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NO stabilizes and upregulates ULK1 protein expression.

By Junhui Xing (679259), Hongtao Liu (114450), Huabing Yang (679260), Rui Chen (95310), Yuguo Chen (580875) and Jian Xu (31545)

Abstract

<p>(A) HUVECs were transfected with GFP or eNOS adenovirus for 48 h; (B) HUVECs were treated with A23187 (1 µM) for the indicated time; (C) HUVECs were treated with NONOate (50 µM) for the indicated time; (D) HUVECs were treated with CHX (5 µM) for the indicated time, followed by incubation of NONOate (50 µM) for 4 h; (E) HUVECs were treated with CHX (5 µM) for the indicated time, followed by incubation of A23187 (1 µM) for 4 h. The western blots are representative of three independent experiments. *represents <i>p</i><0.05 vs control (<i>n</i> = 3); NS, not significant. GFP, green fluorescent protein; eNOS, endothelial nitric oxide synthase; Ad, Adenovirus; CHX, cycloheximide.</p

Topics: Biological Sciences, mef, chx, SIRT 1 protein expression, SIRT 1 turnover, 26 S proteasome functionality, Nitric Oxide Synthase, mg, ULK 1, ULK 1 siRNA treatment, E 3 ligase, SIRT 1 protein levels, Autophagy SIRT 1, SIRT 1 protein breakdown, SIRT 1 protein, ogt, SIRT 1 reduction, proteasomal subunit Rpt 2.
Year: 2014
DOI identifier: 10.1371/journal.pone.0116165.g002
OAI identifier: oai:figshare.com:article/1279864
Provided by: FigShare
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