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IL-10 DC generated in the presence of IFN-α induced an enhanced T cell activation.

By Nicole Bacher (347165), Edith Graulich (347166), Helmut Jonuleit (259744), Stephan Grabbe (103633) and Kerstin Steinbrink (347167)


<p>(A, B) CD4<sup>+</sup> or CD8<sup>+</sup> T cells were stimulated in primary culture with allogeneic mDC, IL-10 DC or IL-10 DC cultured with 10<sup>4</sup> U/mL IFN-α. (A) T cell proliferation after restimulation was assessed as described and pooled data of 6 (CD4<sup>+</sup> T cells) or 3 (CD8<sup>+</sup> T cells) independent experiments are demonstrated. Proliferation is demonstrated in % +/−SD (normalized to 100% proliferation induced by control mDC). (B) IFN-γ and IL-10 production were detected in the supernatants of CD4<sup>+</sup> T cells at day 5 of primary culture by ELISA. Mean values of cytokine levels in pg/ml +/− SD of 6 (IFN-γ) or 5 (IL-10) independent experiments are shown. * p<0.05; ** p<0.01; n.s. not significant.</p

Topics: Immunology, dc, generated, induced, enhanced
Year: 2013
DOI identifier: 10.1371/journal.pone.0022763.g002
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Provided by: FigShare
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