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The effects of silencing Pik3ip1 on the PI3K/AKT/mTOR pathway.

By Hong Ki Song (171611), Jiyeon Kim (202174), Jong Sub Lee (715348), Kyoung Jin Nho (715349), Hae Chang Jeong (564627), Jihwa Kim (715350), Youngkeun Ahn (564628), Woo Jin Park (142218) and Do Han Kim (23257)

Abstract

<p>NRCMs were transfected with the indicated siRNA for 24 h and subsequently serum-starved for 24 h. <b>(A)</b> PI3K activity (p110α) in the siNegative and siPik3ip1-transfected NRCMs. Western blot analyses of samples from siNegative and siPik3ip1-transfected NRCMs were performed after 48 h. (<b>B–E</b>) Representative immunoblot images of phosphorylated protein and total protein (upper), and the ratio of phosphorylated protein/total protein (bottom) for (<b>B</b>) AKT, (<b>C</b>) mTOR, (<b>D</b>) p70s6k, and (<b>E</b>) eEF2. <b>(F)</b> ERK 1/2 (n = 3, * p < 0.05 and ** p < 0.01, t test)</p

Topics: Uncategorised, pah, Inhibiting PI 3K Pathway Cardiac hypertrophy, Pik 3ip shares homology, Pik 3ip deficiency, p 110a subunit, phh, PI 3K activity, Pik 3ip Modulates Cardiac Hypertrophy, Pik 3ip attenuated PI 3K, PI 3K kinase B, nrcm, Pik 3ip, akt, PI 3K
Year: 2015
DOI identifier: 10.1371/journal.pone.0122251.g003
OAI identifier: oai:figshare.com:article/1362884
Provided by: FigShare
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