Role of proliferation and apoptosis in Araucana<i>^Rp</i> tailbud development.

Abstract

<p>EdU labeled proliferating cells in tailbud sagittal sections at HH15-20 in controls. (A,C,E,G) and Araucana<i>^Rp</i> (B,D,F,H) embryo tailbud. Green is EdU labeled proliferating cells, red is TO-PRO-3 Iodide labeled nuclei. Arrows denote decreased regions of proliferation in Araucana<i>^Rp</i> compared to controls. (I) Quantification of all TBM cells for control and Araucana<i>^Rp</i> labeled with TO-PRO-3 Iodide. (J) Quantification of proliferating TBM cells for control and Araucana<i>^Rp</i> labeled with EdU. TUNEL labeled apoptotic cells in tailbud sagittal sections from HH stages 15–20 of controls. (K,M,O,Q) and Araucana<i>^Rp</i> (L,N,P,R) embryo tailbud. Red is TUNEL labeled apoptotic cells, blue is Hoechst labeled nuclei. Arrows denote increased areas of apoptosis in Araucana<i>^Rp</i> compared to controls. (S) Quantification of apoptotic TUNEL labeled cells in control and Araucana<i>^Rp</i> TBM. Araucana<i>^Rp</i> have significantly more TUNEL positive cells beginning at HH17 than controls. T-test, asterisk marks p<0.05. Control-Black, Araucana<i>^Rp</i>-Grey. Same embryo (two different sections) was used for both TUNEL and EdU labeling. Anterior is towards the top, dorsal is towards the left in all sections.</p