The effects of silencing Pik3ip1 on the PI3K/AKT/mTOR pathway.

Abstract

<p>NRCMs were transfected with the indicated siRNA for 24 h and subsequently serum-starved for 24 h. <b>(A)</b> PI3K activity (p110α) in the siNegative and siPik3ip1-transfected NRCMs. Western blot analyses of samples from siNegative and siPik3ip1-transfected NRCMs were performed after 48 h. (<b>B–E</b>) Representative immunoblot images of phosphorylated protein and total protein (upper), and the ratio of phosphorylated protein/total protein (bottom) for (<b>B</b>) AKT, (<b>C</b>) mTOR, (<b>D</b>) p70s6k, and (<b>E</b>) eEF2. <b>(F)</b> ERK 1/2 (n = 3, * p < 0.05 and ** p < 0.01, t test)</p