<i>In vitro</i> osteogenesis accessed in three-month-old mice.

Abstract

<p>Bone marrow cells were obtained from WT female or male mice and cultured in osteogenic conditions for 14 days or 21 days and then stained for alkaline phosphatase (ALP, A and B, top pannels) or alizarin red (AR, A and B, lower pannels). The ALP level was quantified by absorbance at OD 410 nm (C)and normalized by total cell protein. The AR level was quantified by absorbance at OD 490 nm (D).</p