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<i>OLE-lacZ</i> and <i>OLEa-lacZ</i> transgenes are predominantly expressed in oligodendrocytes.

By Matthias Weider (2217631), Amélie Wegener (2217634), Christian Schmitt (543653), Melanie Küspert (2217628), Simone Hillgärtner (2217625), Michael R. Bösl (189854), Irm Hermans-Borgmeyer (478560), Brahim Nait-Oumesmar (2217637) and Michael Wegner (12071)

Abstract

<p><b>(A)</b> The transgenic constructs consisting of OLE or OLEa, the minimal Hsp68 promoter (hsp68), the lacZ marker gene (lacZ) and a SV40 polyA signal (pA) are schematically shown. (<b>B</b>) Five founders were obtained after pronucleus injection of <i>OLE-lacZ</i> and <i>OLEa-lacZ</i> that inherited the transgene to their progeny and were expanded to lines. Three of them (OLE#4, OLE#5 and OLEa#5) did not show transgene expression. All other lines exhibited strong and selective expression in oligodendroglial cells with more than 80% of β-galactosidase positive cells in the CNS corresponding to cells of this lineage (+ and ++). In comparison, less than 20% of β-galactosidase positive cells in the CNS were identified as astrocytes (±) in several lines. Only OLE#2 and OLEa#4 exhibited occasional β-galactosidase labelling of neurons (±). A higher fraction of the oligodendroglial population expressed the transgene in lines OLE#1–3 (++) than in OLEa#1–4 (+). Outside the CNS, weak additional transgene expression was observed for several lines within the DRG, in cartilage or in vasculature as indicated.</p

Topics: Cell Biology, Genetics, Molecular Biology, Neuroscience, Immunology, Developmental Biology, Biological Sciences not elsewhere classified, Single Transcription Factor, dorsal root ganglia, Olig 2 gene, myelin gene expression, transcription factor Sox 10, cell conversion strategies, oligodendrocyte precursor cell stage, Convert Satellite Glia, Sox 10
Year: 2015
DOI identifier: 10.1371/journal.pgen.1005008.g009
OAI identifier: oai:figshare.com:article/2676817
Provided by: FigShare
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