank">Materials and Methods</a>. (C) The splenocytes were stimulated <i>in vitro</i> with or without the peptides and were stained with anti-CD8 antibody conjugated with FITC, and then fixed and stained for intracellular IFN-γ using PE-conjugated anti-IFN-γ antibody. The percentage of CD8<sup>+</sup> IFN-γ<sup>+</sup> T cells was calculated. *(<i>p</i><0.05) and **(<i>p</i><0.01) indicate they are significantly different from the unstimulated splenocytes. Data is representative of results derived from three independent experiments.</p

Topics: Immunology, Virology, t-cell, activation, peptide-immunized, transgenic
Year: 2013
DOI identifier: 10.1371/journal.pone.0025500.g002
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Provided by: FigShare
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